ISfinder Mirror Site

ISSysp8

Family ISKra4
Group ISMich2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_019680	ND	Synechococcus sp.	Synechococcus sp. PCC 6312

DNA section

IS Length : 1187 bp

Ends

IR Length : 30/37

IRL : GGGTGCATCTCACTTAGGAGGCGAGTATATATACTCAGTGCGAGAGTGAG
IRR : GGGAGCATCTCAATTAGGTAATAAGTATATATACTCAAGGAGCAAAAGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TAATGTTTCT	GATTTAAC	AGTCTTAAGT	8

DNA sequence

GGGTGCATCTCACTTAGGAGGCGAGTATATATACTCAGTGCGAGAGTGAGTTAAAGCATTGAGAATAAACGTAATGTAAATCAAGCCAGCCCAATGGATA
GACAGACGCAATCAGAACTCCAGACTCATTTAAATGCCATTGCGGCCATCCTGTTTAAAGAAGCCGACCCAGCAGACCTGAAAACCTTAGGCGGGATAGA
AAAGAGTGTGCGAGGCTTAGCCCAGGAACATGTTTTGCCCCAATTAGGAATTTTTTATCGACAGCGCAACAGAGACGAGACCTGGAAAAACCCGAGTCAT
AACGAGTATTCTGGGGGAGCTGCACCTAAGTCAGTCTCAAGCAGAGCAATTGCATGTCAAGCCAAGGAGTCGTTGGAGTCCATACCTAGAGCGTTGTTGT
CTCTTAGTGAGTGCCAATGCCTCCTATCAGCGAGCCGAAGAAGATATCGAGGTGTTGACGGGGATAACGGTGTCCCACAGCACCCTCCAGCGTCTGGTGC
AGCGTCAGGCGTGGCCGGAGGTGGTATTGACCCGCCCCATTGAAGAGATGAGCTTAGATGGGGGGATGATTCGCCTGCGCACCGAAGCAGGAACCCCCTG
TGAGTGGCGAGACTACAAAGCCTTAGCGATTCATGACCAGGCCGGGGTTGCCTTCTTTAAGGACAATGAGGGCTTAATCCATTGGGCCAATCGTCAACCT
CGAACTACTCCCTTTACCGGTTTGGGGGATGGGCATAACGGGGTCTGGAACATTCTGACTCAGATAGGGACGACCCGAGAGCGGATTGAAATCTTGGATT
GGTTTCATTTGATGGAAAACGCCCATAAGGTGCAGGCGACACCCACTCAATTAAAAGCCGTGCGAGACCATCTCTGGCAGGGACAGGTGACGCTGGCCAT
TCAAGTGTTACAGAACTATCGGTGTCTGGGAGCCTCGACCTTTATCCAGTATTTAAGGCATCATCAACACCGAATCATTCACTACCAGGCCTGGCAGGCG
GCGGGGCACTCGATTGGCTCTGGACAGGTTGAGTCCTTCGTGAAGCAGATTAGTCTCCGGGTGAAGTTGCCAGGGGCGCAATGGTTGCCGCAGAATGTTC
CTAAGCTATTGAAACATCGCTGTGCTTATCTCAATGGCGCTTTTGCTCCTTGAGTATATATACTTATTACCTAATTGAGATGCTCCC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
450 bp	149 aa	94	543	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDRQTQSELQTHLNAIAAILFKEADPADLKTLGGIEKSVRGLAQEHVLPQLGIFYRQRNRDETWKNPSHNEYSGGAAPKSVSSRAIACQAKESLESIPRA
LLSLSECQCLLSASRRRYRGVDGDNGVPQHPPASGAASGVAGGGIDPPH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
906 bp	301 aa	248	1153	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

EFFIDSATETRPGKTRVITSILGELHLSQSQAEQLHVKPRSRWSPYLERCCLLVSANASYQRAEEDIEVLTGITVSHSTLQRLVQRQAWPEVVLTRPIEE
MSLDGGMIRLRTEAGTPCEWRDYKALAIHDQAGVAFFKDNEGLIHWANRQPRTTPFTGLGDGHNGVWNILTQIGTTRERIEILDWFHLMENAHKVQATPT
QLKAVRDHLWQGQVTLAIQVLQNYRCLGASTFIQYLRHHQHRIIHYQAWQAAGHSIGSGQVESFVKQISLRVKLPGAQWLPQNVPKLLKHRCAYLNGAFA
P

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1060 bp	353 aa	94	1153	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDRQTQSELQTHLNAIAAILFKEADPADLKTLGGIEKSVRGLAQEHVLPQLGIFFIDSATETRPGKTRVITSILGELHLSQSQAEQLHVKPRSRWSPYLE
RCCLLVSANASYQRAEEDIEVLTGITVSHSTLQRLVQRQAWPEVVLTRPIEEMSLDGGMIRLRTEAGTPCEWRDYKALAIHDQAGVAFFKDNEGLIHWAN
RQPRTTPFTGLGDGHNGVWNILTQIGTTRERIEILDWFHLMENAHKVQATPTQLKAVRDHLWQGQVTLAIQVLQNYRCLGASTFIQYLRHHQHRIIHYQA
WQAAGHSIGSGQVESFVKQISLRVKLPGAQWLPQNVPKLLKHRCAYLNGAFAP

Blast result :

Comments

ISSysp8 is 68% aa similar to ISCysp25.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2013)
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C., Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and Kerfeld,C. (2012) Direct submission GenBank.