ISAcas2
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AFEJ01000480 | ND | Acaryochloris sp. | Acaryochloris sp. CCMEE 5410 |
DNA section
IS Length : 1164 bp
Ends
IR Length : 25/30
IRL : GGGAGCGTGTCACTTCTTTGAGAGGAAAATGATAATTGATCTTAGATGAT
IRR : GGGAGCGTGTCACTTTTTCAGGAGAAAAATAGAGTCAAGTTGTCGATTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACTCTTTCT | ATCTAGCTTT | 0 |
DNA sequence
GGGAGCGTGTCACTTCTTTGAGAGGAAAATGATAATTGATCTTAGATGATCCCTCTTATTGACTATGACACCAGAAGAAGAACAAGAATTCAATGCTTGC
GTCACACGCATTTCAGAACTGCTGTATCAAGACTCCCAATCCCAGTCTCTGCCCATGAAGACTTTGGCAGAGATTGAATGCACCGTTCGGACTCAACTGC
AAACTCATGTGTCGCCTCAAATGGGTCTTTTTTTATCGACCAAGTTAGCCCGCCAGATGTCGGAGAATATCGACGGACCTTAAAAAGCATTCTGGGCAAA
CTCAACCTCACTCGGACCCAAGCAACAGCCCTCAAGGTCAAGCCCAACAGTCAGTTAAGCCCCTACTTAGAGATGTGCGCCCTGCGTATTAGTGCCAATG
TTTCCTATGCCCATGCATCAGAGGATCTAGCAGTTTTAACGGGTATCACAGTCAGCTCCAAAACGCAGCAGCGTCTCGTTCATCGTCAAACCTTCAGTCC
ACCCTCTTTGACGCAAGGAGTCACTCAGCTCAGCTTAGATGGGGGCAATATTCGGTTGATTACTCCAAAAGGGGAACCCTGCCACTGGCGTGGTTATAAA
GCAGTTCGCATCAATGGCGATAGGGTTGGCTTGGCCTACTATCAAGACCATACTTCCCTCTGCCTGGCGGTGAACCGCTTCAGATTTGCTGCAAGGGTTT
ACTGTCTTGGGGATGGTCATGTTGGGATTTGGAAGCTTTACCGACAGATGAGACTGCCAACTCAACAGGAGCAGATCCTAGACTGGTTCCACTTGATGGA
GAATTTACATAAAGTCGGTGGGTCACTCAAACGGCTGCAGCAAGCTGAAACCTTTTTGTGGAGAGGGAAGATTGATGAGGCCATCGATTTGTTCTGTGGT
TTGAGCAAACCTCAGGCTAAACGGTTCTGTCAGTATTTGCGAGACCACCGAGAGCGGATTCCCAACTATGGTTACTATCAAGCTGAGGGAATACCCATTG
GCTCTGGTGCCGTTGAGTCGCTTGTTAAGCAAATTGACCGAAGGACAAAGATTTCAGGTGCGCAATGGCAAGAAAAACATATCCCCAAAGTCCTAGCTCA
TCGCTGTGCCTATCTCAATCGACAACTTGACTCTATTTTTCTCCTGAAAAAGTGACACGCTCCC
GTCACACGCATTTCAGAACTGCTGTATCAAGACTCCCAATCCCAGTCTCTGCCCATGAAGACTTTGGCAGAGATTGAATGCACCGTTCGGACTCAACTGC
AAACTCATGTGTCGCCTCAAATGGGTCTTTTTTTATCGACCAAGTTAGCCCGCCAGATGTCGGAGAATATCGACGGACCTTAAAAAGCATTCTGGGCAAA
CTCAACCTCACTCGGACCCAAGCAACAGCCCTCAAGGTCAAGCCCAACAGTCAGTTAAGCCCCTACTTAGAGATGTGCGCCCTGCGTATTAGTGCCAATG
TTTCCTATGCCCATGCATCAGAGGATCTAGCAGTTTTAACGGGTATCACAGTCAGCTCCAAAACGCAGCAGCGTCTCGTTCATCGTCAAACCTTCAGTCC
ACCCTCTTTGACGCAAGGAGTCACTCAGCTCAGCTTAGATGGGGGCAATATTCGGTTGATTACTCCAAAAGGGGAACCCTGCCACTGGCGTGGTTATAAA
GCAGTTCGCATCAATGGCGATAGGGTTGGCTTGGCCTACTATCAAGACCATACTTCCCTCTGCCTGGCGGTGAACCGCTTCAGATTTGCTGCAAGGGTTT
ACTGTCTTGGGGATGGTCATGTTGGGATTTGGAAGCTTTACCGACAGATGAGACTGCCAACTCAACAGGAGCAGATCCTAGACTGGTTCCACTTGATGGA
GAATTTACATAAAGTCGGTGGGTCACTCAAACGGCTGCAGCAAGCTGAAACCTTTTTGTGGAGAGGGAAGATTGATGAGGCCATCGATTTGTTCTGTGGT
TTGAGCAAACCTCAGGCTAAACGGTTCTGTCAGTATTTGCGAGACCACCGAGAGCGGATTCCCAACTATGGTTACTATCAAGCTGAGGGAATACCCATTG
GCTCTGGTGCCGTTGAGTCGCTTGTTAAGCAAATTGACCGAAGGACAAAGATTTCAGGTGCGCAATGGCAAGAAAAACATATCCCCAAAGTCCTAGCTCA
TCGCTGTGCCTATCTCAATCGACAACTTGACTCTATTTTTCTCCTGAAAAAGTGACACGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 219 bp | 72 aa | 65 | 283 | + | No |
Description : First part of the transposase
ORF sequence :
MTPEEEQEFNACVTRISELLYQDSQSQSLPMKTLAEIECTVRTQLQTHVSPQMGLFLSTKLARQMSENIDGP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 978 bp | 325 aa | 178 | 1155 | + | No |
Description : Second part of the transposase
ORF sequence :
MHRSDSTANSCVASNGSFFIDQVSPPDVGEYRRTLKSILGKLNLTRTQATALKVKPNSQLSPYLEMCALRISANVSYAHASEDLAVLTGITVSSKTQQRL
VHRQTFSPPSLTQGVTQLSLDGGNIRLITPKGEPCHWRGYKAVRINGDRVGLAYYQDHTSLCLAVNRFRFAARVYCLGDGHVGIWKLYRQMRLPTQQEQI
LDWFHLMENLHKVGGSLKRLQQAETFLWRGKIDEAIDLFCGLSKPQAKRFCQYLRDHRERIPNYGYYQAEGIPIGSGAVESLVKQIDRRTKISGAQWQEK
HIPKVLAHRCAYLNRQLDSIFLLKK
VHRQTFSPPSLTQGVTQLSLDGGNIRLITPKGEPCHWRGYKAVRINGDRVGLAYYQDHTSLCLAVNRFRFAARVYCLGDGHVGIWKLYRQMRLPTQQEQI
LDWFHLMENLHKVGGSLKRLQQAETFLWRGKIDEAIDLFCGLSKPQAKRFCQYLRDHRERIPNYGYYQAEGIPIGSGAVESLVKQIDRRTKISGAQWQEK
HIPKVLAHRCAYLNRQLDSIFLLKK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1091 bp | 363 aa | 65 | 1155 | + | Yes |
Chemistry : DDE
ORF sequence :
MTPEEEQEFNACVTRISELLYQDSQSQSLPMKTLAEIECTVRTQLQTHVSPQMGLFFIDQVSPPDVGEYRRTLKSILGKLNLTRTQATALKVKPNSQLSP
YLEMCALRISANVSYAHASEDLAVLTGITVSSKTQQRLVHRQTFSPPSLTQGVTQLSLDGGNIRLITPKGEPCHWRGYKAVRINGDRVGLAYYQDHTSLC
LAVNRFRFAARVYCLGDGHVGIWKLYRQMRLPTQQEQILDWFHLMENLHKVGGSLKRLQQAETFLWRGKIDEAIDLFCGLSKPQAKRFCQYLRDHRERIP
NYGYYQAEGIPIGSGAVESLVKQIDRRTKISGAQWQEKHIPKVLAHRCAYLNRQLDSIFLLKK
YLEMCALRISANVSYAHASEDLAVLTGITVSSKTQQRLVHRQTFSPPSLTQGVTQLSLDGGNIRLITPKGEPCHWRGYKAVRINGDRVGLAYYQDHTSLC
LAVNRFRFAARVYCLGDGHVGIWKLYRQMRLPTQQEQILDWFHLMENLHKVGGSLKRLQQAETFLWRGKIDEAIDLFCGLSKPQAKRFCQYLRDHRERIP
NYGYYQAEGIPIGSGAVESLVKQIDRRTKISGAQWQEKHIPKVLAHRCAYLNRQLDSIFLLKK
Blast result :
Comments
ISAcas2 is 78% aa similar to ISCysp24.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Miller,S.R., Wood,A.M., Blankenship,R.E., Kim,M. and Ferriera,S.(2011)Genome Biol Evol 3, 601-613.
2] Miller,S.R., Wood,A.M., Blankenship,R.E., Kim,M. and Ferriera,S.(2011)Genome Biol Evol 3, 601-613.