ISAtsp10

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_CAFN01000002 ND Arthrospira sp.
Arthrospira sp. PCC 8005
DNA section
IS Length : 1574 bp

Ends


IR Length : 19/20

IRL : GGGAGCATCTCATTTTGGCAGCTAATACTTCTTAGAGGCCACCCCAAGCC
IRR : GGGAGCATCTCAATTTGGCAAAAATATTATCTTTCTAGGGTCGGATTATG

Insertion site


Left flankDirect repeatRight flankDR Length
TGTTGAGTGGCAAATTTTCTTGAGTATT8

DNA sequence

GGGAGCATCTCATTTTGGCAGCTAATACTTCTTAGAGGCCACCCCAAGCCTATAACTTTTCAAAAAATTGCTGATTGCCAACTTTAAAATTACAGAAAGC
TAAACGGTACTAAGTTGTTCACCGTCTAAATTGGAGGCAACAAGGGAAATCATGAAACTCAACTGGGTGCGGCGTTGCACAGTATGAATGAGCTAGAAGC
ATCCAATTTAAATACTCCATCGCTGCTCTTTTGATTTGATGTGATAAAATTTTATCGAGTTGAATTTAATTGAATCTGATGAACTTGACTCCTGAACAGT
TAGAACGCCTTGACGCCTGTATCCAAGAAATTGGTGAAATTATGTACAGCCAGATGGACTCAGGTAAGCTAAAAGACATGGAAATGTTGGAAACCATGAC
ACGGCAACAGATATTGGATTATATAGGGCCGAAACTTTTATTTTTTTTTATTTCTCAAGTTACCGGAACAACTCACGGAAAAAAACGGAAACTTAAAACT
TGTTTAGGTTCCGTAACTTTAACCAGTAAACAAGCCCAGATTATGGGAATAAATTCCTATGCTCGTCTTAGCCCCCTTTTGTTAAAATGTAGTTGGTTAG
TCAGTGCTAAGTCTTCTTATGAAAGGGGAGAAAAAGACATAAAAGTATTGACAGGTGTCCAAGTTTCTCATAGTACCTTACAACGTCAAGTTAATGAATC
AGACTTTGATTTTCCTTCAGCGAAACAGCCTGTAAGTGAAGTCTGTATTGATGGAGGTAAAGTCAGATTGCGTACTCCAGAAAAAGGCGTTTCTTGTATC
TGGAAAGATTATAAAGTAGGGCGAATGCAAGGAATTTACTATGGGGCAACCTTTCAAGACCCTCAAACTTTAACGGATTGGATTAATAGTCAAAAAACGA
CGAATCCACTCATCTATTTGGGAGATGGGCATGACGGAATTTGGAAGCTTTTTAAAGAAATTGGTAGCCCCGAACAACGTCAGGAAATTTTAGATTGGTT
TCATCTTAAAGAAAATCTTTTTAAAGTTAGAGCTTCAAAGAAGAACCTGAAAACAATCGAAGCGTTACTCTGGAAGGGCCAAGTTATAGAGGCTAAAGCC
TCATTGCTCAAGTATAAAAGTCACCAAGCCTCTAAATTTAGGGCGTATTTAACCAAGCATGAATCCCGAATTATTAATTATGAGCAAGCTCAAAAAGAAC
AAATTTGTTCCATTGGCTCGGGGGCTGTAGAGTCAGGGGTTAAGCAAATTGACAAACGATTAAAATTAGCTGGAGCTCAATGGAATTCGGAAAATATTAC
GAAGATTTTAGGGTTACGCTGTGCTTATCTTAATGGGAGCCTAGATGCGTACATAATGTGACCAGAATTGACCGTTAGTTTTTATTTTTTTTACAGTTTA
CCACTTAATATTTTATCCAATTTATCTGACTACCATATGGGAAGTTAGGTCGTTTTATTCTGCCATAAATTTGCTCCAAGATTTGAGCAATATTTTTCTC
TGCAACCCCGGTGAAGGATCCTCCCATAATCCGACCCTAGAAAGATAATATTTTTGCCAAATTGAGATGCTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1083 bp360 aa2791361+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNLTPEQLERLDACIQEIGEIMYSQMDSGKLKDMEMLETMTRQQILDYIGPKLLFFFISQVTGTTHGKKRKLKTCLGSVTLTSKQAQIMGINSYARLSPL
LLKCSWLVSAKSSYERGEKDIKVLTGVQVSHSTLQRQVNESDFDFPSAKQPVSEVCIDGGKVRLRTPEKGVSCIWKDYKVGRMQGIYYGATFQDPQTLTD
WINSQKTTNPLIYLGDGHDGIWKLFKEIGSPEQRQEILDWFHLKENLFKVRASKKNLKTIEALLWKGQVIEAKASLLKYKSHQASKFRAYLTKHESRIIN
YEQAQKEQICSIGSGAVESGVKQIDKRLKLAGAQWNSENITKILGLRCAYLNGSLDAYIM

 

Blast result :
Comments
ISAtsp10 is 69% aa similar to ISNpu14.
References
1] ISfinder annotation (2013)
2] Genoscope,C.E.A. (2011) Direct submission GenBank.