ISAcas1

  • Family ISKra4
  • Group ISAzba1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AFEJ01000343 ND Acaryochloris sp.
Acaryochloris sp. CCMEE 5410
DNA section
IS Length : 1467 bp

Ends


IR Length : 20/22

IRL : GTAAGCGTTAATATCCTATATTTTCTATATTTTTCATGTTACTCTATCTC
IRR : GAGAGCGTTAATATCCTATATTGAAAAGCTAATTTTTAAGCAGCTTTATC

Insertion site


Left flankDirect repeatRight flankDR Length
ATGGATCATAATGTTTCATG0

DNA sequence

GTAAGCGTTAATATCCTATATTTTCTATATTTTTCATGTTACTCTATCTCTTGCAGGGATTTGAAAGTAACGGTGAGAGATGGACTACGAATTTAGAGTG
ATTGTGGAGAAAGTTTCAGTTACCACTCAGGAAGTTGTTAAGCGAGACACTCTAAAAATTTATGATATCAAGCAACCAGAATCCATATTAGATCTAGGAT
TGCGTCACGAAGAACAAATCTCGCTGCTTTCCAAAGTTCAAAGTGCTCTGTTAGCTGAACAATCTACCCTCATCGATCCTGGATTCAAGCAGTGTATCAA
ATGCGGGGAGAAAATAGGCAAGCTTGGTTTTCAATCCTCTACCTTCCATGCTGTCTTCAGCGATCATAAACTCCGTATCCAAAAACATCGGTGCAAGAAT
CCAGATTGTCGTTGGCAAAGCTCTCCCACAACAATCAGTGTCTTTGGCACAGATACTCATCCAGATTTAGTGAAACTGCAATGTGAGAATGGTGCCTTAC
ATAGCTATCGAGAAGCCGAAGACAATTTGGAAAGACAAAATGCCCAACGTCGTACTGTAAATAACCACGTCCAGATCAAAAAGATCACTGACCAAATTGG
CGAGCGCTTATCTGAGGAGAACAAAACGCCTCCCATCAGTGATGAATGTGCTCCAGCTGCCCCAGAGCTGATTGTACAAGTTGATGGGGGTCACATTCCG
ACCAAAGATAAAGACAAACGCAGTTTTGAAGCTCTATCTGGCATCGTCTATCGTCCGAGTGCGATTGAAGTGGTGGATCAACACCATCGCCAGATTACGG
ATAAGACTTGTGTTGTTTCCGCCTTAGACGATGAGTTGCAAACCATCAAAACCTACCTGCATCATGCAGCCCTTAAACAAGGAATGACCCAAGAGACCAA
GGTTACCGCTTTGGCCGATGGCGCACACAACTGTTGGTCCGTTATCTTAGCTCTGGAGCCTCACTGCAAGTCCTTAGAGTTAGTCTTGGACTGGTTTCAC
ATTGGCAAGAAATTCAAGAATGTCAGCAATGCGCTGGGTGAGACCTTCACAAAGTCTCTCGACAGTACAAAGTGGAGTTTATGGCATGGCAAGGTAGATG
ACGCTCTGCGGAAGATTGCTCTGCTTCGAGATAATATCTCAGATGACAAGAAGCAAACAAAGCTCACAAGCTTGCACGATTATTTGAAGAATAACCGTGA
TTATCTTGTCAATTATGATGAACGAGAGAAAGCAGGGTTAGCCTATACTAGCCAGGTCGCAGAATCTCATATTGACACCATCATTAATGCTCGACACAAA
AAGAAGCAGAAGATGCAATGGACTCGGGATGGTGCCCACAATGTCTTGCAAATCAGAGCCAGTATGATCAGTAATGAATGGGATGAAAAGTGGCTTGATT
TGGTACTGCCTGAAGAAGATAAAGCTGCTTAAAAATTAGCTTTTCAATATAGGATATTAACGCTCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1353 bp450 aa801432+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDYEFRVIVEKVSVTTQEVVKRDTLKIYDIKQPESILDLGLRHEEQISLLSKVQSALLAEQSTLIDPGFKQCIKCGEKIGKLGFQSSTFHAVFSDHKLRI
QKHRCKNPDCRWQSSPTTISVFGTDTHPDLVKLQCENGALHSYREAEDNLERQNAQRRTVNNHVQIKKITDQIGERLSEENKTPPISDECAPAAPELIVQ
VDGGHIPTKDKDKRSFEALSGIVYRPSAIEVVDQHHRQITDKTCVVSALDDELQTIKTYLHHAALKQGMTQETKVTALADGAHNCWSVILALEPHCKSLE
LVLDWFHIGKKFKNVSNALGETFTKSLDSTKWSLWHGKVDDALRKIALLRDNISDDKKQTKLTSLHDYLKNNRDYLVNYDEREKAGLAYTSQVAESHIDT
IINARHKKKQKMQWTRDGAHNVLQIRASMISNEWDEKWLDLVLPEEDKAA

 

Blast result :
Comments
ISAcas1 is 44% aa similar to ISSusp4.
References
1] ISfinder annotation (2013)
2] Miller,S., Kim,M., Montgomery,R., Johnson,J., Ferriera,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Nelson,K.N. and Venter,J.C. (2010) Direct submission GenBank.