ISAra2
- Family ISKra4
- Group ISAzba1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011987 | ND | Agrobacterium radiobacter | Agrobacterium radiobacter K84 plasmid pAtK84c |
DNA section
IS Length : 2535 bp
Ends
IR Length : 24
IRL : GAGAGCATGAGGAATTGTGTGGCAGTGCCTGATCTAGCTTGAATGGGCTA
IRR : GAGAGCATGAGGAATTGTGTGGCAATCAGGCCGCGGCGGAATTGTTGCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGGTAAAAC | CCAATGGCGC | 0 |
DNA sequence
GAGAGCATGAGGAATTGTGTGGCAGTGCCTGATCTAGCTTGAATGGGCTATGGGGGTGGCGATGATTTTCGCGGAGGCCCATGGTTTTGAAATCAACAAC
CAAGAGACGTCAGTCGGCGACAAAACGTCCATTGAAGCCGGATGAGTCTCTCATGATCCTGCAATTCCGGATCTTTCTGAAAGACGTGAGCCCCATGGTC
TGGCGGCGGGTGCACGTGCCCTCGACCATGACGCTACGCGAGTTTCACGGCGTGCTTCAGGTCGTGATGGGCTGGGAGAGCATTCACCTTTATGAGTTTG
TCATTCATACGGTGCGTTACGGCTCCTGGGAAACGGCCGCTAACTCGCCGAAGATCGCGCTTGACGATCTGAAACTGCGCAAGGGTAGCCGTTTTCTGTA
TGAGTACGACCTCAACATCCCGTGGCAGCACGAAATCCGGCTGGAAGAGCGCGTGAGCGTCAATGCGAAAACTCGCTATCCGAATTGCTCCGGAGGGGAC
GGCAATTGCCCGCAGGAGGATTGCGGTGGCCCGGAGGGCTGGATGTGGCGACAGGACAACGCCTTCGGATACGAAATGGTCGATGATCTGGCTACGACGG
TGGCGCTTTTCCAGGAAATCAGCGAAACGAAGTCCTTCGCAGTACTGGAGGACCCGGGCCGGTTGGAAGAATTCCGTGAAACGCTTGAACGGATTGAGCA
GTGCGCTTCTTGGAATGGCCAGCCATTCGAACGCCGGAAAGTGAATGAGCGCCTGCGGCAGGGCGACCATCTTGTACTCATGCATCAGCAGATGTGAGCC
GCCTTACGCGGCGATTTTCCGAGAGAAGATTGGCAGCCGGAAGCGCCGTCTTGTCACCAACCTGCGCACGGGCGCAGGTGATCGCCGCCTTGCATTGCGG
GTAATCGATCCCTCCTGATCGGGATGCTTGCGATAGCACTTGCGGCGCGGTCTATCGAAGTCATGGCGGCTTACCAAAGAATTTTCGGATTGCTAACTCA
TGCGGAATATCACCGTCCCCGACAAGACTGGAGAACGGCAAAAGCATGTTGCAGGCGCAATCGATTTTCCATTCCGGTCATTATGGAAAACGCGTCACGG
AAAAATGATATGCGGGCACGGCTAGTACTGGAAATAGTGGGTGACAACGGCGAGACCATCGCGACCGAGACGGTTGCCGATCTGGTAAAAGTGACGAAAG
GGGCCGAGGATCTCGGTTTGTCGTTGTCGGAGGCGAAGACCCTTCTCGCGAAGCTTCAGCAGAAGATGGTGGAGACACAGGTCGATGTGTGGCTCCGGCA
GTACCATGAGGGTGATGGGCGTGCCTTGCGGCACAAGGGATATTACCCTGTCACATTCCACACACTGTTCGGCGATGTTCGCCTGAAGAGTCCGCGATAC
TGCCTGCCGCGCAGGGACAGAAGCAACGGCCCGGTTACAGTATCGCCTTTGAGGCAATTGATCCCCGATCATGTCGCGCCCGAGCGTCTTTATCTCGAAA
CGCGCTGGGCTTCGCTGGTGCCCTACGCAGCGGCGGCAGAGTTGTTGGCGGATATGCTACCGATCGACTGCGGATCCAACGCAACGACGGTGCGGCAACA
TGCGCTGCGAACGGCCAGCCGGATCGAACAGGAACTGACGGAAGAGCGGGTTTCCTTCATGCAGAATTCCTGTCTACGCGACTGGATGGACTTGCCCATT
CCTGATGGGCGGATCGTCATAGGCTTGGATGGCGGATATGTTCGCGATCGCGACGACCGCAAGAGAAACTTCGAGCTGATCGTCGGCCGCTCGCTGCCCG
AAGATGGCGATCCACGTTACATCGGCTTTGTTCATGGGTACGATCGCAAACCGCAGCGCCGGATTCTCGACCATCTCAAAAAGCAGGGAGTGCAGGCCAA
CCAGGATATCACCTTCATCACAGATGGTGGCGAGGAAGTCCGCTCACTCGCGAAAATGATTGCACCGGCCAGCGAGCATGTGCTGGACTGGTTCCATATC
ACCATGCGGATCACCGTTCTTCGCCAGTTCGCACAAGGGCTTGAAAACCACGATGAACAGGCCGGGCTGAATATGCTTGACGCCCTCGAAAGGATCAAAT
GGTATCTCTGGCATGGCAACGGATACCGTGTGCGCGGCGAAATCAGCGATTTGTACTGCGACGCTGTAGCAATCGAAACAGCTTATCCGAACATACGGAA
ATTCCTGATGGCTATCGGCGAATTCCGTTCCTACATCGCCGCAAACGATGCCAGCCTGATCAACTACGGTGAGCGCTACCGGTCTGGCGAGCGGATATCT
TCGGCCCTCGTCGAAGCCACCGTGAACGCCGTCATCAGCAAGCGTTTCGCCAAAAAGCAGCAGATGCAATGGAGCCGGACCGGCGCGCATCTTTTACTAC
AAACCAGAACGCAGACCCTCGACGGCTCGCTGCGGTTAACCTTCAAGAAATGGTATCCAGGCATGGCAAACGACAATCACGAACCCGGCAACAATTCCGC
CGCGGCCTGATTGCCACACAATTCCTCATGCTCTC
CAAGAGACGTCAGTCGGCGACAAAACGTCCATTGAAGCCGGATGAGTCTCTCATGATCCTGCAATTCCGGATCTTTCTGAAAGACGTGAGCCCCATGGTC
TGGCGGCGGGTGCACGTGCCCTCGACCATGACGCTACGCGAGTTTCACGGCGTGCTTCAGGTCGTGATGGGCTGGGAGAGCATTCACCTTTATGAGTTTG
TCATTCATACGGTGCGTTACGGCTCCTGGGAAACGGCCGCTAACTCGCCGAAGATCGCGCTTGACGATCTGAAACTGCGCAAGGGTAGCCGTTTTCTGTA
TGAGTACGACCTCAACATCCCGTGGCAGCACGAAATCCGGCTGGAAGAGCGCGTGAGCGTCAATGCGAAAACTCGCTATCCGAATTGCTCCGGAGGGGAC
GGCAATTGCCCGCAGGAGGATTGCGGTGGCCCGGAGGGCTGGATGTGGCGACAGGACAACGCCTTCGGATACGAAATGGTCGATGATCTGGCTACGACGG
TGGCGCTTTTCCAGGAAATCAGCGAAACGAAGTCCTTCGCAGTACTGGAGGACCCGGGCCGGTTGGAAGAATTCCGTGAAACGCTTGAACGGATTGAGCA
GTGCGCTTCTTGGAATGGCCAGCCATTCGAACGCCGGAAAGTGAATGAGCGCCTGCGGCAGGGCGACCATCTTGTACTCATGCATCAGCAGATGTGAGCC
GCCTTACGCGGCGATTTTCCGAGAGAAGATTGGCAGCCGGAAGCGCCGTCTTGTCACCAACCTGCGCACGGGCGCAGGTGATCGCCGCCTTGCATTGCGG
GTAATCGATCCCTCCTGATCGGGATGCTTGCGATAGCACTTGCGGCGCGGTCTATCGAAGTCATGGCGGCTTACCAAAGAATTTTCGGATTGCTAACTCA
TGCGGAATATCACCGTCCCCGACAAGACTGGAGAACGGCAAAAGCATGTTGCAGGCGCAATCGATTTTCCATTCCGGTCATTATGGAAAACGCGTCACGG
AAAAATGATATGCGGGCACGGCTAGTACTGGAAATAGTGGGTGACAACGGCGAGACCATCGCGACCGAGACGGTTGCCGATCTGGTAAAAGTGACGAAAG
GGGCCGAGGATCTCGGTTTGTCGTTGTCGGAGGCGAAGACCCTTCTCGCGAAGCTTCAGCAGAAGATGGTGGAGACACAGGTCGATGTGTGGCTCCGGCA
GTACCATGAGGGTGATGGGCGTGCCTTGCGGCACAAGGGATATTACCCTGTCACATTCCACACACTGTTCGGCGATGTTCGCCTGAAGAGTCCGCGATAC
TGCCTGCCGCGCAGGGACAGAAGCAACGGCCCGGTTACAGTATCGCCTTTGAGGCAATTGATCCCCGATCATGTCGCGCCCGAGCGTCTTTATCTCGAAA
CGCGCTGGGCTTCGCTGGTGCCCTACGCAGCGGCGGCAGAGTTGTTGGCGGATATGCTACCGATCGACTGCGGATCCAACGCAACGACGGTGCGGCAACA
TGCGCTGCGAACGGCCAGCCGGATCGAACAGGAACTGACGGAAGAGCGGGTTTCCTTCATGCAGAATTCCTGTCTACGCGACTGGATGGACTTGCCCATT
CCTGATGGGCGGATCGTCATAGGCTTGGATGGCGGATATGTTCGCGATCGCGACGACCGCAAGAGAAACTTCGAGCTGATCGTCGGCCGCTCGCTGCCCG
AAGATGGCGATCCACGTTACATCGGCTTTGTTCATGGGTACGATCGCAAACCGCAGCGCCGGATTCTCGACCATCTCAAAAAGCAGGGAGTGCAGGCCAA
CCAGGATATCACCTTCATCACAGATGGTGGCGAGGAAGTCCGCTCACTCGCGAAAATGATTGCACCGGCCAGCGAGCATGTGCTGGACTGGTTCCATATC
ACCATGCGGATCACCGTTCTTCGCCAGTTCGCACAAGGGCTTGAAAACCACGATGAACAGGCCGGGCTGAATATGCTTGACGCCCTCGAAAGGATCAAAT
GGTATCTCTGGCATGGCAACGGATACCGTGTGCGCGGCGAAATCAGCGATTTGTACTGCGACGCTGTAGCAATCGAAACAGCTTATCCGAACATACGGAA
ATTCCTGATGGCTATCGGCGAATTCCGTTCCTACATCGCCGCAAACGATGCCAGCCTGATCAACTACGGTGAGCGCTACCGGTCTGGCGAGCGGATATCT
TCGGCCCTCGTCGAAGCCACCGTGAACGCCGTCATCAGCAAGCGTTTCGCCAAAAAGCAGCAGATGCAATGGAGCCGGACCGGCGCGCATCTTTTACTAC
AAACCAGAACGCAGACCCTCGACGGCTCGCTGCGGTTAACCTTCAAGAAATGGTATCCAGGCATGGCAAACGACAATCACGAACCCGGCAACAATTCCGC
CGCGGCCTGATTGCCACACAATTCCTCATGCTCTC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 645 bp | 214 aa | 153 | 797 | + | No |
Annotation : plasmid pRIA4b ORF-3 family proteinDescription :
ORF sequence :
MILQFRIFLKDVSPMVWRRVHVPSTMTLREFHGVLQVVMGWESIHLYEFVIHTVRYGSWETAANSPKIALDDLKLRKGSRFLYEYDLNIPWQHEIRLEER
VSVNAKTRYPNCSGGDGNCPQEDCGGPEGWMWRQDNAFGYEMVDDLATTVALFQEISETKSFAVLEDPGRLEEFRETLERIEQCASWNGQPFERRKVNER
LRQGDHLVLMHQQM
VSVNAKTRYPNCSGGDGNCPQEDCGGPEGWMWRQDNAFGYEMVDDLATTVALFQEISETKSFAVLEDPGRLEEFRETLERIEQCASWNGQPFERRKVNER
LRQGDHLVLMHQQM
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1446 bp | 466 aa | 1065 | 2510 | + | No |
Chemistry : DDE
ORF sequence :
MRARLVLEIVGDNGETIATETVADLVKVTKGAEDLGLSLSEAKTLLAKLQQKMVETQVDVWLRQYHEGDGRALRHKGYYPVTFHTLFGDVRLKSPRYCLP
RRDRSNGPVTVSPLRQLIPDHVAPERLYLETRWASLVPYAAAAELLADMLPIDCGSNATTVRQHALRTASRIEQELTEERVSFMQNSCLRDWMDLPIPDG
RIVIGLDGGYVRDRDDRKRNFELIVGRSLPEDGDPRYIGFVHGYDRKPQRRILDHLKKQGVQANQDITFITDGGEEVRSLAKMIAPASEHVLDWFHITMR
ITVLRQFAQGLENHDEQAGLNMLDALERIKWYLWHGNGYRVRGEISDLYCDAVAIETAYPNIRKFLMAIGEFRSYIAANDASLINYGERYRSGERISSAL
VEATVNAVISKRFAKKQQMQWSRTGAHLLLQTRTQTLDGSLRLTFKKWYPGMANDNHEPGNNSAAA
RRDRSNGPVTVSPLRQLIPDHVAPERLYLETRWASLVPYAAAAELLADMLPIDCGSNATTVRQHALRTASRIEQELTEERVSFMQNSCLRDWMDLPIPDG
RIVIGLDGGYVRDRDDRKRNFELIVGRSLPEDGDPRYIGFVHGYDRKPQRRILDHLKKQGVQANQDITFITDGGEEVRSLAKMIAPASEHVLDWFHITMR
ITVLRQFAQGLENHDEQAGLNMLDALERIKWYLWHGNGYRVRGEISDLYCDAVAIETAYPNIRKFLMAIGEFRSYIAANDASLINYGERYRSGERISSAL
VEATVNAVISKRFAKKQQMQWSRTGAHLLLQTRTQTLDGSLRLTFKKWYPGMANDNHEPGNNSAAA
Blast result :
Comments
ISAra2 is 73% aa (ORFB : the transposase) similar to ISNov1.
ORFA is a passenger gene annotated as plasmid pRiA4b ORF-3-like protein.
ORFA is a passenger gene annotated as plasmid pRiA4b ORF-3-like protein.
References
1] ISfinder annotation (2013)
2] Setubal,J., Wood,D., Slater,S., Nester,E., Farrand,S., Goldman,B., Burr,T., Goodner,B., Otten,L., Dickerman,A., Almeida,N., Sun,J., Jhaveri,J., Banta,L. and Houmiel,K. (2009) J. Bacteriol. 191(8):2501-11.
2] Setubal,J., Wood,D., Slater,S., Nester,E., Farrand,S., Goldman,B., Burr,T., Goodner,B., Otten,L., Dickerman,A., Almeida,N., Sun,J., Jhaveri,J., Banta,L. and Houmiel,K. (2009) J. Bacteriol. 191(8):2501-11.