ISfinder Mirror Site

ISUnCu7

Family ISKra4
Group ISAzba1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AB266113	ND	uncultured bacterium	uncultured bacterium

DNA section

IS Length : 2273 bp

Ends

IR Length : 22

IRL : GGAGTACCAAAATCTGTGGGGTGTTGGTAGCCGGTGGGCTGAAGGCTGGG
IRR : GGAGTACCAAAATCTGTGGGGTATCAAGCGGCCATTGCGGTTTCTGACTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGGGCAGGGG		CCTACAGGTG	0

DNA sequence

GGAGTACCAAAATCTGTGGGGTGTTGGTAGCCGGTGGGCTGAAGGCTGGGTAGGCTTTTGTCGTTGAAAATGTCGAACCGGATATGCCATCCACATCCCC
ATCATTGCCGACGTCCACGGGGTCCATCCTCCAGCTCAAAATCCGTCTGCTTGGTCTGAGCCCGATGGTGTGGCGACGTGTCCTGGTTCCGGAGAGCTAT
TCGTTGCGGGAGCTTCACGGCGCAATCCAGGTGGCCATGGGCTGGGAGAGCTTGCATCTCTTTGAGTTCAGGATACGCGCGGTTCGTTACGGTTCGTCGG
ATATTTGCACTGAACCGCCTGACAAGACGCTCGACAGCTTTGGGTTCCGCAAGAACGCCAAGTTTGCCTACGTCTATGACATGGGCGACTGGTGGGAGCA
TGAAGTCCGCGTCGAAGACCGGGAAGTGGCGAAGGAACGCGGGCGCTATCCGGTCTGCGTCGGCGGCGCGGGCGCCTGCCCGCCGGAGGATTGTGGCGGC
CCGGACAGGTATCTCGCGCGGCGTGATGACGCGCTTGGCCCGGACACCATGGATGACCTGGCAACCATGGCGGATTTTGTCGAGCAGGTTGTTTTCAATG
GCAACCGTGCCATGCTCGATGATGATGAAGCACGTCAGGCGGTCGAACGCGCAATTGACCGGTCCCGGTCTCGCGCGCCGTTTCTCGCCTGCCGGTTTTC
TCTGCGGGACGTGAACACCGGCTTTCGCCAGGATGAGCACCGGCGGCTGATGTATCAATGGCTCATATGAGTATCGCCGCTCGGGAGCTGAAGGCATGGC
TCGTGCTTCTTCTCCGTGCATTCTGCAAAACCGGGGTTAAAGGCAAGCAATGAAGATTGAGATCAAGCTGCGGATCGCCGCAGCGGACGGCAGCATTTGC
GATGAAGAGATTTTGAGATTCGACAAACCCCATGACCAGCTTGAGGCAATCGGATTGTCGCTCGCCGAGGCCAAGGATCTGCTTGGCCGCCTGCAGGAGC
ACATCGTTGCGGCGCAAGCGGCAACCTTTGCCGCCGAACGGCGCTGTTGCCAACAATGCGGCCGAGCACAGACGAGCAAGGGCAGCACATCGATCCGCTT
CCGAACGCCATTTGGCGACGTGCCGGTTTCCAGTCCGCGGCTTCACCGCTGCGCTTGCGATGCCGGTCAGACCCGGACCTTCAGCCCGCTGACGACGCTG
TTTTCCGAACACGTTGCGCCCGAACTGCTCTACCTTGAGACCAAATGGGCCTCGCTGGTCTCCTTCGGCGTCACCGTCGATCTTCTGAAGGATGTCTTGC
CGATCGGTTCTACGCTGAACGCCGAAACGGTCCGCAACCATCTTCATCGCATAGCCGCACGCGCCGAAGCGGAGCTCGGCGACGAGCGCCCCTCCTTCAT
CGAAGGCTTTCCACGGATGTGGGCGCAGTTGCCCATCCCCGAAGGCCCGATCGTCGTCGGTGTGGATGGTGGATATGTCCGCGCCCGCGACGGCAGGCAG
AGCCATTTCGAGGTCATGGTCGGAAAATCGATTCCGGAAGATCGGAAGAACCGGTATTTCGGCCTGGTCCAGAGCCATGACGACAAGCCCCGCCGCCGCT
TGCGAGAGGTGCTGCGCGAGCAAGGCTTTCAGATGAACCAGGACATCACCTTCCTCACCGACGGTGGCGATAGCGTCCGCAACATGGCGGTTGCCATGTC
TCCATGCGCCGAGCATGTGCTCGACTGGTTTCATATCACCATGCGGCTGACCGTTCTCGGCCAGTACGCCAAGGGGCTGGCGCATCATAATCCAAGCGAA
GCCGAAGACACCGCCCGCGAGTTGAAGCGCATCAAGGGCTATCTGTGGAACGGCAACCGCCGCCAGGCGCTGCCCTGCATCGAGTGGCTCATCGATGATC
TCGATGCGGTCGAAACCGATTATCCCAGCATGAAGGCATTCCGGAAGGCGGCGGGAGAATTCAACACCTACATCGCCAACAACGCCGGCATGATCCCGAA
CTACGCCGAGCGCCGCCGCTATGGCGAACGGGTTTCGACGGCGTTCGTGGAGTCGACCGTCAATGTCGTGGTCGGCAAGCGCTTCTCAAAGCTTCAGCAG
ATGCGATGGTCGAAGGAAGGGGCACATCTCATGCTGCAGACCCGAACCCGCGCGCTCGACGGGACGCTGCGCAGAAAGTTCGAGCAGTGGTATCCCGGAC
TGGCCAGCAACAATTCCGCCCCCCAGTCAGAAACCGCAATGGCCGCTTGATACCCCACAGATTTTGGTACTCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
687 bp	228 aa	84	770	+	No

ORF function : Passenger Gene
Annotation : plasmid pRIA4b ORF-3 family proteinDescription :

ORF sequence :

MPSTSPSLPTSTGSILQLKIRLLGLSPMVWRRVLVPESYSLRELHGAIQVAMGWESLHLFEFRIRAVRYGSSDICTEPPDKTLDSFGFRKNAKFAYVYDM
GDWWEHEVRVEDREVAKERGRYPVCVGGAGACPPEDCGGPDRYLARRDDALGPDTMDDLATMADFVEQVVFNGNRAMLDDDEARQAVERAIDRSRSRAPF
LACRFSLRDVNTGFRQDEHRRLMYQWLI

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1401 bp	466 aa	850	2250	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKIEIKLRIAAADGSICDEEILRFDKPHDQLEAIGLSLAEAKDLLGRLQEHIVAAQAATFAAERRCCQQCGRAQTSKGSTSIRFRTPFGDVPVSSPRLHR
CACDAGQTRTFSPLTTLFSEHVAPELLYLETKWASLVSFGVTVDLLKDVLPIGSTLNAETVRNHLHRIAARAEAELGDERPSFIEGFPRMWAQLPIPEGP
IVVGVDGGYVRARDGRQSHFEVMVGKSIPEDRKNRYFGLVQSHDDKPRRRLREVLREQGFQMNQDITFLTDGGDSVRNMAVAMSPCAEHVLDWFHITMRL
TVLGQYAKGLAHHNPSEAEDTARELKRIKGYLWNGNRRQALPCIEWLIDDLDAVETDYPSMKAFRKAAGEFNTYIANNAGMIPNYAERRRYGERVSTAFV
ESTVNVVVGKRFSKLQQMRWSKEGAHLMLQTRTRALDGTLRRKFEQWYPGLASNNSAPQSETAMAA

Blast result :

Comments

ISUnCu7 is 73% aa (ORFB : the transposase) similar to ISAzs26.
ORFA is a passenger gene annotated as hypothetical protein (plasmid pRiA4b ORF-3 family protein ).

References

1] ISfinder annotation (2013)
2] Suenaga,H., Ohnuki,T. and Miyazaki,K. (2007) Environ. Microbiol. 9 (9), 2289-2297.
3] Suenaga,H., Koyama,Y., Miyakoshi,M., Miyazaki,R., Yano,H., Sota,M., Ohtsubo,Y., Tsuda,M. and Miyazaki,K.(2009) ISME J 3 (12), 1335-1348.