IS5Sa
- Family IS5
- Group IS1031
Isoform Synonym(s) IS5Sa1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U38799 | ND | Synechocystis sp. | Synechocystis sp. PCC6803 |
DNA section
IS Length : 871 bp
Ends
IR Length : 15/17
IRL : GAGCGTGTTTGAAAAGTAGGGTGAAGAATGGAAAATGGTGAAAAGAATAA
IRR : GAAAGTGTTTGAAAAGTTTTAAGCAAGCCTTTGAAGCATGAGATGAACCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | TAA | NNNNNNNNNN | 3 |
DNA sequence
GAGCGTGTTTGAAAAGTAGGGTGAAGAATGGAAAATGGTGAAAAGAATAAAATTTTCACCATAATTAATGATAATTCGAGAATTTTACAGCACGGACATC
ACTGATAGCCAGTGGCAATTGGTAGAACCCCATTTACCCAAAGCAAAAACTGGGGGAAGAAAACGGAAAACTAGTCTGCGGGAGATACTCAATGCCATTT
TTTACATGCTCAGAACGGGGTGTGCCTGGCGACTTCTACCCCATGATTTCCCTAAATGGCGAACGGTCTATGGTTATTTCCAGCAATGGCACGAAGATGG
TACCTGGAGAAAATTAAACCGTATCTTCCGTGAGAAAGTTCGGCTCAAAGCTGGAAGAAATACTCACCCTAGTGCCGGTTGTTTAGATTCACAGTCACTT
AAAAAGGCTGGAACTGGCCAAGAAACTGGCTATGACGGCGGAAAAAAGGTCAATGGTCGTAAACGTACAATCCTCGTGGATACCATGGGATTACTTCTCG
ATGTTGTCGTCCATGGTGCCCATCGCTCCGACCATCAGGGTCTGATTCTACTTGCTACCTGGTTTGCTCCCCTTTGGCAGTGCCTGCAAGTTATATGGAC
TGACAGCACCTTTGGCGGAAAGGATTTTATCTATTGGGTGGAGCATACTTTTGGTTGGAATTTGAATGTCGTCAGCAAAAAGCAGGGACAAAAAGGTTTT
GAAGTCTTACCCCGCCGTTGGGTAGTCGAGCGCACCTTTGCTTGGTTTGGTCGCTATCGTCGTTTAAGCAAGGATTATGAATATTTACCTACCACCAGTG
AGACCATGCTCTATGTTGCCATGGTTCATCTCATGCTTCAAAGGCTTGCTTAAAACTTTTCAAACACTTTC
ACTGATAGCCAGTGGCAATTGGTAGAACCCCATTTACCCAAAGCAAAAACTGGGGGAAGAAAACGGAAAACTAGTCTGCGGGAGATACTCAATGCCATTT
TTTACATGCTCAGAACGGGGTGTGCCTGGCGACTTCTACCCCATGATTTCCCTAAATGGCGAACGGTCTATGGTTATTTCCAGCAATGGCACGAAGATGG
TACCTGGAGAAAATTAAACCGTATCTTCCGTGAGAAAGTTCGGCTCAAAGCTGGAAGAAATACTCACCCTAGTGCCGGTTGTTTAGATTCACAGTCACTT
AAAAAGGCTGGAACTGGCCAAGAAACTGGCTATGACGGCGGAAAAAAGGTCAATGGTCGTAAACGTACAATCCTCGTGGATACCATGGGATTACTTCTCG
ATGTTGTCGTCCATGGTGCCCATCGCTCCGACCATCAGGGTCTGATTCTACTTGCTACCTGGTTTGCTCCCCTTTGGCAGTGCCTGCAAGTTATATGGAC
TGACAGCACCTTTGGCGGAAAGGATTTTATCTATTGGGTGGAGCATACTTTTGGTTGGAATTTGAATGTCGTCAGCAAAAAGCAGGGACAAAAAGGTTTT
GAAGTCTTACCCCGCCGTTGGGTAGTCGAGCGCACCTTTGCTTGGTTTGGTCGCTATCGTCGTTTAAGCAAGGATTATGAATATTTACCTACCACCAGTG
AGACCATGCTCTATGTTGCCATGGTTCATCTCATGCTTCAAAGGCTTGCTTAAAACTTTTCAAACACTTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 786 bp | 261 aa | 68 | 853 | + | No |
Chemistry : DDE
ORF sequence :
MIIREFYSTDITDSQWQLVEPHLPKAKTGGRKRKTSLREILNAIFYMLRTGCAWRLLPHDFPKWRTVYGYFQQWHEDGTWRKLNRIFREKVRLKAGRNTH
PSAGCLDSQSLKKAGTGQETGYDGGKKVNGRKRTILVDTMGLLLDVVVHGAHRSDHQGLILLATWFAPLWQCLQVIWTDSTFGGKDFIYWVEHTFGWNLN
VVSKKQGQKGFEVLPRRWVVERTFAWFGRYRRLSKDYEYLPTTSETMLYVAMVHLMLQRLA
PSAGCLDSQSLKKAGTGQETGYDGGKKVNGRKRTILVDTMGLLLDVVVHGAHRSDHQGLILLATWFAPLWQCLQVIWTDSTFGGKDFIYWVEHTFGWNLN
VVSKKQGQKGFEVLPRRWVVERTFAWFGRYRRLSKDYEYLPTTSETMLYVAMVHLMLQRLA
Blast result :
Comments
IS5Sa and ISRl2 transposases are 42 % identical.
There are 13 copies of IS5Sa in Synechocystis genome.
There are 13 copies of IS5Sa in Synechocystis genome.
References
1] Cassier-Chauvat, C., Poncelet, M., Villoing, S., and Chauvat F. "The nature of three insertion sequences from the cyanobacterium Synechocystis PCC6803 supports the hypothesis of trans-phylla and trans-kingdom gene transfers" (1996) EMBL Data Library, Rel. 47.