ISAfe13
- Family ISKra4
- Group ISAzba1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_015942 | ND | Acidithiobacillus ferrivorans | Acidithiobacillus ferrivorans SS3 |
DNA section
IS Length : 2868 bp
Ends
IR Length : 24
IRL : GAGAGTGAACCATTCCGGGGGCTGGCTCATCAGCCTGATTTTCGACGGTC
IRR : GAGAGTGAACCATTCCGGGGGCTGAAACCACTATGCCGCATCCTTTAGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCATAGACAGCGTCGC | GCTACGCACCACCATG | 0 |
DNA sequence
GAGAGTGAACCATTCCGGGGGCTGGCTCATCAGCCTGATTTTCGACGGTCAAACGGCCCGTGTTTTTCTGTGGGCACGCAGCAGCGTGGAAATGCTTACC
CCTGATTGCTTTGCCGCCGCTTGGTAGGTTACGCCCTGCTTGATCAGAGACAGGCCCTTGTCCACTTGCCGCGCTGTCGCGCAGGGCCGACCAAGGCGTT
TTCCTTTGGCGCGGGCATTGGCTAAGCCAGAGCGAACACGTTCCCGGATAAGCTCGCGTTCAAATTCAGCAACGGCTGATAGGAGGTGCGCCATCATGCG
GCCCATGGGTGTACTCATGTCGATATGACCCGGAACCACAAAGGCAACACCGAGCGTTTCCAATTCCGCCATGGTCAGGATGAGGTCCTGCACGCTACGA
CCCCAACGATCTAACGCTTGGACGAGGACCGCATCGATAGCGCGGGTGCGCGCCATTTGCAGGACTTCTTCACGTAACGGTCGCTTTTGTGATGCTCCGC
TGGCGGTTTCCTCGGTCTCTCGCACAATCTCCCAGCCGCGTGCTTTCGCGTAGTCACGTAATTCGCGCAGTTGCCGGTCGGTGGATTGGTCCGCAGTGGA
CACCCGGGTATAGATCGCCGCACGCATGGTCAAAAACCCCTTTGATTTTCGCGCCGTGCCCCCCAGTATTGGCGGGCCTTCCAGACGCCTCGTTTTCTAC
GGTCAAAATCAAGTAATTTTGACCGTCATGTATTAAGCTTAGATGACTATGGTAAACGAGACTAGCGCCGAGGTTTACCGGCTCCACGTATGGATTCGCC
AGATCACCCCGATGATTTGGCGGCGATTGCTCGTGCGCAGCGACAATACCATCACTGATCTGCATCATATCCTGCAAATCGCCTTCGGCTGGAGCGATGC
GCATTTGAATGGGTTTCACATCCATGGCCAGGACTACGGTGTGTATCATGATGGCGGTGTAAGCTTCTCTACCAACCCCAACCAAGTCCGACTGTGTGAT
TTCAAATTCCGTATCAATGAACGCTTCAGCTACGAGTACGACTTCGGCGACTGCTGGCAACACGTGGTGCGCGTTGAAGCGCATTTGAAGCCGGAAGACA
AAATGACTTATCCCCGTTGCATCGGCGGCCAGCGCCGGGCACCGCCGGAAGACTGTGGCGGCCCCTTGGCCTTCATGACGCGACGCGATGATATCTCGCT
GCAGGCTATGGATATCTTTGCGGACATCCTGGACGATCTGGAATGCCATAACGCCGAGGCGCTGGAAAACCATGTGGATAATATCAACGAGTTGCGTGAA
TGGCTGACGTTGGACCACTTTGATCGGCGCGCGGTCAACCGCCGACTGCGGCAGTACGCAGCTCACGATACGACATGGATGGATGTGGTAATCGCAAGGA
GCCTGTGATGAAAGTGCGCATACAGATGGTCATTGAAGCCGAAAATGGGGAGCCGGAGAAAGTCGAGGAAATCCTGCGATTGGAGCGCGGCACCCTCCGG
ACAGAGGATCTCGGACTGACCCTGGCAGAGGCAAAAAAGTTGCTGCGTGGCATGCAGCAGACCCTGCTCACGGAACAAGTCGCAGAATACCTCGCCACCT
TACAGGAATGCCCGGATTGTGGCGCACATCGAACCCGAAAAGGGCAGCATTACATCGTCTATCGGACGCTGTTCGGAAAATTCAACCTGATCAGCCCACG
TCTGTATGATTGCCCCTGCCAACACCATGGCCGACATAGCTCAAGCCCCCTGGCAGATATGTTGAGCACGCACTGTGCGCCGGAGCTGCAGTATCTGGAG
ACCAAATTTGCATCGCTGATGTCCTATGGGCTGAGTGTCCAGATGCTGACGGAAGTTTTGCCGATCGCCGAAGAAATCAATCCCACGTCGATACGGCGGC
GCCTGCATCAAGTTGCTGAGCGGATTGAGAGTGAACTGGGTGAGGAGCAGCCCCAGTTCATTGAAGGCTGCCCCAATGATTGGGCCCGGCAACCACCGCC
TGGGCCACCCATTATACTCGGTCTGGACGGTGGCTATGTACATGCGTCTGAGCAACGGTCAAGAACTGAAGGCTGGTTCGAGGTTATTACCGGCAAGAGT
GTTCAGGCCGAAGGTAATGCCAAGGTGTTTGCCTTCGTGAACAAATACGACACCAAGCCCCGGCGCCGGCTGCACGAGGTGCTGAAATCGCAAGGCTTGC
AAATGAACCAGCAGGTCGTGTTTCTATCCGATGGTGGCGATACCGTGCGTGATTTGCAGCATCGCCTCAGTCCGGAATCGGAGCATTTGCTCGACTGGTT
TCACATCACCATGCGGTTGACGGTAATGCAGCAGATGATTGTGGGCATGACGGTGGAGCTGAAGCCCGACGAAAAATGCCCGGAAATTGCCGCCGTGCTG
GCCGATCTGGAGAGCCATCGGGAAAGCCTCAAATGGAATCTCTGGCACGGCAATGTCCCGCACGCGCTGAAACGGATTGATAATCTGGAAGACGATCTGG
AGATGCTGGAGGAGAATCCGGCGAACAAGATGAAAATGCTCAAGGTGGTGCGAGAATTCCGCACCTATATTGACGCCAACCAGGCGTTTATCCCGAACTA
CGGCGACCGCTACCGGCATGACGAGATAATTTCCACGTCATTTGTCGAATCCACAGTGAACTATGTGGTCAGTAAACGATTCGTGAAAAAGCAGCAGATG
CGCTGGACCCAACGCGGTGCGCATCTGTTGCTGCAAACAAGGGTGCAGGTCCTGAACGATGACCTACGGAAAACGTTCATTCGCTGGTTCCCTGGGATGA
GCACGGGTGAGCAGGCGACGCTAAAGGATGCGGCATAGTGGTTTCAGCCCCCGGAATGGTTCACTCTC
CCTGATTGCTTTGCCGCCGCTTGGTAGGTTACGCCCTGCTTGATCAGAGACAGGCCCTTGTCCACTTGCCGCGCTGTCGCGCAGGGCCGACCAAGGCGTT
TTCCTTTGGCGCGGGCATTGGCTAAGCCAGAGCGAACACGTTCCCGGATAAGCTCGCGTTCAAATTCAGCAACGGCTGATAGGAGGTGCGCCATCATGCG
GCCCATGGGTGTACTCATGTCGATATGACCCGGAACCACAAAGGCAACACCGAGCGTTTCCAATTCCGCCATGGTCAGGATGAGGTCCTGCACGCTACGA
CCCCAACGATCTAACGCTTGGACGAGGACCGCATCGATAGCGCGGGTGCGCGCCATTTGCAGGACTTCTTCACGTAACGGTCGCTTTTGTGATGCTCCGC
TGGCGGTTTCCTCGGTCTCTCGCACAATCTCCCAGCCGCGTGCTTTCGCGTAGTCACGTAATTCGCGCAGTTGCCGGTCGGTGGATTGGTCCGCAGTGGA
CACCCGGGTATAGATCGCCGCACGCATGGTCAAAAACCCCTTTGATTTTCGCGCCGTGCCCCCCAGTATTGGCGGGCCTTCCAGACGCCTCGTTTTCTAC
GGTCAAAATCAAGTAATTTTGACCGTCATGTATTAAGCTTAGATGACTATGGTAAACGAGACTAGCGCCGAGGTTTACCGGCTCCACGTATGGATTCGCC
AGATCACCCCGATGATTTGGCGGCGATTGCTCGTGCGCAGCGACAATACCATCACTGATCTGCATCATATCCTGCAAATCGCCTTCGGCTGGAGCGATGC
GCATTTGAATGGGTTTCACATCCATGGCCAGGACTACGGTGTGTATCATGATGGCGGTGTAAGCTTCTCTACCAACCCCAACCAAGTCCGACTGTGTGAT
TTCAAATTCCGTATCAATGAACGCTTCAGCTACGAGTACGACTTCGGCGACTGCTGGCAACACGTGGTGCGCGTTGAAGCGCATTTGAAGCCGGAAGACA
AAATGACTTATCCCCGTTGCATCGGCGGCCAGCGCCGGGCACCGCCGGAAGACTGTGGCGGCCCCTTGGCCTTCATGACGCGACGCGATGATATCTCGCT
GCAGGCTATGGATATCTTTGCGGACATCCTGGACGATCTGGAATGCCATAACGCCGAGGCGCTGGAAAACCATGTGGATAATATCAACGAGTTGCGTGAA
TGGCTGACGTTGGACCACTTTGATCGGCGCGCGGTCAACCGCCGACTGCGGCAGTACGCAGCTCACGATACGACATGGATGGATGTGGTAATCGCAAGGA
GCCTGTGATGAAAGTGCGCATACAGATGGTCATTGAAGCCGAAAATGGGGAGCCGGAGAAAGTCGAGGAAATCCTGCGATTGGAGCGCGGCACCCTCCGG
ACAGAGGATCTCGGACTGACCCTGGCAGAGGCAAAAAAGTTGCTGCGTGGCATGCAGCAGACCCTGCTCACGGAACAAGTCGCAGAATACCTCGCCACCT
TACAGGAATGCCCGGATTGTGGCGCACATCGAACCCGAAAAGGGCAGCATTACATCGTCTATCGGACGCTGTTCGGAAAATTCAACCTGATCAGCCCACG
TCTGTATGATTGCCCCTGCCAACACCATGGCCGACATAGCTCAAGCCCCCTGGCAGATATGTTGAGCACGCACTGTGCGCCGGAGCTGCAGTATCTGGAG
ACCAAATTTGCATCGCTGATGTCCTATGGGCTGAGTGTCCAGATGCTGACGGAAGTTTTGCCGATCGCCGAAGAAATCAATCCCACGTCGATACGGCGGC
GCCTGCATCAAGTTGCTGAGCGGATTGAGAGTGAACTGGGTGAGGAGCAGCCCCAGTTCATTGAAGGCTGCCCCAATGATTGGGCCCGGCAACCACCGCC
TGGGCCACCCATTATACTCGGTCTGGACGGTGGCTATGTACATGCGTCTGAGCAACGGTCAAGAACTGAAGGCTGGTTCGAGGTTATTACCGGCAAGAGT
GTTCAGGCCGAAGGTAATGCCAAGGTGTTTGCCTTCGTGAACAAATACGACACCAAGCCCCGGCGCCGGCTGCACGAGGTGCTGAAATCGCAAGGCTTGC
AAATGAACCAGCAGGTCGTGTTTCTATCCGATGGTGGCGATACCGTGCGTGATTTGCAGCATCGCCTCAGTCCGGAATCGGAGCATTTGCTCGACTGGTT
TCACATCACCATGCGGTTGACGGTAATGCAGCAGATGATTGTGGGCATGACGGTGGAGCTGAAGCCCGACGAAAAATGCCCGGAAATTGCCGCCGTGCTG
GCCGATCTGGAGAGCCATCGGGAAAGCCTCAAATGGAATCTCTGGCACGGCAATGTCCCGCACGCGCTGAAACGGATTGATAATCTGGAAGACGATCTGG
AGATGCTGGAGGAGAATCCGGCGAACAAGATGAAAATGCTCAAGGTGGTGCGAGAATTCCGCACCTATATTGACGCCAACCAGGCGTTTATCCCGAACTA
CGGCGACCGCTACCGGCATGACGAGATAATTTCCACGTCATTTGTCGAATCCACAGTGAACTATGTGGTCAGTAAACGATTCGTGAAAAAGCAGCAGATG
CGCTGGACCCAACGCGGTGCGCATCTGTTGCTGCAAACAAGGGTGCAGGTCCTGAACGATGACCTACGGAAAACGTTCATTCGCTGGTTCCCTGGGATGA
GCACGGGTGAGCAGGCGACGCTAAAGGATGCGGCATAGTGGTTTCAGCCCCCGGAATGGTTCACTCTC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 579 bp | 192 aa | 627 | 49 | - | No |
Annotation : Serine recombinaseDescription :
ORF sequence :
MRAAIYTRVSTADQSTDRQLRELRDYAKARGWEIVRETEETASGASQKRPLREEVLQMARTRAIDAVLVQALDRWGRSVQDLILTMAELETLGVAFVVPG
HIDMSTPMGRMMAHLLSAVAEFERELIRERVRSGLANARAKGKRLGRPCATARQVDKGLSLIKQGVTYQAAAKQSGVSISTLLRAHRKTRAV
HIDMSTPMGRMMAHLLSAVAEFERELIRERVRSGLANARAKGKRLGRPCATARQVDKGLSLIKQGVTYQAAAKQSGVSISTLLRAHRKTRAV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 660 bp | 219 aa | 749 | 1408 | + | No |
Annotation : plasmid pRiA4b ORF-3 family proteinDescription :
ORF sequence :
MVNETSAEVYRLHVWIRQITPMIWRRLLVRSDNTITDLHHILQIAFGWSDAHLNGFHIHGQDYGVYHDGGVSFSTNPNQVRLCDFKFRINERFSYEYDFG
DCWQHVVRVEAHLKPEDKMTYPRCIGGQRRAPPEDCGGPLAFMTRRDDISLQAMDIFADILDDLECHNAEALENHVDNINELREWLTLDHFDRRAVNRRL
RQYAAHDTTWMDVVIARSL
DCWQHVVRVEAHLKPEDKMTYPRCIGGQRRAPPEDCGGPLAFMTRRDDISLQAMDIFADILDDLECHNAEALENHVDNINELREWLTLDHFDRRAVNRRL
RQYAAHDTTWMDVVIARSL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1431 bp | 476 aa | 1408 | 2838 | + | No |
Chemistry : DDE
ORF sequence :
MKVRIQMVIEAENGEPEKVEEILRLERGTLRTEDLGLTLAEAKKLLRGMQQTLLTEQVAEYLATLQECPDCGAHRTRKGQHYIVYRTLFGKFNLISPRLY
DCPCQHHGRHSSSPLADMLSTHCAPELQYLETKFASLMSYGLSVQMLTEVLPIAEEINPTSIRRRLHQVAERIESELGEEQPQFIEGCPNDWARQPPPGP
PIILGLDGGYVHASEQRSRTEGWFEVITGKSVQAEGNAKVFAFVNKYDTKPRRRLHEVLKSQGLQMNQQVVFLSDGGDTVRDLQHRLSPESEHLLDWFHI
TMRLTVMQQMIVGMTVELKPDEKCPEIAAVLADLESHRESLKWNLWHGNVPHALKRIDNLEDDLEMLEENPANKMKMLKVVREFRTYIDANQAFIPNYGD
RYRHDEIISTSFVESTVNYVVSKRFVKKQQMRWTQRGAHLLLQTRVQVLNDDLRKTFIRWFPGMSTGEQATLKDAA
DCPCQHHGRHSSSPLADMLSTHCAPELQYLETKFASLMSYGLSVQMLTEVLPIAEEINPTSIRRRLHQVAERIESELGEEQPQFIEGCPNDWARQPPPGP
PIILGLDGGYVHASEQRSRTEGWFEVITGKSVQAEGNAKVFAFVNKYDTKPRRRLHEVLKSQGLQMNQQVVFLSDGGDTVRDLQHRLSPESEHLLDWFHI
TMRLTVMQQMIVGMTVELKPDEKCPEIAAVLADLESHRESLKWNLWHGNVPHALKRIDNLEDDLEMLEENPANKMKMLKVVREFRTYIDANQAFIPNYGD
RYRHDEIISTSFVESTVNYVVSKRFVKKQQMRWTQRGAHLLLQTRVQVLNDDLRKTFIRWFPGMSTGEQATLKDAA
Blast result :
Comments
ISAfe13 is 64% aa (ORFC : the transposase) similar to ISAzs26.
ORFA and ORFB are passenger gene respectively annotated as resolvase domain-containing protein and plasmid pRiA4b ORF-3 family protein.
ORFA and ORFB are passenger gene respectively annotated as resolvase domain-containing protein and plasmid pRiA4b ORF-3 family protein.
References
1] ISfinder annotation (2013)
2] Liljeqvist,M., Valdes,J., Holmes,D.S. and Dopson,M. (2011) J. Bacteriol. 193 (16), 4304-4305.
2] Liljeqvist,M., Valdes,J., Holmes,D.S. and Dopson,M. (2011) J. Bacteriol. 193 (16), 4304-4305.