ISOni1
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_019730 | ND | Oscillatoria nigro-viridis | Oscillatoria nigro-viridis PCC 7112 plasmid pOSC7112.01 Oscillatoria nigro-viridis PCC 7112 Oscillatoria nigro-viridis PCC 7112 plasmid pOSC7112.02 Oscillatoria nigro-viridis PCC 7112 plasmid pOSC7112.03 |
DNA section
IS Length : 1180 bp
Ends
IR Length : 25/31
IRL : GGGTGCATCTCACTTTTGCGAATGTGTCAAAAAAGAGTTAAAATGAAAAA
IRR : GGGTGCATCTCACTTTGGCACATACATCAAATTGAAAGTTGACCGTTGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCAGAGTTC | GCGTCAGTG | TAGATACTAC | 9 |
| GTGGACGAAA | GCGGGTTG | AAACAAAATT | 8 |
| ATTTGATAGC | GAGCTGTGTC | 0 | |
| TATAAAATTA | AACACAGG | CTGCTATCAA | 8 |
| AAACAGCTCG | TAATTGCC | TGTGTCTTGA | 8 |
| TGACTTGTGA | GGAAACAG | CCTTGTATTT | 8 |
| TCTATTGTTT | GATGGCAA | TCGATCTAAA | 8 |
| TAACGACTTG | CGAAAGGC | ACTGCATTTA | 8 |
| TTACCAGCTC | AGCGTCGG/A | GCGTCGAGCG | 8 |
| GGTACTCGCA | GTGTAGAA | ATTCAATTGT | 8 |
| ATCTCGGTGG | ATCGCAGC | AGGTAACAGC | 8 |
| CCCTCTCTAC | GGTATCTT | GTTTATCCTC | 8 |
| TTTGAATGTC | TCTTGATG | TAGTCCACAG | 8 |
| AAGAAACCCG | GTTTCTTG | TTTCTAATTT | 8 |
| CAAATGCAAG | AGGCATCTTG | 0 | |
| GCTTGAGTGG | GTTGATAAC | CGCCTCGCA | 9 |
DNA sequence
GGGTGCATCTCACTTTTGCGAATGTGTCAAAAAAGAGTTAAAATGAAAAAGCTAGTAAAATCGTCAAATAAAGATGAGGTAAATAAAATGACACCCTCAG
ACCAACAACAACTAAAAGCCCACTTAAAAGCGGTAGCAAAAATTCTTTACAGAAATACAGAGCCAACCGAGTTAAAAAGTTTTGAAAGTATAGAAAAATC
AGTCCGTCAGAAAATGTTATCAGAAGTTGGTCCAGAAATAGGTAACTTTTTTTTTCAGCAGTATCAGGAATTCAAACAGGAAAACCCCGAAAAATAAAAT
CAATAATCGGCTCGCTCGACATTACAGATAATCAGGCAAAATATTTTGGCTTAAAAGCTTACAGTCAATTGAGTCCACTGATGGAAAATTGCTGTCTTTT
AATCACTGCTAACGAATCTTATCAAAGCGCCGAAAAAGATTTGGAAAAATTTACGGGGATCAAAATTTCTCACAGTACATTACAAAGATTAGTCAAGCGA
CAAGAATTTGAATTGCCTACATCTAAACAAGGAGTCAAAGAAATTACATTGGATGGCGGGAAAGTTAGACTACGCAACGAAACCAAGGGCGAGAGCTGTT
ACTGGAAAGACTATAAAGCCGTGTGTTTAGATAATGTTTATTCGGGAGCCTTTTTTCAAAATAATCAAGATTTAATTGATTGGACTAATAGCCAAAAATT
ACTACACCCTATGTATTGTCTAGGAGATGGTCATGCCGGGATTTGGAACATCTTTAAAGAAATTGGAGATAATGAACAAAGACAAGAAATTTTAGATTGG
TATCATCTCAAAGAAAATCTCTACAAGGTAGGGGGTTCAATAAAACGATTGAAATTAGCAGAAAATATGTTATGGCAAGGCAAAGTTGATGAAGTTAGAA
ATTTATTTAAAGACTTTAAAAGTCAAGCATTTAAAACGTTTTGCAATTATTTAGAGACCCATCGCTGCCGAATAGTGAATTACCAATACTACAAAGAAGA
GTCCATAAGTTCGATTGGTTCGGGAACAGTTGAATCAACAATTAAACGCATTGGATTAAGGGTGAAAATATCGGGAGCGCAATGGAATATTGAGAACGTT
TCCTCAATCCTGGCTCTTCGCTGTGCTTATCTCAACGGTCAACTTTCAATTTGATGTATGTGCCAAAGTGAGATGCACCC
ACCAACAACAACTAAAAGCCCACTTAAAAGCGGTAGCAAAAATTCTTTACAGAAATACAGAGCCAACCGAGTTAAAAAGTTTTGAAAGTATAGAAAAATC
AGTCCGTCAGAAAATGTTATCAGAAGTTGGTCCAGAAATAGGTAACTTTTTTTTTCAGCAGTATCAGGAATTCAAACAGGAAAACCCCGAAAAATAAAAT
CAATAATCGGCTCGCTCGACATTACAGATAATCAGGCAAAATATTTTGGCTTAAAAGCTTACAGTCAATTGAGTCCACTGATGGAAAATTGCTGTCTTTT
AATCACTGCTAACGAATCTTATCAAAGCGCCGAAAAAGATTTGGAAAAATTTACGGGGATCAAAATTTCTCACAGTACATTACAAAGATTAGTCAAGCGA
CAAGAATTTGAATTGCCTACATCTAAACAAGGAGTCAAAGAAATTACATTGGATGGCGGGAAAGTTAGACTACGCAACGAAACCAAGGGCGAGAGCTGTT
ACTGGAAAGACTATAAAGCCGTGTGTTTAGATAATGTTTATTCGGGAGCCTTTTTTCAAAATAATCAAGATTTAATTGATTGGACTAATAGCCAAAAATT
ACTACACCCTATGTATTGTCTAGGAGATGGTCATGCCGGGATTTGGAACATCTTTAAAGAAATTGGAGATAATGAACAAAGACAAGAAATTTTAGATTGG
TATCATCTCAAAGAAAATCTCTACAAGGTAGGGGGTTCAATAAAACGATTGAAATTAGCAGAAAATATGTTATGGCAAGGCAAAGTTGATGAAGTTAGAA
ATTTATTTAAAGACTTTAAAAGTCAAGCATTTAAAACGTTTTGCAATTATTTAGAGACCCATCGCTGCCGAATAGTGAATTACCAATACTACAAAGAAGA
GTCCATAAGTTCGATTGGTTCGGGAACAGTTGAATCAACAATTAAACGCATTGGATTAAGGGTGAAAATATCGGGAGCGCAATGGAATATTGAGAACGTT
TCCTCAATCCTGGCTCTTCGCTGTGCTTATCTCAACGGTCAACTTTCAATTTGATGTATGTGCCAAAGTGAGATGCACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 210 bp | 69 aa | 88 | 297 | + | No |
Description : First part of the transposase
ORF sequence :
MTPSDQQQLKAHLKAVAKILYRNTEPTELKSFESIEKSVRQKMLSEVGPEIGNFFFQQYQEFKQENPEK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 302 aa | 246 | 1154 | + | No |
Description : Second part of the transposase
ORF sequence :
LFFSAVSGIQTGKPRKIKSIIGSLDITDNQAKYFGLKAYSQLSPLMENCCLLITANESYQSAEKDLEKFTGIKISHSTLQRLVKRQEFELPTSKQGVKEI
TLDGGKVRLRNETKGESCYWKDYKAVCLDNVYSGAFFQNNQDLIDWTNSQKLLHPMYCLGDGHAGIWNIFKEIGDNEQRQEILDWYHLKENLYKVGGSIK
RLKLAENMLWQGKVDEVRNLFKDFKSQAFKTFCNYLETHRCRIVNYQYYKEESISSIGSGTVESTIKRIGLRVKISGAQWNIENVSSILALRCAYLNGQL
SI
TLDGGKVRLRNETKGESCYWKDYKAVCLDNVYSGAFFQNNQDLIDWTNSQKLLHPMYCLGDGHAGIWNIFKEIGDNEQRQEILDWYHLKENLYKVGGSIK
RLKLAENMLWQGKVDEVRNLFKDFKSQAFKTFCNYLETHRCRIVNYQYYKEESISSIGSGTVESTIKRIGLRVKISGAQWNIENVSSILALRCAYLNGQL
SI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1067 bp | 355 aa | 88 | 1154 | + | Yes |
Chemistry : DDE
ORF sequence :
MTPSDQQQLKAHLKAVAKILYRNTEPTELKSFESIEKSVRQKMLSEVGPEIGNFFFSAVSGIQTGKPRKIKSIIGSLDITDNQAKYFGLKAYSQLSPLME
NCCLLITANESYQSAEKDLEKFTGIKISHSTLQRLVKRQEFELPTSKQGVKEITLDGGKVRLRNETKGESCYWKDYKAVCLDNVYSGAFFQNNQDLIDWT
NSQKLLHPMYCLGDGHAGIWNIFKEIGDNEQRQEILDWYHLKENLYKVGGSIKRLKLAENMLWQGKVDEVRNLFKDFKSQAFKTFCNYLETHRCRIVNYQ
YYKEESISSIGSGTVESTIKRIGLRVKISGAQWNIENVSSILALRCAYLNGQLSI
NCCLLITANESYQSAEKDLEKFTGIKISHSTLQRLVKRQEFELPTSKQGVKEITLDGGKVRLRNETKGESCYWKDYKAVCLDNVYSGAFFQNNQDLIDWT
NSQKLLHPMYCLGDGHAGIWNIFKEIGDNEQRQEILDWYHLKENLYKVGGSIKRLKLAENMLWQGKVDEVRNLFKDFKSQAFKTFCNYLETHRCRIVNYQ
YYKEESISSIGSGTVESTIKRIGLRVKISGAQWNIENVSSILALRCAYLNGQLSI
Blast result :
Comments
ISOni1 is 88% aa similar to ISMiva1.
The third ORF is the putative ORFAB transposase recnstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase recnstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C., Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and Kerfeld,C. (2012) Direct submission GenBank.
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C., Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and Kerfeld,C. (2012) Direct submission GenBank.