ISNpu15

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010628 ND Nostoc punctiforme
Nostoc punctiforme PCC 73102
Nostoc punctiforme ATCC 29133
DNA section
IS Length : 1218 bp

Ends


IR Length : 20/21

IRL : GGGTGCATCCCAGTTTTTATTATTCCAAAACAAATAAATAACAGGGAAGG
IRR : GGGAGCATCCCAGTTTTTATTTAGTGAGCAAAGATTAATCCATTAAGATA

Insertion site


Left flankDirect repeatRight flankDR Length
AGACCACCGATTCACTAATCCCCTTGTCG9
TCCAAAGAGCATTAATAAGAGCCTGAGAT9
AGTTAAAGAAACGCTAGAGTTTGTCAAAT9
TAGAACTATTGTAGAGACTTAGCACTGCTA9
AAGATAAATTAGTTTATTATGATACAGCGTAAGTATATTT0
GAAATGAGCTTGTATACAAAATGAAGTCTTCAGTCGAGAG0
TGATTGCACTTTTGTCAAGATGTCATAGGCGACACTAAAA0
CTAGAACTATTGTAGAGACTAATTAATATTTGCTGAACGC0

DNA sequence

GGGTGCATCCCAGTTTTTATTATTCCAAAACAAATAAATAACAGGGAAGGATTGTTACAGAAAACGGAAGTCAGGAAGTAAGGGGATATCAGGAAGGGAT
AATAAAGTCATCAAGACTAGGTGTATCAATGACTCCTGAAGAAGAACAACAGATAAAAGAATATTCTCGTGCAATAGCAAAGATACTGTACAAAAGTACT
ACGGGTGAGCAACTCACAAGTTTGGCAAAAATAGAAGAAGTAGTACGCTCTCAAATGCGGAAGCATGTAATGCCAGAAGTAGGGTTTTTTTATCGAAAAT
GCCACAGGAGAAAGCAGAGGATACAAACGAAAAATAAAAAGTATTATTGGAGAACTGCCAATTACAAACTCTCAAGCACAAAAGCTAGAAATTAGACCCC
ATAATCAATTGAGTCCATATTTAGAAGCTTGTTGCTTACGAATCAGCGCGTCGGTATCGTATCAACGTGCGGCAGAAGATATTGAATATTTAACTGGTGT
AGAAGTATCAAAAAGTGTGCAGCAACGATTAGTGCATCGTCAAAATTTTGAATTACCGCAAGTAGAATCAACTGTGAAAGAATTGAGTGTGGATGGAGGA
AATATACGCATTCGTACAATCAAGGGACAAGTCTGTGATTGGAAGGGTTATAAAGCTACCTGCTTACATGAAAAACAAGCTATTGCTGCCTCATTTCAAG
AAAATAGTCTTGTAATTGATTGGGTCAAAAGCCAATCAATTGCTCCTATCTTGACCTGTCTTGGCGATGGCCATGACGGTATTTGGAATATTGTCCGCGA
TTTTGCTCCCGAACACCAGCGTCGGGAAGTACTTGATTGGTTTCATCTGATGGAAAACCTACACAAGATTGGCGGTTCTAATCAACGGCTCAATCAGGCT
AAAATCCTTCTCTGGCAAGGAAAAGTTGATGATGCTATCGCTGTCTTTGCTGACTGTCAGCTAAAACAAGCTTTTAATTTCTGTACTTATCTTGAGAAAC
ATCGACACCGGATTGTCAATTACCAATATTATCAAGCAGAACAAATCTGTTCCATTGGTTCTGGTACTATTGAGTCTACTGTCAAACAGATTGACCGTCG
AACCAAAATTTCTGGCGCACAATGGAAATCTGATAACGTTCCTCAAGTCTTAGCTCAACGCCAGAGCTTATCTTAATGGATTAATCTTTGCTCACTAAAT
AAAAACTGGGATGCTCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa129395+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTPEEEQQIKEYSRAIAKILYKSTTGEQLTSLAKIEEVVRSQMRKHVMPEVGFFYRKCHRRKQRIQTKNKKYYWRTANYKLSSTKARN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
884 bp297 aa2931176+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GFFIENATGESRGYKRKIKSIIGELPITNSQAQKLEIRPHNQLSPYLEACCLRISASVSYQRAAEDIEYLTGVEVSKSVQQRLVHRQNFELPQVESTVKE
LSVDGGNIRIRTIKGQVCDWKGYKATCLHEKQAIAASFQENSLVIDWVKSQSIAPILTCLGDGHDGIWNIVRDFAPEHQRREVLDWFHLMENLHKIGGSN
QRLNQAKILLWQGKVDDAIAVFADCQLKQAFNFCTYLEKHRHRIVNYQYYQAEQICSIGSGTIESTVKQIDRRTKISGAQWKSDNVPQVLAQRQSLS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1048 bp348 aa1291176+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTPEEEQQIKEYSRAIAKILYKSTTGEQLTSLAKIEEVVRSQMRKHVMPEVGFFIENATGESRGYKRKIKSIIGELPITNSQAQKLEIRPHNQLSPYLEA
CCLRISASVSYQRAAEDIEYLTGVEVSKSVQQRLVHRQNFELPQVESTVKELSVDGGNIRIRTIKGQVCDWKGYKATCLHEKQAIAASFQENSLVIDWVK
SQSIAPILTCLGDGHDGIWNIVRDFAPEHQRREVLDWFHLMENLHKIGGSNQRLNQAKILLWQGKVDDAIAVFADCQLKQAFNFCTYLEKHRHRIVNYQY
YQAEQICSIGSGTIESTVKQIDRRTKISGAQWKSDNVPQVLAQRQSLS

 

Blast result :
Comments
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISNpu15 is 70% aa similar to ISMae42.
References