ISMae18

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010296 ND Microcystis aeruginosa
Microcystis aeruginosa PCC 7806
Microcystis aeruginosa NIES-843
DNA section
IS Length : 1179 bp

Ends


IR Length : 32/38

IRL : GGGAGCATCCCAATTTTGCCATAAGTATAAATGCTTAGAGACTAAAAACG
IRR : GGGAGCATCCCAGTTTTGCAATGAGTAAAAATACTCAGGCAATATCGCCG

Insertion site


Left flankDirect repeatRight flankDR Length
TGTTAAGGATATTTCCGGTTGACCTGTCC9
CAAAAAGTCTCTCAAAACACCAAACAAAAC9
ACAGGGAATTCTAGCATCTGC0
TTACTCGCCATTGGTGCTTTTGGGTGCGGGT9
AAAACTGATAGAATAGAACTA0
ATATGTTCTATATTCTCGCCAATATTCTA9

DNA sequence

GGGAGCATCCCAATTTTGCCATAAGTATAAATGCTTAGAGACTAAAAACGTTAGCTAAGGCAAAATAAAGAGAAACAATTAAACGGTAGAATCATGAATA
CAGACCAAAAAGAACAACTAGATCAACATTTAAAAGCCATTGCTCAAATTCTAGTCGATAATACCCCAGAAGAACAACTACGTAGCTTTGAGGGCATTGA
AACCGCCCTGCGAGACCATTGGCTGACTACATTGGGTCCTGCCATAGGCAATTTTTTTTTGAATCAGCAACAGGAACCCAAGCAGGGCGAACCAAAAGCG
TAAGCAGTATCATAGGGAAAGTCAAGATAAGCGAGAAACAAGCCGATAAACTAGGATTAAGCAAGAATAATCGATTAAGTCCCATGCTGGAGAAATGTTG
CTTAGGAGCAGTGGCTAAAGTCTCTTTTGAAGATGCGGAAAAAGCTATCAAAATGGCGACAGGAATGGCAGTTTCAGGCAGTAGTCAACAGAGGCTTGTA
CAAAGATATAAATTTGAGGAAGCAGAAGCAAAGAGTCCGGTAGAAGCATTGAGTGTAGAGGTCGGAAAAGTCAGAATCAGAACGCCCAAAGGACAACCGA
GTCAAGGTCGAGATTACAAAGCAGTAAGTCTGCATGGTCAAGAATGTGCGGGATTTTTTCAGCAAAATGAAGAATTACTGGAATGGGTGAACCGTCAGCC
CTTAACAGAGGTAGTCACCTGTTTAGGAGATGGTCATGATGGGGTGTGGAATCTGATGGAGAAAATCGGTGTTAAAAGGAGAGAAATATTGGATTGGTAT
CATTTAGTAGAGAATATGAAGAAAATAGGCGGGTCAAACAAGCGTCTGAATAGAATCAAAGAGAATTTATGGAAAGGAGAGGTGAAAAGAGTTTTAGAGG
AATTAGAGGGATGCAAAAAGAAACAGGCTATAAATTTCACCAAATATGTCGATAAACATCGAGAAAGAATACCCAATTACGAACTCTATCAATCACAAGG
GATTTGCATCGGTTCTGGAAGTGTAGAGTCAAAGATTAAGCAAATAGGTGCAAGAATGAAAATTGTGGGAGCACAATGGAAAGCAGAGAATGTTCCTCAG
TATTTGAAGCTACGTTGTGCTTATCTCAACGGCGATATTGCCTGAGTATTTTTACTCATTGCAAAACTGGGATGCTCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
210 bp69 aa94303+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MNTDQKEQLDQHLKAIAQILVDNTPEEQLRSFEGIETALRDHWLTTLGPAIGNFFLNQQQEPKQGEPKA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
945 bp314 aa2011145+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NRPARPLADYIGSCHRQFFFESATGTQAGRTKSVSSIIGKVKISEKQADKLGLSKNNRLSPMLEKCCLGAVAKVSFEDAEKAIKMATGMAVSGSSQQRLV
QRYKFEEAEAKSPVEALSVEVGKVRIRTPKGQPSQGRDYKAVSLHGQECAGFFQQNEELLEWVNRQPLTEVVTCLGDGHDGVWNLMEKIGVKRREILDWY
HLVENMKKIGGSNKRLNRIKENLWKGEVKRVLEELEGCKKKQAINFTKYVDKHRERIPNYELYQSQGICIGSGSVESKIKQIGARMKIVGAQWKAENVPQ
YLKLRCAYLNGDIA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1052 bp350 aa941145+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNTDQKEQLDQHLKAIAQILVDNTPEEQLRSFEGIETALRDHWLTTLGPAIGNFFFESATGTQAGRTKSVSSIIGKVKISEKQADKLGLSKNNRLSPMLE
KCCLGAVAKVSFEDAEKAIKMATGMAVSGSSQQRLVQRYKFEEAEAKSPVEALSVEVGKVRIRTPKGQPSQGRDYKAVSLHGQECAGFFQQNEELLEWVN
RQPLTEVVTCLGDGHDGVWNLMEKIGVKRREILDWYHLVENMKKIGGSNKRLNRIKENLWKGEVKRVLEELEGCKKKQAINFTKYVDKHRERIPNYELYQ
SQGICIGSGSVESKIKQIGARMKIVGAQWKAENVPQYLKLRCAYLNGDIA

 

Blast result :
Comments
ISMae18 is 69% aa similar to ISMae42.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
References