ISAlac1

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_017167 ND Alicyclobacillus acidocaldarius
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1
DNA section
IS Length : 1608 bp

Ends


IR Length : 17/24

IRL : GAGCGTATAAAGTTTGAGTAGGGGTGTTCTCGGGAGGATTTTCCGGGGAC
IRR : GTCCGTGTCAAGTTGTCGTAGGGGAGGACCTCACCGCTTAGGTTGAGTCA

Insertion site


Left flankDirect repeatRight flankDR Length
TGGCCGTTTGTGGGAATTGTTGAGGATTG9
ACGGCTCGCATGGCGGGTTACCGCGGTTG9
GAGTCGACGTGTAGCGTACGCGTGCCTTG9
AGTCGAACCGTGTCCGAAAGGCTGGCGTGG10
GATGTCCCAAATGAGAAATTTTGTGCTTG9
CGTGGCGCTCGCAGCCGCGCTCCGGCTTC9

DNA sequence

GAGCGTATAAAGTTTGAGTAGGGGTGTTCTCGGGAGGATTTTCCGGGGACAAAGAGAAGAGACCTCACTGAGCACAAAAACAACCCGCGTTCGGAGCGCG
GATGAAGAGGTGAGGTCTCTCATGTTGGATATTCGTGATGGCGCGGCGGGTTTCCTGCCGCTGTGGATGAATCTGTTGTGGTTGTCTGAGCATCCGTGCG
ACTTCGCGATTCTTGAAGAGAGGCTCATGAGGTCCTTCGGCGAATTCATGAGGGAAATGGTTCCGCGCTTGCTCGAAGCGTGGGACGAGAAGCTGGCGGC
GGAGCGTGACCGAAATGAGTGGGAACTTATTCAGCGTAAACCACGGACGGTCGTGTCCACCCTGGGAGAGATCACGTATCGTCGGCGTTACTACCGCAAT
CGAAAGACGGGCGAACGTCGCTTCCTGTTGGATGAGGTAGCAGGGTTCGAACCCCGTCGTCGATTGAGCGGGAAGTTAAGAGACCAAGCTGTGGCGTTGG
CTTTAGATGTGTCGTACCGGCGCGCGGCAGAGATTCTACAGACTTGGGTGCCGGACATCAGCGCGATGGCGATTTGGCAAGAAGTGCAAAGGCTCGGAGG
GGAGAAGCGCGTCCGAGCGGAGCTGGAGCGGGAGAAGGTGTTCGAACAAGGAGACGCTCCGGGAGGGAGACGCAAAGCCGAGACACTCTTCGTGGAAGCG
GATGGCGTGTATGTCCGAGGGCGCAGGGAGGAAGATGGCCAGTCACACTTCGAATTGAAGGTGGCCGTGGCGTATGAAGGCAAGCAGGAACAAGGGCGGG
AACGGCGCGCCCTGATGAATCGACAGGTGGTCGCGGGGACTGAGGAAGCTGAACGCTTCTGGGAGGAAGCGGTGTCCTCTTTTGGGCGCGTGTGGGATTG
GGCCAGCGTGAAACGATGTTGGCTCGGCGCGGACGGGGCGGAATGGGCGAAGAAGGGCGTCGGAATGCTCCCTGGGGCTCGATATCGATTGGATCCCTTT
CACCTGCGGCAGGCGATGGTTGCGGCCTTGGGACGGGAGACGCAGGCCTACCGGAAGGTATGGGATGCGGTGAGAACCGAGGAGTGGTCGCGGGTAGAAA
AGGTGCTGACGAAGATGGAGAAGCAAAGCGAAGGGGCGAAGAAGCAGCGTATCTCGAAGCTGAAATCATACATGGAGAAGAATTGGCAAGGCATTGTGGG
CGATGATGATGTGGTGAGTCTGGGTGCAATTGAGGGCCAGGTGTTCCACCACGTGGCGCGACGGATGAAGCGCCATGGGGCACGTTGGAGCAAGCCAGGG
GCGGATGCGTTAGTGCGGCTGATTGCAACGCGCGCCAATCACGAGCCTCTGCGGGCCAACACGCCTTCTCGTTCGGAAATGCAGGCGGAAGTGCCGGTGA
AGACAAAGCCAGTGAACGAGGCGGAAATCGCGAGACGGGTCGAAGAAGCGGCGACTTGGCTGGAAAAACACATGCCAGCATTGGTCGGTCCGCATGCGGA
CCGACCATGGGTGAAGTACGTGCTTCGTGAGCTCGCCAGAGTTTCAGCCACGGTTGCCTGACTCAACCTAAGCGGTGAGGTCCTCCCCTACGACAACTTG
ACACGGAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1437 bp479 aa1221558+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLDIRDGAAGFLPLWMNLLWLSEHPCDFAILEERLMRSFGEFMREMVPRLLEAWDEKLAAERDRNEWELIQRKPRTVVSTLGEITYRRRYYRNRKTGERR
FLLDEVAGFEPRRRLSGKLRDQAVALALDVSYRRAAEILQTWVPDISAMAIWQEVQRLGGEKRVRAELEREKVFEQGDAPGGRRKAETLFVEADGVYVRG
RREEDGQSHFELKVAVAYEGKQEQGRERRALMNRQVVAGTEEAERFWEEAVSSFGRVWDWASVKRCWLGADGAEWAKKGVGMLPGARYRLDPFHLRQAMV
AALGRETQAYRKVWDAVRTEEWSRVEKVLTKMEKQSEGAKKQRISKLKSYMEKNWQGIVGDDDVVSLGAIEGQVFHHVARRMKRHGARWSKPGADALVRL
IATRANHEPLRANTPSRSEMQAEVPVKTKPVNEAEIARRVEEAATWLEKHMPALVGPHADRPWVKYVLRELARVSATVA

 

Blast result :
Comments
ISAlac1 is 58% aa similar to ISTph1.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References