ISAmo2

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_018024 ND Anaerobaculum mobile
Anaerobaculum mobile DSM 13181
DNA section
IS Length : 1566 bp

Ends


IR Length : 23/28

IRL : GATCGCGTAAAATATTTGTAGGAGTTTTTTAGGTAAAAAATAAAGGGACC
IRR : GACCGCGTCAAGTTTTTGTAGGATTTTTAGGAACCACTCCCATGTATCTT

Insertion site


Left flankDirect repeatRight flankDR Length
CAACTTATTGCTTTAACGTCGTTTAATT8
CGCCGAGTAGGTAAACTTAAGACCCCTTG9

DNA sequence

GATCGCGTAAAATATTTGTAGGAGTTTTTTAGGTAAAAAATAAAGGGACCGCTCCTTTTGGTAGAATCTTTGTGTGACCAAAAACTCATAAAGGAGGGTC
CCAACTTGAACAAAGTTATTTTACTACAAATTGTCTCAAACTTCATCTCCGAGATATTGAAGTTTTTTTGCAGTTCCAATGTCAGGACGCTGGCTGAAAT
AGAAGATGAGCTTTTTCGCATGACGAAGGCCTTCATCCGGGAGATCGTAAAGGCTTACCTTGAGCTTGCAGACGAAGCCATATTAAAGGACAAAACGTCT
CGAAAGCAAAGAGGATTGGTCGTAGAAAGGCGTGAGGACAGAAGGAGCTTATATACCATCTTTGGTGATATATCTTTCAAGAGGACCTACTACTTCGACA
AAAGTCATAAAAAATATGTATACCCCCTGGACGAAGCATTAAGGTTGGATAAACGTCAAAGGTTGAGCAAAACAGTAACGGTAAAGCTGGTAGAGACGGC
AGGCCAGGTATCTTACGCCAAAAGTTCGATGAACGTGACTGAGGGTGAGTTATCCAAGCAGACGGTAAAAAACAAGATCCACAGCCTAAAGCTGGAGGCC
TTAAAGACGAAAGTTCCTAAAAAAAGAGGCGTAGAGGTATTGCATATAGATGCAGATGAAGACCACGTTTCCCTACAGGAGGGCAGAAATGTGAATCTTC
CTTTAGTCTGCATATACGAGGGCACCTTTAAAGAGGGGTCCAAAAACAGATGTATAAACCCCATATACATGAGCGGTTACGGAAAGGACACAGATGAGTT
TTGGCTTGAGGTAGCAGATAGGATATACGAGCTTTACGACCCGGAGGACATAAAGGACATATACATCCATGGAGATGGTGCAAATTGGATAAGGCAAGGT
ATAAGCTGGCTGCCCGAGTCAAAGCTGGTGCTGGATAAATTTCACCTAAATAAAGCGATCCTTGAATCCACGGCAAGGCAGCCTGAAAAAAGACACGAAA
TATACAAAGCCATAAGGGAAAACGACATGAATTCCTTTAAAAGGATCATTCTAGATATGTTAAATGATGCCTCTGATGAAAAGGAAAGAAAAAGGATAGT
AGACTTCAGGAGGTATGTGACCAACAACTGGAACTCCATTACCATTCGAAACGAAGAGGACTGCGGCAGCTCAAGCTCCGAGGGTCACATTAGTCACGTG
CTTTCCTCTAGGTTAAGTTCCAGGCCTATGGCTTGGAGCAGGAAGGGCTTAAAGGCCATGTCTGCTTTAAGGGCTTACATCTGTAGTGGTGGGAAAGTGA
AAGCGGAACACGTAGGGGGAAACGACCAAAAGGGCGACAAGACAGACAAAGGACACAACTTCACATTAAACTTAGAAGATATCTTTAGTTCGGCTGCAAG
CGAGATAGGCTCCATAACAGTACTTAACATGGGGAAGGTAACGCCTTTATACACGACCTTAAAGGGCATATGTCATGGCGGGTTTGATTTTAGCTGAACT
AAAGACATTGCCCCTTAAGATACATGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1392 bp463 aa1061497+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNKVILLQIVSNFISEILKFFCSSNVRTLAEIEDELFRMTKAFIREIVKAYLELADEAILKDKTSRKQRGLVVERREDRRSLYTIFGDISFKRTYYFDKS
HKKYVYPLDEALRLDKRQRLSKTVTVKLVETAGQVSYAKSSMNVTEGELSKQTVKNKIHSLKLEALKTKVPKKRGVEVLHIDADEDHVSLQEGRNVNLPL
VCIYEGTFKEGSKNRCINPIYMSGYGKDTDEFWLEVADRIYELYDPEDIKDIYIHGDGANWIRQGISWLPESKLVLDKFHLNKAILESTARQPEKRHEIY
KAIRENDMNSFKRIILDMLNDASDEKERKRIVDFRRYVTNNWNSITIRNEEDCGSSSSEGHISHVLSSRLSSRPMAWSRKGLKAMSALRAYICSGGKVKA
EHVGGNDQKGDKTDKGHNFTLNLEDIFSSAASEIGSITVLNMGKVTPLYTTLKGICHGGFDFS

 

Blast result :
Comments
ISAmo2 is 91% aa similar to ISAmo1.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References