ISAmde4

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013385 ND Ammonifex degensii
Ammonifex degensii KC4
DNA section
IS Length : 1584 bp

Ends


IR Length : 21/25

IRL : GTTAGTGTAAAGTTACTGTAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAA
IRR : GATCGTGTCAACCTACTGTAGGAGTCTCGATCCCCACCGTCCCTGAGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
GCAAGCGAAGCGCGGGAAAA0

DNA sequence

GTTAGTGTAAAGTTACTGTAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAACGAAGAGAGACCTCACCGTCCTTCGAAGGGACGAGCAGTTGACAATCCAA
CCACTCAGGAGGGTGAGGTCTCTATGCAGATTATAAAGCAGATTTGGGAGAAGTTCCAGAGGGTAGCGGAGCTAACGTTAAAGATTCTGGAGGAAGGGAT
CGACCTTTTAAGCTTCGAGGAGAAGCTCTGGCAGGAGCTTAAAAGCTTAGGAAAAGAGATCCTGAGGGAAGTTTTGGAGGCAAAAGACGCTTATTTACGG
GAGCACCGGGAAAAGCGGCCTGGCTGGCAAGTAGAGCGGAGGAATGATCCCAAGGAAGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTATT
ACCGGCACAAAGAGACTGGGGAGCGGGCATACTTAATAGACCGTTTGGTGGGTTACGGGCCGCATACGCGGGTTGACCCTCTGGTCAAGGCACAAGTTTT
AGAAGAAGCAGTAGAGCTTTCTTACCGGAAAAGTGGGGAAAGAGTAGGGAAAGGTAGCCAGGAAGTTGTACTGAGTGGCGAGGCTGTGGAGAAGGTAGTG
CACGACTTTATTCCTGAAGAGCTGCCCGAACCACCCAACGATAAGCGCCGGGTAAAGGTTCTTTATGTGGAGGCGGATGAAGACCACGTAGCAGGTCAGG
ACGGGAAGAAGTACTTACCCTGGCTGGTTTACATCCACGAGGGGAAAGAGAAGGTGGGGAAAGACAGATACAGACTCAAGCGGCCTTATTACTTGGGAGG
GCTTTACTCAGATACGGATGAGCTGTGGTTCGAGGTGTTGAGCTACATCGAGGGGCAGTACGAATTATCTCCTGTGGAGCGGACTTATCTCTGCGGTGAC
GGAGACAGGTGGATAAAGAAAGGGCTGGAGTTTCTACCGAGGAGCGTTTTTGTCTTAGATCTTTTTCACCTGGACAAGTACCTGGTGGCTGCTTTGGGGC
GTGATAGTGATGGTTATAGAGAGATTTGGGCGGCCTTATGGGATGGTGATGAGGTAAAGGTACAGGCGATTTTGCGGCGGGCAGGCAAAGCTGCGGCGAG
TCCTGCACGGAAGGGAGCAGTACGGGATTGCCGATGTTACATCCGGCAGAACTGGGAGGGGATAACGGCATACCGGCTTTACCCGGAGGCGCAGTTAGGG
GTGAGCGCGGAGGCGCACGTAAGCCACCTGTACTCGGCGCGGTTGTCGTCGCGGCCGATGGCCTGGAGTGCTTGTGGGGTAGACCGGATGACCCGGTTGC
GGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAGGA
AGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCATTACGGGGTCCGGTAACATCATTAACCAGAGCCCTCAAAGCTTTG
AGCCGGAACATCGATCTTCTCTGGTAGTTCGTCCTGGCTCAGGGACGGTGGGGATCGAGACTCCTACAGTAGGTTGACACGATC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1404 bp467 aa1241527+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQIIKQIWEKFQRVAELTLKILEEGIDLLSFEEKLWQELKSLGKEILREVLEAKDAYLREHREKRPGWQVERRNDPKEVLTLFGLVSYKRTYYRHKETGE
RAYLIDRLVGYGPHTRVDPLVKAQVLEEAVELSYRKSGERVGKGSQEVVLSGEAVEKVVHDFIPEELPEPPNDKRRVKVLYVEADEDHVAGQDGKKYLPW
LVYIHEGKEKVGKDRYRLKRPYYLGGLYSDTDELWFEVLSYIEGQYELSPVERTYLCGDGDRWIKKGLEFLPRSVFVLDLFHLDKYLVAALGRDSDGYRE
IWAALWDGDEVKVQAILRRAGKAAASPARKGAVRDCRCYIRQNWEGITAYRLYPEAQLGVSAEAHVSHLYSARLSSRPMAWSACGVDRMTRLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLLW

 

Blast result :
Comments
ISAmde4 is 94% aa similar to ISAmde3.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References