ISAmde3

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013385 ND Ammonifex degensii
Ammonifex degensii KC4
DNA section
IS Length : 1586 bp

Ends


IR Length : 18/23

IRL : GTTAGTGTAAAGTTACTGTAGGAATTTTGCAGGAGCTAAGGAAGGGGAGA
IRR : GATCGTGTCAACCTACTGTAGGAGTCTCGAGCCCCACCGTCCCCGGGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CTTCTTGACCTAGAGCCTCCGCTTGCTTG9
ACCTTTTTCCCACAGGCGGC0

DNA sequence

GTTAGTGTAAAGTTACTGTAGGAATTTTGCAGGAGCTAAGGAAGGGGAGAAAGAAGAGAGACCTCACCGTCCTTGCAACGGACGAGTAGCTGCTCAAACA
ACTACTCAAGAGAGGTGAGGTCTCTATGCAGATTGTAAAACAGATTTGGGAAAAGTTCCAGAGGGTAGCGGAGTTAACGTTTGAGGTATTAGAGAACGGG
ATTGATTTTATAAGCTTTCAGGAGAAGCTCCGGCGGGAACTCGACAGCTTAGGGCAAGAGATCCTGAGGGAAGTTTTAGAGGCGAAAGACGCTTATTTAC
GGGAACATCGGGAAGAGCGGCCTGGCTGGCGAGTAGAGCGGCGAAACGACACTAAAGGGGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTA
TTACCGGCATAAAGAGACTGGGGAGCGGGCGTACTTAATAGACCGGTTGGTAGGTTACGGGCCGCACGCGCGGGTTGATCCTCTGGTTAAAGCACAAGCC
TTAGAAGAAGCGGTAGAGCTTTCCTATCGGAAGAGTGGCGAGAGGGTAGGGAGAGGCAATCGGGAGGTTGTGCTAAGTGGCGAAGTGGTGAAGAGGGTGA
TTCACAACTTTACACCGGAAGAACTGCCCGAACCACCCAAAGAGAAGCGCCGGGTAAAGGTTCTTTATGTAGAGGCGGATGAAGACCACGTAGCAGGTCA
GGACAGAAAGAAGTACTTACCCCGGCTGGTATACATCCACGAGGGGAAAGAGAAGGCGAGGAAAGACAGATACAGACTCAAGCGGCCTTATTACCTGGGA
GGGCTTTACTCAGATACGGATGAGCTGTGGTTCGAGGTGTTGAGCTACATCGAGGAGCAGTACGAATTATCTTCTGTGGAGCAGATTTATCTCTGCGGTG
ACGGAGACAGGTGGATAAAGAAAGGGCTGGAGTTTCTACCGAGGAGCGTTTTTGTCTTAGATCTTTTTCACCTGGACAAGTACCTGGTGGCTGCTTTGGG
GCGTGATAGTGATGGTTATAGAGAGATTTGGGCGGCCTTATGGGAGGGTGATGAGGTAAAGGTACAGGCGATTTTGCGGCGGGCAGGCAAAGCTGCGGCG
AGTCCTGCACGGAAGGAAGCGGTACGGGATTGCCGACGTTACATCCGGCAGAACTGGGAGGGGATAATGGCCTACCAGCTTTATCCCGGGGCGGAGTTGG
GGGTAAGTGCGGAGGCGCACGTAAGTCATCTCTTCTCGGCACGGTTATCATCCCGACCGATGGCTTGGAGTGCTTGTGGGGTAGACCGGATGGCCCGGTT
GCGGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAG
GAAGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCTCTACGGGGTCCGGTAACATCATTAACCAGAGCCCTCAAAGCTT
TGAGCCGGAACATCGATCTTCTTTGGTAGTTCGTCCTGGCCCGGGGACGGTGGGGCTCGAGACTCCTACAGTAGGTTGACACGATC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1404 bp467 aa1261529+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQIVKQIWEKFQRVAELTFEVLENGIDFISFQEKLRRELDSLGQEILREVLEAKDAYLREHREERPGWRVERRNDTKGVLTLFGLVSYKRTYYRHKETGE
RAYLIDRLVGYGPHARVDPLVKAQALEEAVELSYRKSGERVGRGNREVVLSGEVVKRVIHNFTPEELPEPPKEKRRVKVLYVEADEDHVAGQDRKKYLPR
LVYIHEGKEKARKDRYRLKRPYYLGGLYSDTDELWFEVLSYIEEQYELSSVEQIYLCGDGDRWIKKGLEFLPRSVFVLDLFHLDKYLVAALGRDSDGYRE
IWAALWEGDEVKVQAILRRAGKAAASPARKEAVRDCRRYIRQNWEGIMAYQLYPGAELGVSAEAHVSHLFSARLSSRPMAWSACGVDRMARLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLLW

 

Blast result :
Comments
ISAmde3 is 91% aa similar to ISAmde2.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References