ISAmo1

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_018024 ND Anaerobaculum mobile
Anaerobaculum mobile DSM 13181
DNA section
IS Length : 1572 bp

Ends


IR Length : 22/28

IRL : GATAGCGTAAAATATTTGTAGGAGTTTTTTAGGGAGGAAAAATAAAGGGG
IRR : GACCGCGTCAAGTTTTTGTAGGATTTTTAGGAACCACTCCCCGATGTATC

Insertion site


Left flankDirect repeatRight flankDR Length
GCCAGGGATACTTACAGGTTGGTCCAGG8
TTGGCGCGGTCGATAGATCAATTTGCCTT9
GTGTGTCTATCAAATCCTAC0

DNA sequence

GATAGCGTAAAATATTTGTAGGAGTTTTTTAGGGAGGAAAAATAAAGGGGCCGCTCCTTTTGGTAGAATCTTTGTAGCACACAAAAATTCATAAAGGAGG
GTCCCAACTTGAACAAGGTTATTTTACTACAAATTGTCTCAAACTTCATCTCCGAGATATTGAAGTTTTTTTGCAGTTCCCATGTCAGGACGCTGGCTGA
AATAGAAGATGAGCTTTTTCGCATGACGAAGGCCTTCATCCGGGAGATCGTAAAGGCTTACCTTGAGCTTGCAGACGAAGCCATATTAAAGGACAAAACG
TCTCGAAAGCAAAGAGGATTGGTCGTAGAAAGGCGTGATGACAAAAGAAGCGTTTATACCATATTTGGAGATATATCCTTTGACAGGACCTACTACTTTG
ATAAAAGTCATGATAAATACGTATACCCCTTAGACGAAGCCCTGGGGTTGGATAAATACGAAAGGATAAGCAAAACAGTGACGGTAAAGCTGGTAGAGAC
GGCAGGCCAGGTATCTTACGCTAAAAGTTCTTCAAACGTGACTAGTGGTGAGTTATCCAAACAAACCGTCAAAAACAAGATACACAGCCTAAACCTGGAG
GCCTTAAAGACAAAGATCCCCGAGAAAAGAAGCGCGCATGTATTGCACATAGATGCAGATGAAGACCACGTATCCTTACAGGAGGGCAGAAATGTGAATC
TTCCTTTGATTTGCATATACGAGGGCACCTTTAAAGACGGATCTAAAAATAGATGTATAAACCCCATATACATGAGCGGTTACGGAAAAGACGCAGACGA
GTTTTGGCTTGAGGTAACAGATAGGATATACGATCTTTACGACCCGGAGGACATAAAGGACATATACATCCATGGAGATGGTGCAAATTGGATAAGACAG
GGGATAAACTGGCTGCCCGAGTCAAAGTTGGTGCTCGATAAATTCCACCTAAATAAAGCGATCCTTGAATCCACGGCAAGGCAGCCTGAAAAAAGACGTT
ATATATACAGGGCCATAAATACAAATGACCTTAACTCCTTTAAAAAGATTAGCTTTGAGATGTTAAACGATGCCCTGGATGAAAAGGAAAGAAGGAGGAT
AAAAGATTTCAGGAGGTATATAACCAACAACTGGCAGTCTATAACGATTCGAAACGAAGAGGACTGTGGCAGCTCAAGCCCTGAAGGTCACGTAAGCCAC
GTACTTTCTTCTAGGTTAAGTTCCAGGCCTATGGCCTGGAGCAGGAAGGGCTTAAAGGCCATGTCTGCTTTAAGGGCTTACATCTGTAGCGGTGGCATGG
TGACGTCAGAACAGGTAAAGAAAAAAGACCAAGAAGGTGAGAATGCGGACAAAAGACACAAATTCACCTTGAACCTGGGGGATATCTTCGGTTCCGTTGC
AAGTGAGCTTGGATGCATAACGGTACTTAAAACGGGAAAAGTAACGCCTTTATACACTTCTTTAAAGGGCATATGTCATAGCGGGTTTGATTTTTAGCTG
ACTCGCAGACGTTACCCTTTAAGATACATCGGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1389 bp462 aa1091497+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNKVILLQIVSNFISEILKFFCSSHVRTLAEIEDELFRMTKAFIREIVKAYLELADEAILKDKTSRKQRGLVVERRDDKRSVYTIFGDISFDRTYYFDKS
HDKYVYPLDEALGLDKYERISKTVTVKLVETAGQVSYAKSSSNVTSGELSKQTVKNKIHSLNLEALKTKIPEKRSAHVLHIDADEDHVSLQEGRNVNLPL
ICIYEGTFKDGSKNRCINPIYMSGYGKDADEFWLEVTDRIYDLYDPEDIKDIYIHGDGANWIRQGINWLPESKLVLDKFHLNKAILESTARQPEKRRYIY
RAINTNDLNSFKKISFEMLNDALDEKERRRIKDFRRYITNNWQSITIRNEEDCGSSSPEGHVSHVLSSRLSSRPMAWSRKGLKAMSALRAYICSGGMVTS
EQVKKKDQEGENADKRHKFTLNLGDIFGSVASELGCITVLKTGKVTPLYTSLKGICHSGFDF

 

Blast result :
Comments
ISAmo1 is 91% aa similar to ISAnhy1.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References