ISEnfa5
- Family IS3
- Group IS150
Isoform Synonym(s) ISEnfa6
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002208 | ND | Enterococcus faecalis | Streptococcus suis S10 plasmid pStrcfr Enterococcus faecalis EF-01 plasmid pEF-01 |
DNA section
IS Length : 1686 bp
Ends
IR Length : 26/35
IRL : TGATATGCTCCCCAATAGGTAGACAGACGAAATAACAAATCTGTTTATCT
IRR : TGAACTGCTCCCCGTCAAGTAGACAGGGTAAATAATGAAAACCAGCCTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCCAATAAT | CATTATGTTG | 0 | |
| CAGAGACATA | GAT | ATCGATTTTG | 3 |
DNA sequence
TGATATGCTCCCCAATAGGTAGACAGACGAAATAACAAATCTGTTTATCTGATTGGGGAGTGTTTTTTTATGGCGAGAAGTCGGCATACGATCGAACAAA
AATTGAGTGCGCTGCGCATGATGGAAGAAGAAACATATACTTGGAAAGAAATCATGGAGGCTCATGGCGTCTCTAGGCACACCCTCCAGGTGTGGAAAGT
GAAATTCGATACCGGTGGGATCGATGCGTTGAAGGAATCGAAGGCATGGAAACTGTACACCAAGGAACTGAAAATCGCGGCCGTCCGCGACTATCTCGAT
GGGTTCACTAGAATGGAGGTACTCTCCAAGTATCAGATCAGTGATTGGTCTGTTCTTCGTAAATGGATTAAGAAGTATACTAGTCATAGCGAGTTAACAG
ATTCGAGAAAAGGGATGGATCGAGCTATGACAAAAGGAAGAAAAACTACATTCGAAGAGCGCCTTGAGATCGTGAAATATTGTTTGGAGAATGGACGCAA
CTACCAGCAGACCGCGGAACAATTTCAGGTGTCCTATGCCCAGGTCTATGGTTGGATCAAAAAGTATGACACGAACGGGGCAGAAGCGCTCGTGGACCGC
CGTGGTCGTACGAAGCCAGAGGACGAACTAAGCGAAGAAGAGAAGCTGAAACGCCGGATCCAGCAAATGGATCTTGAGAACGAACTTCTTCGTGCGGACA
TCTTGTTTCTAAAAAAGTTAGAGGAGATCGAGAGGAGGTCATGATCAGCCAGGTACGGCTACAGCAACGATATATCGCCATCAAAGAATTGAACGAAGAG
GAGGCCCTTTCGATCGTGCTCCTCTGTCGAGTCGCCGGCATCTCACGGGCGGCCTATTACAAATGGTTGAATCGCACCATACCGGTGCGTCAAGAAGAGA
ACGAGAAGCTTCTGGAGGACATCCGTCTACTCTATGACCAGGCGAACGGCACATACGGGTACCGTCGGATCACGATGACGATCAACCGGCTGCGCCGGCA
ACAAGGCCTGTCCCCGTTCAATGAAAAGCGAGTCTATCGCTTGATGCATATCCATGGGATCCAATCGGTCATCCGCCGGAAGCGGAAGAAGCATAAGAAG
TCAACGCCGCAGCACGTGGCCGAGAACCTGATGAACCGAGAGTTCAGCGCGTCCCGACCGGACGAGAAATGGTGCACCGACGTCACCGAGTTCAAATATG
GTGCCGGGAAGAAGGCATACCTGAGCGCGATCATCGACCTGTATGACGGCTCGATCGTCGCCTATCGCATCGGGAAGTCCAACAACAACGCTCTCGTCTT
CCAGACGATGATGCCGGCCATCGCGGGGCTCCGTACAGGAGCACGTCCGATGATCCATAGCGACCGAGGGTTCCAATATACCTCGAGAGGGTTCAAACGA
ATGGTAGAAGACGCGGGAATGACCCGCAGCATGTCCCGGGTCGGACGTTGCATCGACAACGCCCCGATCGAGAGTTTTTGGGGCACCTTGAAAGTGGAGA
TGTACTACTTGCGTGAGTTCCAGGCCTATAGCGAACTCACAGCGGCCATCGAGAACTACATATCGTTCTACAACCACGATCGTCTCCAGAAACGACTGAA
CGGCTTGAGCCCTGTCGAATACAGGTCTCAAGCCGCTTAGGCTGGTTTTCATTATTTACCCTGTCTACTTGACGGGGAGCAGTTCA
AATTGAGTGCGCTGCGCATGATGGAAGAAGAAACATATACTTGGAAAGAAATCATGGAGGCTCATGGCGTCTCTAGGCACACCCTCCAGGTGTGGAAAGT
GAAATTCGATACCGGTGGGATCGATGCGTTGAAGGAATCGAAGGCATGGAAACTGTACACCAAGGAACTGAAAATCGCGGCCGTCCGCGACTATCTCGAT
GGGTTCACTAGAATGGAGGTACTCTCCAAGTATCAGATCAGTGATTGGTCTGTTCTTCGTAAATGGATTAAGAAGTATACTAGTCATAGCGAGTTAACAG
ATTCGAGAAAAGGGATGGATCGAGCTATGACAAAAGGAAGAAAAACTACATTCGAAGAGCGCCTTGAGATCGTGAAATATTGTTTGGAGAATGGACGCAA
CTACCAGCAGACCGCGGAACAATTTCAGGTGTCCTATGCCCAGGTCTATGGTTGGATCAAAAAGTATGACACGAACGGGGCAGAAGCGCTCGTGGACCGC
CGTGGTCGTACGAAGCCAGAGGACGAACTAAGCGAAGAAGAGAAGCTGAAACGCCGGATCCAGCAAATGGATCTTGAGAACGAACTTCTTCGTGCGGACA
TCTTGTTTCTAAAAAAGTTAGAGGAGATCGAGAGGAGGTCATGATCAGCCAGGTACGGCTACAGCAACGATATATCGCCATCAAAGAATTGAACGAAGAG
GAGGCCCTTTCGATCGTGCTCCTCTGTCGAGTCGCCGGCATCTCACGGGCGGCCTATTACAAATGGTTGAATCGCACCATACCGGTGCGTCAAGAAGAGA
ACGAGAAGCTTCTGGAGGACATCCGTCTACTCTATGACCAGGCGAACGGCACATACGGGTACCGTCGGATCACGATGACGATCAACCGGCTGCGCCGGCA
ACAAGGCCTGTCCCCGTTCAATGAAAAGCGAGTCTATCGCTTGATGCATATCCATGGGATCCAATCGGTCATCCGCCGGAAGCGGAAGAAGCATAAGAAG
TCAACGCCGCAGCACGTGGCCGAGAACCTGATGAACCGAGAGTTCAGCGCGTCCCGACCGGACGAGAAATGGTGCACCGACGTCACCGAGTTCAAATATG
GTGCCGGGAAGAAGGCATACCTGAGCGCGATCATCGACCTGTATGACGGCTCGATCGTCGCCTATCGCATCGGGAAGTCCAACAACAACGCTCTCGTCTT
CCAGACGATGATGCCGGCCATCGCGGGGCTCCGTACAGGAGCACGTCCGATGATCCATAGCGACCGAGGGTTCCAATATACCTCGAGAGGGTTCAAACGA
ATGGTAGAAGACGCGGGAATGACCCGCAGCATGTCCCGGGTCGGACGTTGCATCGACAACGCCCCGATCGAGAGTTTTTGGGGCACCTTGAAAGTGGAGA
TGTACTACTTGCGTGAGTTCCAGGCCTATAGCGAACTCACAGCGGCCATCGAGAACTACATATCGTTCTACAACCACGATCGTCTCCAGAAACGACTGAA
CGGCTTGAGCCCTGTCGAATACAGGTCTCAAGCCGCTTAGGCTGGTTTTCATTATTTACCCTGTCTACTTGACGGGGAGCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 675 bp | 224 aa | 70 | 744 | + | No |
Description : First part of the transposase
ORF sequence :
MARSRHTIEQKLSALRMMEEETYTWKEIMEAHGVSRHTLQVWKVKFDTGGIDALKESKAWKLYTKELKIAAVRDYLDGFTRMEVLSKYQISDWSVLRKWI
KKYTSHSELTDSRKGMDRAMTKGRKTTFEERLEIVKYCLENGRNYQQTAEQFQVSYAQVYGWIKKYDTNGAEALVDRRGRTKPEDELSEEEKLKRRIQQM
DLENELLRADILFLKKLEEIERRS
KKYTSHSELTDSRKGMDRAMTKGRKTTFEERLEIVKYCLENGRNYQQTAEQFQVSYAQVYGWIKKYDTNGAEALVDRRGRTKPEDELSEEEKLKRRIQQM
DLENELLRADILFLKKLEEIERRS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 678 | 1640 | + | No |
Description : Second part of the transposase
ORF sequence :
ERTSSCGHLVSKKVRGDREEVMISQVRLQQRYIAIKELNEEEALSIVLLCRVAGISRAAYYKWLNRTIPVRQEENEKLLEDIRLLYDQANGTYGYRRITM
TINRLRRQQGLSPFNEKRVYRLMHIHGIQSVIRRKRKKHKKSTPQHVAENLMNREFSASRPDEKWCTDVTEFKYGAGKKAYLSAIIDLYDGSIVAYRIGK
SNNNALVFQTMMPAIAGLRTGARPMIHSDRGFQYTSRGFKRMVEDAGMTRSMSRVGRCIDNAPIESFWGTLKVEMYYLREFQAYSELTAAIENYISFYNH
DRLQKRLNGLSPVEYRSQAA
TINRLRRQQGLSPFNEKRVYRLMHIHGIQSVIRRKRKKHKKSTPQHVAENLMNREFSASRPDEKWCTDVTEFKYGAGKKAYLSAIIDLYDGSIVAYRIGK
SNNNALVFQTMMPAIAGLRTGARPMIHSDRGFQYTSRGFKRMVEDAGMTRSMSRVGRCIDNAPIESFWGTLKVEMYYLREFQAYSELTAAIENYISFYNH
DRLQKRLNGLSPVEYRSQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1571 bp | 523 aa | 70 | 1640 | + | Yes |
Chemistry : DDE
ORF sequence :
MARSRHTIEQKLSALRMMEEETYTWKEIMEAHGVSRHTLQVWKVKFDTGGIDALKESKAWKLYTKELKIAAVRDYLDGFTRMEVLSKYQISDWSVLRKWI
KKYTSHSELTDSRKGMDRAMTKGRKTTFEERLEIVKYCLENGRNYQQTAEQFQVSYAQVYGWIKKYDTNGAEALVDRRGRTKPEDELSEEEKLKRRIQQM
DLENELLRADILFLKKVRGDREEVMISQVRLQQRYIAIKELNEEEALSIVLLCRVAGISRAAYYKWLNRTIPVRQEENEKLLEDIRLLYDQANGTYGYRR
ITMTINRLRRQQGLSPFNEKRVYRLMHIHGIQSVIRRKRKKHKKSTPQHVAENLMNREFSASRPDEKWCTDVTEFKYGAGKKAYLSAIIDLYDGSIVAYR
IGKSNNNALVFQTMMPAIAGLRTGARPMIHSDRGFQYTSRGFKRMVEDAGMTRSMSRVGRCIDNAPIESFWGTLKVEMYYLREFQAYSELTAAIENYISF
YNHDRLQKRLNGLSPVEYRSQAA
KKYTSHSELTDSRKGMDRAMTKGRKTTFEERLEIVKYCLENGRNYQQTAEQFQVSYAQVYGWIKKYDTNGAEALVDRRGRTKPEDELSEEEKLKRRIQQM
DLENELLRADILFLKKVRGDREEVMISQVRLQQRYIAIKELNEEEALSIVLLCRVAGISRAAYYKWLNRTIPVRQEENEKLLEDIRLLYDQANGTYGYRR
ITMTINRLRRQQGLSPFNEKRVYRLMHIHGIQSVIRRKRKKHKKSTPQHVAENLMNREFSASRPDEKWCTDVTEFKYGAGKKAYLSAIIDLYDGSIVAYR
IGKSNNNALVFQTMMPAIAGLRTGARPMIHSDRGFQYTSRGFKRMVEDAGMTRSMSRVGRCIDNAPIESFWGTLKVEMYYLREFQAYSELTAAIENYISF
YNHDRLQKRLNGLSPVEYRSQAA
Blast result :
Comments
ISEnfa5 is 65% aa similar to ISSth1b.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
As a predicated composite transposon structure on plasmid pStrcfr in Streptococcus suis, two ISEnfa5 elements associated with multiresistance gene cfr might play a role in dissemination of this resistance determinant in the species of streptococci. These two ISEnfa5 elements and cfr in pStrcfr showed 99.9% identity to the corresponding cfr-carrying segment of plasmid pEF-01 in Enterococcus faecalis.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
As a predicated composite transposon structure on plasmid pStrcfr in Streptococcus suis, two ISEnfa5 elements associated with multiresistance gene cfr might play a role in dissemination of this resistance determinant in the species of streptococci. These two ISEnfa5 elements and cfr in pStrcfr showed 99.9% identity to the corresponding cfr-carrying segment of plasmid pEF-01 in Enterococcus faecalis.
References
1] Wang, Y., Schwarz, S., Wu, C., and Shen, J. (2013) Direct ISfinder submission.
2] Liu,Y., Wang,Y., Wu,C., Shen,Z., Schwarz,S., Du,X.D., Dai,L., Zhang,W., Zhang,Q. and Shen,J. (2012) Antimicrob. Agents Chemother. 56 (3), 1650-1654
2] Liu,Y., Wang,Y., Wu,C., Shen,Z., Schwarz,S., Du,X.D., Dai,L., Zhang,W., Zhang,Q. and Shen,J. (2012) Antimicrob. Agents Chemother. 56 (3), 1650-1654