ISPa47
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| Y | Pseudomonas aeruginosa | Pseudomonas aeruginosa Ps159 |
DNA section
IS Length : 1107 bp
Ends
IR Length : 16/19
IRL : TTAACCTGGCAAATAAATAGGGCATATTAGATAATCTGTCTCCATGAAAA
IRR : TTAACCCGGCAATCAAATACACCAAATCATGCTGCGTAGGCGATACGTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTCCGCAAC | TA | CTATTTCAAC | 2 |
DNA sequence
TTAACCTGGCAAATAAATAGGGCATATTAGATAATCTGTCTCCATGAAAATAGATGCCCGTAAACTCAGCCCCCAAGAACAACGTGAAAAGCGCTCCACG
GCCCTACGCATGCGTGAGCAGGGTTACACCTATAAGGCTATTGGCGAAGCGGTTGGTGTTCACCCCCGCACTATTGCTCACTGGGCGCAGGTCGCAGAAC
ATAAAGGCGAAAAGGCTGCCATTGCCGGCGGCCAGCGTGGTGTGCGCCAGGGTGATCGCCGCAGTTTGAGCTCCAGCCAGGAAGTGCTGATTCGCACCTT
GATGACCGATAAGATGCCCGACCAACTCAAGCTCGGCTTTGCGCTCTGGACGCGTGATGCGGTGCGAGAACTGATCCGCCAGCGCTGTGGTTTTCTCATG
CCGGTTCGAACGGTTGGTGAATACCTCAAGCGTTGGGGCTACACCCCGCAGCGCCCACTGCATCGGGCTTATCAGCAGAAACCTGAAGTGGTTCAGCACT
GGCTGGATAATGAATATCCACGCATCGCACAGCGGGCCAGGGCTGAGAATGGTGAGATTCAGTGGGGCGACGAAACCGGTATGCGCAGTGACAGCCATGC
TGGCCGCAGCTACGCCCCTATTGGCGAAACGCCGGTGCGCCTGGTCAGCGGCAGTCGTTTTTCCACCAACATGATTTCCACCGTGACCAATCGGGGCAAA
CTGCGCTTCATGCTGTATCGGGAAACGCTGACAGCCCCAGTGCTGATTCGCTTCCTGAGTCGCCTGATTCGCGATGCTCAGGGCCGCAAGGTGTTTCTGA
TTCTCGACAACCTGCGCGTACACCACAGCAAAAAGGTGAGCGCCTGGGTTGGCGACCGCAAAGAGCAAATCGAACTGTTCTTCTTGCCGGCCTACGCCCC
GGAGTTGAACCCTGACGAGTATTTGAATTGTGATTTGAAACATCAGGTTCGCACGGGCTTGCCGGCGCGTAATCAGGACGAACTGGAAAGGCGTGTTCGC
TCGGTCATGAGACGATTGCAATTACGCCCTCAAAGAATCCGTTCTTATTTCCGGCATCCACGTATCGCCTACGCAGCATGATTTGGTGTATTTGATTGCC
GGGTTAA
GCCCTACGCATGCGTGAGCAGGGTTACACCTATAAGGCTATTGGCGAAGCGGTTGGTGTTCACCCCCGCACTATTGCTCACTGGGCGCAGGTCGCAGAAC
ATAAAGGCGAAAAGGCTGCCATTGCCGGCGGCCAGCGTGGTGTGCGCCAGGGTGATCGCCGCAGTTTGAGCTCCAGCCAGGAAGTGCTGATTCGCACCTT
GATGACCGATAAGATGCCCGACCAACTCAAGCTCGGCTTTGCGCTCTGGACGCGTGATGCGGTGCGAGAACTGATCCGCCAGCGCTGTGGTTTTCTCATG
CCGGTTCGAACGGTTGGTGAATACCTCAAGCGTTGGGGCTACACCCCGCAGCGCCCACTGCATCGGGCTTATCAGCAGAAACCTGAAGTGGTTCAGCACT
GGCTGGATAATGAATATCCACGCATCGCACAGCGGGCCAGGGCTGAGAATGGTGAGATTCAGTGGGGCGACGAAACCGGTATGCGCAGTGACAGCCATGC
TGGCCGCAGCTACGCCCCTATTGGCGAAACGCCGGTGCGCCTGGTCAGCGGCAGTCGTTTTTCCACCAACATGATTTCCACCGTGACCAATCGGGGCAAA
CTGCGCTTCATGCTGTATCGGGAAACGCTGACAGCCCCAGTGCTGATTCGCTTCCTGAGTCGCCTGATTCGCGATGCTCAGGGCCGCAAGGTGTTTCTGA
TTCTCGACAACCTGCGCGTACACCACAGCAAAAAGGTGAGCGCCTGGGTTGGCGACCGCAAAGAGCAAATCGAACTGTTCTTCTTGCCGGCCTACGCCCC
GGAGTTGAACCCTGACGAGTATTTGAATTGTGATTTGAAACATCAGGTTCGCACGGGCTTGCCGGCGCGTAATCAGGACGAACTGGAAAGGCGTGTTCGC
TCGGTCATGAGACGATTGCAATTACGCCCTCAAAGAATCCGTTCTTATTTCCGGCATCCACGTATCGCCTACGCAGCATGATTTGGTGTATTTGATTGCC
GGGTTAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1038 bp | 345 aa | 44 | 1081 | + | No |
Chemistry : DDE
ORF sequence :
MKIDARKLSPQEQREKRSTALRMREQGYTYKAIGEAVGVHPRTIAHWAQVAEHKGEKAAIAGGQRGVRQGDRRSLSSSQEVLIRTLMTDKMPDQLKLGFA
LWTRDAVRELIRQRCGFLMPVRTVGEYLKRWGYTPQRPLHRAYQQKPEVVQHWLDNEYPRIAQRARAENGEIQWGDETGMRSDSHAGRSYAPIGETPVRL
VSGSRFSTNMISTVTNRGKLRFMLYRETLTAPVLIRFLSRLIRDAQGRKVFLILDNLRVHHSKKVSAWVGDRKEQIELFFLPAYAPELNPDEYLNCDLKH
QVRTGLPARNQDELERRVRSVMRRLQLRPQRIRSYFRHPRIAYAA
LWTRDAVRELIRQRCGFLMPVRTVGEYLKRWGYTPQRPLHRAYQQKPEVVQHWLDNEYPRIAQRARAENGEIQWGDETGMRSDSHAGRSYAPIGETPVRL
VSGSRFSTNMISTVTNRGKLRFMLYRETLTAPVLIRFLSRLIRDAQGRKVFLILDNLRVHHSKKVSAWVGDRKEQIELFFLPAYAPELNPDEYLNCDLKH
QVRTGLPARNQDELERRVRSVMRRLQLRPQRIRSYFRHPRIAYAA
Blast result :
Comments
ISPa47 is 66% aa similar to ISCARN44.
This insertion sequence has been found truncating the oprD gene of Pseudomonas aeruginosa Ps159 strain. This inactivation of the oprD gene could be the cause of the carbapenem resistance phenotype in this strain.
This insertion sequence has been found truncating the oprD gene of Pseudomonas aeruginosa Ps159 strain. This inactivation of the oprD gene could be the cause of the carbapenem resistance phenotype in this strain.
References