ISAmde2

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013385 ND Ammonifex degensii
Ammonifex degensii KC4
DNA section
IS Length : 1584 bp

Ends


IR Length : 19/25

IRL : GTTAGTGTAAAGTTACTATAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAA
IRR : GATCGTGTCAACCTACTGTAGGAGTCTCGATCCCCACCGTCCCTGAGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
TAGCGCAAAACCCGGAGCCGAGTGGTAGT10
ATTATGCTCCGGGGAGGTTCACAACAGTT9
TTGACACAGACCTAACACGGGGTGTATTG9
GGACGGAGACCGGGCTTGCCCAGGTT6

DNA sequence

GTTAGTGTAAAGTTACTATAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAACGAAGAGAGACCTCACCGTCCTTCGGAGGGACGAGCAGTTGACAATCCAA
CCACTCAGGAGGGTGAGGTCTCTATGCAGATTATAAAGCAGATTTGGGAGAAGTTCCAGAGGGTAGCGGAGCTAACGTTAGAGGTTCTGGAGGAAGGGAT
CGACCTTTTAAGCTTCGAGGAGAAGCTCTGGCAGGAGCTTAAAAGCTTAGGAAAAGAGATCCTGAGGGAAGTTCTGGAGGCAAAAGACGCTTATTTACGG
GAGCACCGGGAAAAGCGGCCTGGCTGGCAAGTAGAGCGGAGGAATGATCCCAAGGAAGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTATT
ACCGGCACAAAGAGACTGGGGAGCGGGCATACTTAATAGACCGTTTGGTGGGTTACGGGCCGCATACGCGGGTTGACCCTCTGGTCAAGGCACAAGTTTT
AGAAGAAGCAGTAGAGCTTTCTTACCGGAAAAGTGGGGAAAGGGCAGGAAAAGGCAACCCGGAGGTTGTGCTGAGTGGTGAGGCTGTGAAAAAGGTAGTT
CACAACTTCACCCCTGCAGAACTGCCCGAACCACCCAAAGAGAAGCGCCGGGTGAAGGTCCTTTACGTAGAAGCGGACGAAGACCACGTAGCTGGCCAGG
ACAAGAAGAGCCACCTACCTCGTCTTGTTTACATACACGAAGGGAAAGAAGAGGTGGGGAAAGGGCGGTATAAGCTTAAGCGGCCTTACTACCTGGGAGG
GCTTTATCCGGACACGGATGAACTGTGGCTGGATGTTCTAACTTACATTGAGGAGCATTATGAGCTACATGATATTGAGCGGATCTATCTTTGTGGAGAC
GGGGACAGGTGGATAAAGAGGGGTCTGGAGTTTTTACCGAAGAGCGTATTTGTTCTGGACCTTTTCCACCTGGATAAATACCTTGTGGCCGCTTTAGGAA
AGGACAAAGAGGCATATGGAGAGATTTGGGCAGCCTTGAGGCGTGGTGATCGGGTGGGGGTAGAGAAGGTACTGAAGGGAGCGGCGAGGCAGGCGGAGAC
ACCTGGCCGGAGGAAGGCGGTGCGTGATTGTCGGCGCTACATAAATCAGAACTGGGAGGGGATAATGGCTTACAAGCTTTACCCGGAGGCGCAGTTAGGG
GTGAGCGCGGAGGCGCACGTAAGTCATCTCTTCTCGGCACGGTTATCATCCCGACCGATGGCTTGGAGTGCTTGTGGGGTAGACCGGATGGCCCGGTTGC
GGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAGGA
AGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCATTACGGGGTCCGGTAACGTCATTAACCAGAGCCCTCAAAGCTTTG
AGCCGGAACATCGATCTTCCCTGGTAGTTCGTCCTGGCTCAGGGACGGTGGGGATCGAGACTCCTACAGTAGGTTGACACGATC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1404 bp467 aa1241527+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQIIKQIWEKFQRVAELTLEVLEEGIDLLSFEEKLWQELKSLGKEILREVLEAKDAYLREHREKRPGWQVERRNDPKEVLTLFGLVSYKRTYYRHKETGE
RAYLIDRLVGYGPHTRVDPLVKAQVLEEAVELSYRKSGERAGKGNPEVVLSGEAVKKVVHNFTPAELPEPPKEKRRVKVLYVEADEDHVAGQDKKSHLPR
LVYIHEGKEEVGKGRYKLKRPYYLGGLYPDTDELWLDVLTYIEEHYELHDIERIYLCGDGDRWIKRGLEFLPKSVFVLDLFHLDKYLVAALGKDKEAYGE
IWAALRRGDRVGVEKVLKGAARQAETPGRRKAVRDCRRYINQNWEGIMAYKLYPEAQLGVSAEAHVSHLFSARLSSRPMAWSACGVDRMARLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLPW

 

Blast result :
Comments
ISAmde2 is 60% aa similar to ISNth2.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References