ISBsa1
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_016943 | ND | Blastococcus saxobsidens | Blastococcus saxobsidens DD2 |
DNA section
IS Length : 1465 bp
Ends
IR Length : 31/37
IRL : GAGGTTGTCCCGGGTCTCGTAGAAGTTGAGGTGGCGGTCCCCCGCCCGCA
IRR : GAGGATGTCCCGAGTTCCGTAGAACTTCGGTGGCGGCCCGTTGTGCGTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCGCGCCAGGGCGGGC | CTCGTCGTGC | 0 | |
| TCGTGCTCATCGCCTGCAAC | GGGCGGGCAAGTCGCGGTCG | 0 | |
| CCGTGCTCAGCCCTCGTCGT | CCTGCAACGCAGTGGGCCAAC | 0 | |
| GGCGGCGATCG | CCCCGCCGA | TGAACGTGCGGC | 9 |
| GCCCTTCGAGCA | GCAGTCCG | CGGCCGCCGGAT | 8 |
| GACCGAGCCGGC | TTTCCTTG | GGTCACCTCGCA | 8 |
DNA sequence
GAGGTTGTCCCGGGTCTCGTAGAAGTTGAGGTGGCGGTCCCCCGCCCGCACCGGCCGTGAGGTAGGTGTTGGGCGTCCGCCAAGACACCACTTCGAGCAG
GGGACCGCCGTGACCAAGAAGTACCAGAAGGACAAGATCGACACGCCTACCGGCGACGGAGTCGTCGTGCCGGAGCGGGTGAATGTGGCGATGGCCGAGA
TCGCCGGCACGATGCGTGAGGGCCTGCTCGCCCTCGCGGTCGGCGCCGGCCTGCAGGTGATGCAGGCGCTGATGGAGGCCGACGTGACCGGGATGGCCGG
GCCGAAGGGCAAGCACGATGCCGGTCGGACCGCGGTCCGGCACGGCTCCGAGCGCGGGTCGGTGACCCTGGGCGGGCGGCGGGTGCCAGTGAGCCGTCCG
CGGGTGCGCGCCGCTGACGGCTCCGGCGAGCTGTCCGTGCCCACCTACGAGCTGTTCAGCGGCACCGAGATCCTCGGGTCGATGGCGATGGAGCGGATGC
TGGCCGGCCTGTCCACCCGCCGCTACCGGGTTGGCCTGGAACCGGTCGGCGACCACGTCAGCGCGGCGGCGAAGGCGACGAGCAAGTCGGCGGTGTCCCG
GAAGTTCGTGGCGATGACCGAGACCGCCCTGGCCGAGCTGCTTGCCGCGGACCTGTCCGCATTGGACCTGGTCGCGCTGATGGTCGACGGGGTGCACTTC
GGCGAATCCTGCTGCGTCGTCGCGCTGGGCATCGACTCCGAGGGGGTCAAGCACCCCCTCGCCCTGGTCGAGGGGGCGACGGAGAACGCCACGGTGGTCA
CCGACCTGCTGGTCGGCCTGCGCGATCGGGGGCTGGACGTGAGCCGGCCGATCCTGGTCGGCATCGACGGGGCGAAGGCGCTGCGCAAGGCCGTGGTCGA
CGTCTTCGACCACCCGGTGATCCAGCGCTGCCAATTGCACAAGATCCGAAACGTTCAGGACCGACTACCGCAGCGGCTACGCGGCCCGGTCGGCACGAAG
ATGCGCGCGGCCTACCACGCCGACTCGGCGCTAGAGGCCGAGGCGGCGCTGACCGGGCTGGCCCGGGAGCTGGACAAGACCCACCCGAGCGCGGCTGCCA
GCCTGCGCGAAGGCCTGCACGAGACGCTGACCGTGCTGCGCCTGGGGGTGCCGCCCACGCTGGCCCGCACGCTGCGCTCGACCAACGCGATCGAGTCGAT
GATCTCCATCTGTCGTGATCACGCCCGCAACGTCAAGCGCTGGCGGGACGGGCAGATGGCCCTGCGCTGGTGCGCCGCCGGCATGGTCGAGGCCGGCAAG
CAGTTCCGCCGGGTCAACGGCCACCTGCACCTGGCGAAGCTGCGCGCCGCGCTCGACGCCGAGATCACCGGAACTGTCACACCCGCCGTGCATGATGAGG
AGGTCGTCGCAGCCTGACGCACAACGGGCCGCCACCGAAGTTCTACGGAACTCGGGACATCCTC
GGGACCGCCGTGACCAAGAAGTACCAGAAGGACAAGATCGACACGCCTACCGGCGACGGAGTCGTCGTGCCGGAGCGGGTGAATGTGGCGATGGCCGAGA
TCGCCGGCACGATGCGTGAGGGCCTGCTCGCCCTCGCGGTCGGCGCCGGCCTGCAGGTGATGCAGGCGCTGATGGAGGCCGACGTGACCGGGATGGCCGG
GCCGAAGGGCAAGCACGATGCCGGTCGGACCGCGGTCCGGCACGGCTCCGAGCGCGGGTCGGTGACCCTGGGCGGGCGGCGGGTGCCAGTGAGCCGTCCG
CGGGTGCGCGCCGCTGACGGCTCCGGCGAGCTGTCCGTGCCCACCTACGAGCTGTTCAGCGGCACCGAGATCCTCGGGTCGATGGCGATGGAGCGGATGC
TGGCCGGCCTGTCCACCCGCCGCTACCGGGTTGGCCTGGAACCGGTCGGCGACCACGTCAGCGCGGCGGCGAAGGCGACGAGCAAGTCGGCGGTGTCCCG
GAAGTTCGTGGCGATGACCGAGACCGCCCTGGCCGAGCTGCTTGCCGCGGACCTGTCCGCATTGGACCTGGTCGCGCTGATGGTCGACGGGGTGCACTTC
GGCGAATCCTGCTGCGTCGTCGCGCTGGGCATCGACTCCGAGGGGGTCAAGCACCCCCTCGCCCTGGTCGAGGGGGCGACGGAGAACGCCACGGTGGTCA
CCGACCTGCTGGTCGGCCTGCGCGATCGGGGGCTGGACGTGAGCCGGCCGATCCTGGTCGGCATCGACGGGGCGAAGGCGCTGCGCAAGGCCGTGGTCGA
CGTCTTCGACCACCCGGTGATCCAGCGCTGCCAATTGCACAAGATCCGAAACGTTCAGGACCGACTACCGCAGCGGCTACGCGGCCCGGTCGGCACGAAG
ATGCGCGCGGCCTACCACGCCGACTCGGCGCTAGAGGCCGAGGCGGCGCTGACCGGGCTGGCCCGGGAGCTGGACAAGACCCACCCGAGCGCGGCTGCCA
GCCTGCGCGAAGGCCTGCACGAGACGCTGACCGTGCTGCGCCTGGGGGTGCCGCCCACGCTGGCCCGCACGCTGCGCTCGACCAACGCGATCGAGTCGAT
GATCTCCATCTGTCGTGATCACGCCCGCAACGTCAAGCGCTGGCGGGACGGGCAGATGGCCCTGCGCTGGTGCGCCGCCGGCATGGTCGAGGCCGGCAAG
CAGTTCCGCCGGGTCAACGGCCACCTGCACCTGGCGAAGCTGCGCGCCGCGCTCGACGCCGAGATCACCGGAACTGTCACACCCGCCGTGCATGATGAGG
AGGTCGTCGCAGCCTGACGCACAACGGGCCGCCACCGAAGTTCTACGGAACTCGGGACATCCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1308 bp | 435 aa | 110 | 1417 | + | No |
Chemistry : DDE
ORF sequence :
MTKKYQKDKIDTPTGDGVVVPERVNVAMAEIAGTMREGLLALAVGAGLQVMQALMEADVTGMAGPKGKHDAGRTAVRHGSERGSVTLGGRRVPVSRPRVR
AADGSGELSVPTYELFSGTEILGSMAMERMLAGLSTRRYRVGLEPVGDHVSAAAKATSKSAVSRKFVAMTETALAELLAADLSALDLVALMVDGVHFGES
CCVVALGIDSEGVKHPLALVEGATENATVVTDLLVGLRDRGLDVSRPILVGIDGAKALRKAVVDVFDHPVIQRCQLHKIRNVQDRLPQRLRGPVGTKMRA
AYHADSALEAEAALTGLARELDKTHPSAAASLREGLHETLTVLRLGVPPTLARTLRSTNAIESMISICRDHARNVKRWRDGQMALRWCAAGMVEAGKQFR
RVNGHLHLAKLRAALDAEITGTVTPAVHDEEVVAA
AADGSGELSVPTYELFSGTEILGSMAMERMLAGLSTRRYRVGLEPVGDHVSAAAKATSKSAVSRKFVAMTETALAELLAADLSALDLVALMVDGVHFGES
CCVVALGIDSEGVKHPLALVEGATENATVVTDLLVGLRDRGLDVSRPILVGIDGAKALRKAVVDVFDHPVIQRCQLHKIRNVQDRLPQRLRGPVGTKMRA
AYHADSALEAEAALTGLARELDKTHPSAAASLREGLHETLTVLRLGVPPTLARTLRSTNAIESMISICRDHARNVKRWRDGQMALRWCAAGMVEAGKQFR
RVNGHLHLAKLRAALDAEITGTVTPAVHDEEVVAA
Blast result :
Comments
ISBsa1 is 86% aa similar to IS1295.
There are possibilities of recombination between two insertion sites of this IS.
There are possibilities of recombination between two insertion sites of this IS.
References
1] ISfinder annotation (2013)
2] Chouaia,B., Crotti,E., Brusetti,L., Daffonchio,D., Essoussi,I., Nouioui,I., Sbissi,I., Ghodhbane-Gtari,F., Gtari,M., Vacherie,B., Barbe,V., Medigue,C., Gury,J., Pujic,P. and Normand,P. (2012) J. Bacteriol. 194 (10), 2752-2753.
2] Chouaia,B., Crotti,E., Brusetti,L., Daffonchio,D., Essoussi,I., Nouioui,I., Sbissi,I., Ghodhbane-Gtari,F., Gtari,M., Vacherie,B., Barbe,V., Medigue,C., Gury,J., Pujic,P. and Normand,P. (2012) J. Bacteriol. 194 (10), 2752-2753.