ISfinder Mirror Site

ISSag11

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AEXT01000002	ND	Streptococcus agalactiae	Streptococcus agalactiae FSL S3-026

DNA section

IS Length : 1313 bp

Ends

IR Length : 22/29

IRL : GGTAGTGTAAAATATCTTGTGTAAACACAAAAAGGAATAAATCCTGTATA
IRR : GGAGTCTGTCAATAATCTTGTGTAAACACTCTAGCAAAGCTGATATTGTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCGTCTACTC	ATTTCTT	TTGATAAAAC	7
GTGATAAATC	ATATATT	GGAATAGCTA	7
ATGTAAAAAC	TGTTACA	GGGAGGTTGA	7

DNA sequence

GGTAGTGTAAAATATCTTGTGTAAACACAAAAAGGAATAAATCCTGTATAGTAGAGTTGCGAGACTTCACTAGAAAGAGATTTATTCCCATGACCCAGTT
TACCACAGAATTACTTAACTTCCTAGCTCAAAAACAAGATATTGATGAATTTTTCCGTTCGTCTCTTGAAACTGCCATGAATGACCTTCTCCAAGTGGAG
CTATCCGCCTTCCTTGGCTATGAACCATACGATAAGGTAGGTTACAACACCGGTAATAGTCGTAATGGTGCTTATACACGTCGATTTGAGACCAAGTATG
GTGTTGTTAATTTGTTGATCCCTAGAGATCGAAACGGGGAGTTCAGTCCAGCTTTAATCCCAAGTTACGGTCGTCGAGACAATCATTTAGAAGAAATGGT
TATCAAGCTTTATCAAACTGGAGTCACAACACGTGAAATCAGCGACATCATTGAGCGTATGTATGGTCATCATTACAGTCCAGCAACAGTATCTAATATC
TCGAAAGCGACACAGGAAAACGTGGCTAGCTTTCATGAACGTTCCTTAGAAGCTAACTATACCGTATTATATCTTGATGGGACTTACCTTCCTCTGAGAC
GTGGTACAGTTAGTAAGGAATGCATCCACATTGCACTAGGCGTCACATCATATGGGCATAAGGCTATCCTTGGATATGACATCGCACCCAATGAAAACAA
TGCTTCGTGGTCAGACCTTCTAGAAAGACTTAAGGGTCAAGGTGTACAACAAGTCTCTCTTGTTGTGACTGATGGTTTTAATGGACTTGATCAGCTTATC
CAGCAAGCCTTCCCAATGGCCAAACAACAGCGTTGCCTTGTCCATATTGGCCGAAATATTGCAAGTAAGGTGAAACGAGCAGATCGTGCTCTAATTTTGG
AGCAGTTTAAAACAATTTATCGCGCGATCAATGTAGAGGAAGCAAAGCAAGCCTTAGATAGCTTTATCAATGAGTGGAAACCACACTACAAGAAGGTAAT
AGAGACCTTAGAATCAATCGAAAATCTATTGATCTTTTATGAATTTCCTCATCAGATCTGGGGAAGTATCTACTCGACCAACCTTATTGAATCACTTAAC
AAAGAAATCAAACGTCAAACCAAAAAGAAAGTCGTCTTTCCCAATGAAGAATCCCTAGAACGCTATCTCGTCACACTATTTAGTGATTACAACTTCAAGC
AAGGACAACGAATCCACAAAGGTTTTGGCCAATGTACGGACACACTTGAAAGCCTATTTGATTAACAATATCAGCTTTGCTAGAGTGTTTACACAAGATT
ATTGACAGACTCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1176 bp	391 aa	90	1265	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTQFTTELLNFLAQKQDIDEFFRSSLETAMNDLLQVELSAFLGYEPYDKVGYNTGNSRNGAYTRRFETKYGVVNLLIPRDRNGEFSPALIPSYGRRDNHL
EEMVIKLYQTGVTTREISDIIERMYGHHYSPATVSNISKATQENVASFHERSLEANYTVLYLDGTYLPLRRGTVSKECIHIALGVTSYGHKAILGYDIAP
NENNASWSDLLERLKGQGVQQVSLVVTDGFNGLDQLIQQAFPMAKQQRCLVHIGRNIASKVKRADRALILEQFKTIYRAINVEEAKQALDSFINEWKPHY
KKVIETLESIENLLIFYEFPHQIWGSIYSTNLIESLNKEIKRQTKKKVVFPNEESLERYLVTLFSDYNFKQGQRIHKGFGQCTDTLESLFD

Blast result :

Comments

ISSag11 is 93% aa similar to IS905.

References

1] ISfinder annotation (2013)
2] Richards,V.P., Lang,P., Pavinski Bitar,P.D., Lefebure,T., Schukken,Y.H., Zadoks,R.N. and Stanhope,M.J. (2011) Infect. Genet. Evol. 11 (6), 1263-1275.