ISfinder Mirror Site

ISVra1

Family IS256
Group IS1249

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_JH992938	ND	Veillonella ratti	Veillonella ratti ACS-216-V-Col6b

DNA section

IS Length : 1329 bp

Ends

IR Length : 19/21

IRL : GGACATTTTGAGTTGGTTTTTAGACCGATGAGACTAGTATTTATCTAGTC
IRR : GGACATTTTGAGTTGGTAATTTAATCCCAAAGATAGTTAGGATATGGATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAGTTTGGCC	TATA	ACCCTAAATT	4

DNA sequence

GGACATTTTGAGTTGGTTTTTAGACCGATGAGACTAGTATTTATCTAGTCTTGTCGGTCTTTTAAATTTTTAATTAGATTTTAAAGGGTGGACAAAATGT
GTTGGTAAAAAGTCTGAAACCCCTTGCTATATAAGGGTTAAATAAGGTTATATCTTTCTAGAAAATATATTTTTTAGGAGGTATTACTTTATGAGAAAAA
TTAAGTGTCCTTATTGTGGGTTTGACTGTATAAAATATGGAAAAGCTCGAATAGGTAGCCAACGATGGCGTTGTAGAGAATGTCATCAAGTTTTTACTAA
ACCTTATGATCATACAAACTCTGACTTTCGGCTTTTTCTTCAGTGGTTGTTCAGTAAAAATGTACAAAAAGATATGCCCGGTGTCGGTCGTAATTTTCGC
AGAAAAACAGTTAAATTCTGGGAACTTTGGCCTTTGTTACCCAAAGTTGAAACTCAACGCTCGATTGTATTCATTGATGGTATTCACCTTGGAAGAAAGG
CCTGTATATTAATCTGTTGTGATGAAAAGCATGTGTTAGGTTGGTATGTATGCCGAGCTGAACGAGCGCAGGCTTGGGAGGCTTTGATGAGCCGTATTGC
TACGCCTAAAGTCGTTGTATCTGATGGTGGTACCGGATTTCGGAAAGCATTAAAAAAAGTTTGGCCAAAGGCCAAACTTCAAAGATGTATTTTTCATGCT
TCTCAGCAAGTAAAAAGATATACGACAACCCGTCCTAAAACACTTGCCGGTGCAGAGCTATATGGGCTTTCAAATGACTTATTTAAGGTAAATTCAACAG
ATGCAGCATTAAATTGGGTTAGGCGTTTAACAGACTGGAAAATTAAGCATAAAGCCTTTTTAGATGAAGTATCTTATGACGAGTACGGAAAACCTAGATT
AAAACATGAGAGACTTTTAAAAGCAGAGCAGTCACTCATTGTATTAATAAAATCCGGTACCTCATTCACTTACCTAGATGAGACATTAGACTTTGACTGT
TCATCTACTAATAACCGTATTGAAGGCGGCATAAATGCTCAATTAAGAGCTATGTTGCGTTACCATAGAGGGATGAATATTGAAAGACGAATAAAAGCGG
TTTTCTGGTGGTGCTACATGCACTCACCGAAACCGCTTTCACCCGCTGAAATACTCGAGGTTATGCCGACCAACAAAAGTATTTCTCAACTATATGATAC
CATGAATTCTAGAGAAAGATTAGAAAATTCTATCCCAACTTGGGGTGATGCTATTGTATGGAGTGATTTTCATTTCTCTGATCCATATCCTAACTATCTT
TGGGATTAAATTACCAACTCAAAATGTCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1119 bp	372 aa	191	1309	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIKCPYCGFDCIKYGKARIGSQRWRCRECHQVFTKPYDHTNSDFRLFLQWLFSKNVQKDMPGVGRNFRRKTVKFWELWPLLPKVETQRSIVFIDGIHL
GRKACILICCDEKHVLGWYVCRAERAQAWEALMSRIATPKVVVSDGGTGFRKALKKVWPKAKLQRCIFHASQQVKRYTTTRPKTLAGAELYGLSNDLFKV
NSTDAALNWVRRLTDWKIKHKAFLDEVSYDEYGKPRLKHERLLKAEQSLIVLIKSGTSFTYLDETLDFDCSSTNNRIEGGINAQLRAMLRYHRGMNIERR
IKAVFWWCYMHSPKPLSPAEILEVMPTNKSISQLYDTMNSRERLENSIPTWGDAIVWSDFHFSDPYPNYLWD

Blast result :

Comments

ISVra1 is 77% aa similar to ISVesp1.

References

1] ISfinder annotation (2013)
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Saerens,B., Vaneechoutte,M., Walker,B., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Chapman,S.B., Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J., McCowen,C., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B. (2012) Direct submission GenBank.