ISAnhy1

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_ACJX02000009.1 ND Anaerobaculum hydrogeniformans
Anaerobaculum hydrogeniformans ATCC BAA-1850
DNA section
IS Length : 1571 bp

Ends


IR Length : 22/28

IRL : GATCGCGTAAAATATTTGTAGGAGATTTTCAGAGGTAAAAAGAAAGGGAC
IRR : GACCGCGTCAAGTTTTTGTAGGATTTTTAGGAACCACTCCCCATGTGTCC

Insertion site


Left flankDirect repeatRight flankDR Length
TTGCGGTCATGGAAAAGACT0
GGGGGTATAGGAGAACAAACTTCTGCTTG9

DNA sequence

GATCGCGTAAAATATTTGTAGGAGATTTTCAGAGGTAAAAAGAAAGGGACCCCTCCTTTTGGTAGAATCTTTGTAGCACACAAAAACTCATAAAGGAGGG
TCCCAACTTGAACAAGGATATTTTACTACAAATAGCAATAAACTTCATCAAGGAGTTGCTTGAATTCTTCGGTGATTCCGAGGTCAGGACATTAGCGGAA
ATTGAAGACGAAATCTCTCGGATCATGAAGGCCTTCATCCGAGAACTGATAAAGGCTTACTTCGAGCTTGCAGATGAGGCCATATTAAAGGACAAAACAT
CTCGAAAGGAAAGAGGGTTAGTCGTAGAAAGGCGTGAGGACAAAAGAAGCGTTTATACCATCTTTGGGGATATATCTTTTAGCAGGACCTATTATTACGA
CAAAAAGCGAAACGCGTACATCTACCCCCTGGACAAAGCATTAGGGTTGGATAAATACGAAAGGCTGACTGAAGCGGTGGCCATAAAGCTGGTAGAAACG
GCAGGACAGGTATCTTACGCCAAAAGCTCGATGAACGTAACTAATGGCGAGTTATCCAGGCAGACCGTCAAAAACAAGATACACAACCTAAACCTTGAGG
CCTTGAAGACAAAAGTTCCTGAGAAAAGAAGCGTAAATGTATTACATATAGATGCGGATGAGGACCACGCATCGTTACAAGACGGCAGAAGCACGAATCT
TCCTTTGATCTGCATATACGAGGGCACCTTTAAAGAGGGATCCAAAAACAGATGTATAAACCCCATATACATGAGCGGCTATGGAAAGGGCAGCGATGAG
TTTTGGCTTGAGGTAACAGATAAGATATACGAGCTTTATGACCCGGAGGACATAAAGGGCATATACATCCATGGAGATGGTGCAAATTGGATAAAGGAAG
GGATAAACTGGCTGCCCGAGTCAAAGCTGGTGCTGGATAAATTCCACCTAAATAAAGCAATCCTGGAAGCCACGGCAAGGCAGCCCGAAAAAAGACGTGA
TATATACAGGGCCATAAATACAAGTGACTTGAACTTGTTTAAGAAAATAACCACCGAGATGGCTAAATGTGCTTCAAGCGAGAGGGAAAGAAAGATGATC
GCAGATTTTAGGAGATATATAACCAACAACTGGGGCTCCATTACCATTCGAAACGAAGAGGACTGCGGGAGCTCAAGTCCCGAGGGTCATATTAGCCACG
TACTTTCCTCTAGGTTAAGTTCTAGGCCTATAGCATGGAGCCGCAAGGGCTTAAAGGCCATGTCTTCTTTGAGGGCTTACATCTGTAGCGGTGGCAAGGT
GACGTCAGAACAGATAAAGAAAAAAGACCAAAAAGGTGAGAATGCGGACAAAAGACACAAATTCACCTTGAACCTGGAGGAGATCTTAGGTCCCGTTATA
AGTGAGCTTGGATGCATAACGGTACTGAAAACGGGAAAGGTAACGCCCTTATACACGACCTTAAGGGGCATATGTCATGGCGGGTTTGATTTTTACCTAA
CCTAAAAGACGTTGCCCTTTAGGACACATGGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1404 bp465 aa1021505+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNKDILLQIAINFIKELLEFFGDSEVRTLAEIEDEISRIMKAFIRELIKAYFELADEAILKDKTSRKERGLVVERREDKRSVYTIFGDISFSRTYYYDKK
RNAYIYPLDKALGLDKYERLTEAVAIKLVETAGQVSYAKSSMNVTNGELSRQTVKNKIHNLNLEALKTKVPEKRSVNVLHIDADEDHASLQDGRSTNLPL
ICIYEGTFKEGSKNRCINPIYMSGYGKGSDEFWLEVTDKIYELYDPEDIKGIYIHGDGANWIKEGINWLPESKLVLDKFHLNKAILEATARQPEKRRDIY
RAINTSDLNLFKKITTEMAKCASSERERKMIADFRRYITNNWGSITIRNEEDCGSSSPEGHISHVLSSRLSSRPIAWSRKGLKAMSSLRAYICSGGKVTS
EQIKKKDQKGENADKRHKFTLNLEEILGPVISELGCITVLKTGKVTPLYTTLRGICHGGFDFYLT

 

Blast result :
Comments
ISAnhy1 is 59% aa similar to ISTte3.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References