ISNpe8
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP003372 | ND | Natrinema pellirubrum | Natrinema pellirubrum DSM 15624 Natrinema pellirubrum DSM 15624 plasmid pNATPE01 |
DNA section
IS Length : 1650 bp
Ends
IR Length : 14/15
IRL : GTACGTGTTTAGCCCCGTCTGATTTCGTCACTGTCTCGTAGGTGGTGGCC
IRR : GTACGTGTTTACCCCGAGGTCTCAACAACCGGCACAGTCTGCTCTCGTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATTACGATA | GTATTATT | TATATATACT | 8 |
| CACGGCGTTG | ACCTGATA | GAGCGCGAGC | 8 |
| TTCTGGCTTA | GAAAATCT | TTACCCTGTA | 8 |
| GGCTGATTTT | GAATAGTT | TTTAGAACAC | 8 |
| CGACATACTG | ATCTTCCC | ACTCCCAACG | 8 |
| GTCACATTTC | GGTATCTG | AATAGCATTA | 8 |
| CCACTCAAAA | GAAATATC | CAGGCGTCCT | 8 |
| GCGGTAACTA | CAGATGCT | TATAGAACGG | 8 |
| GAAGCGAAAT | CG | ACAACGAAAT | 2 |
DNA sequence
GTACGTGTTTAGCCCCGTCTGATTTCGTCACTGTCTCGTAGGTGGTGGCCTGTGACAGATACATGGTCTGTCAGCAGGGAGTCAGTCTACATTCTCAGGG
ATGGTCTCCGCTGCCCGGAGACCATCCCGTCGGCCCTGCTGTCGTCGTTTCGAGGTGGAATCGATGAGCTGGGACGATCTCTCCAAGGACGAACTCCTCT
CGCGGTTTCTCCAGATGGAGGAGCGAATCGACGAGCTGGAAGAGAAGATTGCCCAGAAGGACAAGCGGATCGAGGAACAGAACGAACGGATCGAAGAGCA
GCAAGAGCGGATCGAAGAGCAGCAAGAGCGGATCGAAGAACTCGAAACACGTCTCCGGAAGTACGAGAATCCACACACTCCACCCAGTAAGCGACGGTCG
GGGACCGACGAGTCCCCGACCTCGCAGGACGACGAAGACGACGATGTCCGAACTGATGGTGGTACTCCCGGTCGAAAGGACGGTCACAACCCGGAGTGGC
GCTCTCCGCCCGATCCTGATGAAGAAATCGAGGTCACCTCTGACTGTTGTCCCGAGTGTGGCGACCACTTCGACGAGTCGGTGGGCGTCAGCCCCCGACT
CGTCGAGGAAGTCCCGGATCCACAGCCACCAGAAGTCACCCAGTACAACCGACACCGCTACCAATGCGACTCCTGTGGAACTGAGACGGTTGCGACTCAC
CCCGACTGCCCCGATGAGGGGCAGTTCGGGGTGAACGTCATCGCGCAATCTGCCCTGTCACGGTACGATCACCGCCTTCCCTACCGGAAAATCACGGACC
GCTTCGAGCAACTTCACGGACTCGAACTCTCAGGTGCATCCGCGTGGCACGCGACCGAGCGCGCTGCGCGCGCCGGTCGCTGTGAGTACGAGCAGATCCG
TCGAGAGATTCAGGAGGCTGAAATCGTCCACGTTGACGAAACCGGCATCAAGCGTGACGGTGACCAAGCGTGGATCTGGACGTTTCGGACGGACGAGCAT
ACGCTGTACGCGGTCAGAGAGAGTCGTGGGAGTGATGTTCCCGCGGAAGTCCTTGGCGAGGACTTCGCGGGAACGGTCATCTGTGATGGGTGGACGGCGT
ATCCAGCGTTCAGCAGTACGCTTCAGCGGTGCTGGGCACATCTTCTCCGGGAGGCTGAAGACGTTGCTAGTGACCACGAGGAGGCAGAACCGGTGTACCG
GTATCTCAAGCAGATGTTCGTCGGTCTCCAGTCGTGGCTGGAGACCGACCCAGATCCTCGTGCGAGAGCACAGATGCATCGAGCGTGTCAGGACGGCCTC
AGATCGCTCGTTGAGCGGTCAGTGACCGACGAACCAGTGGCAACACTCCTCGGGAAGATCGAAGGTGGACTCGACCACTGGCTCACCTTCGTCGGTGAGC
CAGCGGTCTCCCCGACGAACAATGCCGCAGAAAACGCCCTTCGTGAACCTGTTGTTCTCCGGAAAATCATCGGAACACTCCGGAACGACCGTGGCATGTT
CGTTCACGAGACGATCTTGTCCCTGCTGGCGACGTGGCGCCAGCAGGGACGCAACCCATACGAGGAACTTACGCGGGTTGTCCACGACAACGAAATGATC
TCACGAGAGCAGACTGTGCCGGTTGTTGAGACCTCGGGGTAAACACGTAC
ATGGTCTCCGCTGCCCGGAGACCATCCCGTCGGCCCTGCTGTCGTCGTTTCGAGGTGGAATCGATGAGCTGGGACGATCTCTCCAAGGACGAACTCCTCT
CGCGGTTTCTCCAGATGGAGGAGCGAATCGACGAGCTGGAAGAGAAGATTGCCCAGAAGGACAAGCGGATCGAGGAACAGAACGAACGGATCGAAGAGCA
GCAAGAGCGGATCGAAGAGCAGCAAGAGCGGATCGAAGAACTCGAAACACGTCTCCGGAAGTACGAGAATCCACACACTCCACCCAGTAAGCGACGGTCG
GGGACCGACGAGTCCCCGACCTCGCAGGACGACGAAGACGACGATGTCCGAACTGATGGTGGTACTCCCGGTCGAAAGGACGGTCACAACCCGGAGTGGC
GCTCTCCGCCCGATCCTGATGAAGAAATCGAGGTCACCTCTGACTGTTGTCCCGAGTGTGGCGACCACTTCGACGAGTCGGTGGGCGTCAGCCCCCGACT
CGTCGAGGAAGTCCCGGATCCACAGCCACCAGAAGTCACCCAGTACAACCGACACCGCTACCAATGCGACTCCTGTGGAACTGAGACGGTTGCGACTCAC
CCCGACTGCCCCGATGAGGGGCAGTTCGGGGTGAACGTCATCGCGCAATCTGCCCTGTCACGGTACGATCACCGCCTTCCCTACCGGAAAATCACGGACC
GCTTCGAGCAACTTCACGGACTCGAACTCTCAGGTGCATCCGCGTGGCACGCGACCGAGCGCGCTGCGCGCGCCGGTCGCTGTGAGTACGAGCAGATCCG
TCGAGAGATTCAGGAGGCTGAAATCGTCCACGTTGACGAAACCGGCATCAAGCGTGACGGTGACCAAGCGTGGATCTGGACGTTTCGGACGGACGAGCAT
ACGCTGTACGCGGTCAGAGAGAGTCGTGGGAGTGATGTTCCCGCGGAAGTCCTTGGCGAGGACTTCGCGGGAACGGTCATCTGTGATGGGTGGACGGCGT
ATCCAGCGTTCAGCAGTACGCTTCAGCGGTGCTGGGCACATCTTCTCCGGGAGGCTGAAGACGTTGCTAGTGACCACGAGGAGGCAGAACCGGTGTACCG
GTATCTCAAGCAGATGTTCGTCGGTCTCCAGTCGTGGCTGGAGACCGACCCAGATCCTCGTGCGAGAGCACAGATGCATCGAGCGTGTCAGGACGGCCTC
AGATCGCTCGTTGAGCGGTCAGTGACCGACGAACCAGTGGCAACACTCCTCGGGAAGATCGAAGGTGGACTCGACCACTGGCTCACCTTCGTCGGTGAGC
CAGCGGTCTCCCCGACGAACAATGCCGCAGAAAACGCCCTTCGTGAACCTGTTGTTCTCCGGAAAATCATCGGAACACTCCGGAACGACCGTGGCATGTT
CGTTCACGAGACGATCTTGTCCCTGCTGGCGACGTGGCGCCAGCAGGGACGCAACCCATACGAGGAACTTACGCGGGTTGTCCACGACAACGAAATGATC
TCACGAGAGCAGACTGTGCCGGTTGTTGAGACCTCGGGGTAAACACGTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1479 bp | 492 aa | 164 | 1642 | + | No |
Chemistry : DDE
ORF sequence :
MSWDDLSKDELLSRFLQMEERIDELEEKIAQKDKRIEEQNERIEEQQERIEEQQERIEELETRLRKYENPHTPPSKRRSGTDESPTSQDDEDDDVRTDGG
TPGRKDGHNPEWRSPPDPDEEIEVTSDCCPECGDHFDESVGVSPRLVEEVPDPQPPEVTQYNRHRYQCDSCGTETVATHPDCPDEGQFGVNVIAQSALSR
YDHRLPYRKITDRFEQLHGLELSGASAWHATERAARAGRCEYEQIRREIQEAEIVHVDETGIKRDGDQAWIWTFRTDEHTLYAVRESRGSDVPAEVLGED
FAGTVICDGWTAYPAFSSTLQRCWAHLLREAEDVASDHEEAEPVYRYLKQMFVGLQSWLETDPDPRARAQMHRACQDGLRSLVERSVTDEPVATLLGKIE
GGLDHWLTFVGEPAVSPTNNAAENALREPVVLRKIIGTLRNDRGMFVHETILSLLATWRQQGRNPYEELTRVVHDNEMISREQTVPVVETSG
TPGRKDGHNPEWRSPPDPDEEIEVTSDCCPECGDHFDESVGVSPRLVEEVPDPQPPEVTQYNRHRYQCDSCGTETVATHPDCPDEGQFGVNVIAQSALSR
YDHRLPYRKITDRFEQLHGLELSGASAWHATERAARAGRCEYEQIRREIQEAEIVHVDETGIKRDGDQAWIWTFRTDEHTLYAVRESRGSDVPAEVLGED
FAGTVICDGWTAYPAFSSTLQRCWAHLLREAEDVASDHEEAEPVYRYLKQMFVGLQSWLETDPDPRARAQMHRACQDGLRSLVERSVTDEPVATLLGKIE
GGLDHWLTFVGEPAVSPTNNAAENALREPVVLRKIIGTLRNDRGMFVHETILSLLATWRQQGRNPYEELTRVVHDNEMISREQTVPVVETSG
Blast result :
Comments
ISNpe8 is 98% aa similar to ISH10B.
IS_pep_1 is Natpe_0840, Natpe_1577, Natpe_3670, Natpe_3926, Natpe_4002, Natpe_4077, Natpe_4116, Natpe_4119, and Natpe_4124.
IS_pep_1 is Natpe_0840, Natpe_1577, Natpe_3670, Natpe_3926, Natpe_4002, Natpe_4077, Natpe_4116, Natpe_4119, and Natpe_4124.
References
1] Pfeiffer, F. (2013) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank