ISfinder Mirror Site

ISNpe3

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003373	ND	Natrinema pellirubrum	Natrinema pellirubrum DSM 15624 plasmid pNATPE01

DNA section

IS Length : 1055 bp

Ends

IR Length : 11/15

IRL : TAGTAACCGTTAGAACTTTTTACCCGTAGACTGTTGCAATAGGTAGTGGA
IRR : TAGTAGACACTAGAAGTAATTGCTCACTTTAGGAACAGAGAGTTTGTCGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTTCAATAGC	TA	TCTACGACGA	2

DNA sequence

TAGTAACCGTTAGAACTTTTTACCCGTAGACTGTTGCAATAGGTAGTGGATCACCTTGACGAAACCTCCGTCGAAGAGCTGCAAGATGCTCTCGACAAGG
TGGAGAGAAAGAAGCCGACACAGCGGCTACTGGCGGCGATTGCGTACAAAAATGGTGTGACGCAGACCGAATTAGCCGAGTGGTACGACGTTCAGCGGCG
GACGATCTATAGTTGGCTCAAGCGACTCGACACCGACGAGTCGCTTGAGCAGGCTGTTTCTGATGATAAGCGAACTGGGAGAAAGCGAAAGCTCTCAGAA
ACACAGCAAGAGGAATTTGAAGAAACTGTACACAAGTCTCCTGAAGAAGTTGGGATCGACGCGCCTGCGTGGACGCCGGCGCTCGCCCAAGAATATCTCG
AAGAAACCTACGGAGTCGAGTACTCAATCCCGAGTTGTCGGCGGTTGCTGAAAGAGGCGGGATTGAGTTACCAGAAACCGCGCCGTTCAGCCGCCGAAGC
CGATGAAGACGAGCAAGAAGAGTTCCGCAACGAGCTCAAAAAAAGCGGCGGGAGATGGACGCCACCGTAGTCTGTATCGATCAAACCAAGAAATCCGTGC
AAGTCGAGCCGCGTGCCGCGTGGTTTCCGCGTGGCACGCGGCCCTCTGTCGAACTCTCTGGCCAACGCGACTGGACGTGTCTGTTGGGCGCGATCACCGA
GGACGGTGATCGCTTCTTCTCCCGATTTGAGGAGTACGTCACCGCCGACCACGCAAAACATTTCATTTTGGCATTATGCGAAGAGTTCGAAGATGATTTG
ATCGTGGTGCTTGATGGAGCGCCGTATTTCCAGGCGTCGGCCGTCACGGACCTAGCGGCCCGTGACGACCTCGCCTTCGTGACGTTACCCTCGTATTCAC
CGGAGTTGAATCCGGTCGAAGAATGCTGGAGACAGCTGCAAAAGGCTCTTAGCAACCGTTTCTTTGACTCGCTCAACGAGCTAACAACAGCGATCGACAC
CGCTCTCGACAAACTCTCTGTTCCTAAAGTGAGCAATTACTTCTAGTGTCTACTA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
525 bp	174 aa	46	570	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDHLDETSVEELQDALDKVERKKPTQRLLAAIAYKNGVTQTELAEWYDVQRRTIYSWLKRLDTDESLEQAVSDDKRTGRKRKLSETQQEEFEETVHKSPE
EVGIDAPAWTPALAQEYLEETYGVEYSIPSCRRLLKEAGLSYQKPRRSAAEADEDEQEEFRNELKKSGGRWTPP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
540 bp	179 aa	507	1046	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RRARRVPQRAQKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRPSVELSGQRDWTCLLGAITEDGDRFFSRFEEYVTADHAKHFILALCEEFEDDLIV
VLDGAPYFQASAVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQKALSNRFFDSLNELTTAIDTALDKLSVPKVSNYF

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1001 bp	333 aa	46	1046	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDHLDETSVEELQDALDKVERKKPTQRLLAAIAYKNGVTQTELAEWYDVQRRTIYSWLKRLDTDESLEQAVSDDKRTGRKRKLSETQQEEFEETVHKSPE
EVGIDAPAWTPALAQEYLEETYGVEYSIPSCRRLLKEAGLSYQKPRRSAAEADEDEQEEFRNELKKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITEDGDRFFSRFEEYVTADHAKHFILALCEEFEDDLIVVLDGAPYFQASAVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQK
ALSNRFFDSLNELTTAIDTALDKLSVPKVSNYF

Blast result :

Comments

ISNpe3 is 96% aa similar to ISHvo16.
The third ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.

References

1] Pfeiffer, F. (2013) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T.(2012) Direct submission GenBank