ISNma8
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001934 | ND | Natrialba magadii | Natrialba magadii ATCC 43099 plasmid pNMAG02 |
DNA section
IS Length : 1055 bp
Ends
IR Length : 13/17
IRL : TAGTAACCGCTAGAACTTTCCGCCCACAGATTGTTGAAGCTACTAATGGA
IRR : TAGTAGACGTTAGAAATGATTGCTCACCTTTGGGAGCGAAAGTTGATCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAACTGCAAC | TA | GTTACACACT | 2 |
DNA sequence
TAGTAACCGCTAGAACTTTCCGCCCACAGATTGTTGAAGCTACTAATGGACCATCTCGACGAGATCTCCGTCGAAGAACTTCACGACGCACTCAACAACG
TCGAGGGGAGGAAGCCGACACAGCGACTGATAGCGGCAATTGAGTACAAAAACGGCGTTACGCAGACTGAACTAGCTGAGTGGCATAACACCGGCCGGCG
AACAATCTACAGTTGGCTCAAGCGACTCGATACTGACGAGTCGCTTGAGCAGGCTGTTACTGATGCTCACCGATCTGGGAGAAAACGAAAACTCTCAGAA
AAAGAGCAGCAAGAGTTCGAAGACACAGTCCACGAATCTCCCGAAGAAGTTGGGGTAGACGCGCCGGCGTGGACGCCGGCGCTCGTCCAGCAGTATCTTG
ACGAAACCTACGATGTCGAGTACTCAATCCCGAGCTGTCGGCGGTTGCTCAAAGAAGCGGGATTGAGCTATCAAAAACCACGACCTACAGCCGCCGAATC
AGATGCTGAGGACCAAGAAACGTTCCGCGAAGAACTCAAAAAAAGCGGCGGGAAATGGACGCCACGGTAGTCTGTATCGATCAAACCAAGAAATCCGTGC
AAGTTGAGCCGCGTGCCGCGTGGTTTCCGCGCGGCACACGGCCGTCCGTCGAACTGTCTGGGCAACGCGACTGGACGTGTCTGTTGGGCGCGATCACCGA
GAACGGTGATCGCTTTTTCACTCGATTCGAAGAGTACGTGACCGCCGCCCACGCAAAACATTTCATTCTTGCGTTATGCCAAGAGTTCGAAGATAATTTG
ATTGTCGTGCTAGATGGAGCGCCGTATTTTCAGGCGTCGGCCGTCACGGACCTGGCGGCCCGTGACGACCTCACCTTCGTGAGGTTACCGGCGTATTCTC
CCGAACTCAACCCAGTCGAGGAGTGCTGGAGACAGCTCCAGGCAGCCCTCAGCAACCGGTTCTTTGACTCGCTTCCTGAGCTCACAACAGCGATTGATAC
CGCTCTTGATCAACTTTCGCTCCCAAAGGTGAGCAATCATTTCTAACGTCTACTA
TCGAGGGGAGGAAGCCGACACAGCGACTGATAGCGGCAATTGAGTACAAAAACGGCGTTACGCAGACTGAACTAGCTGAGTGGCATAACACCGGCCGGCG
AACAATCTACAGTTGGCTCAAGCGACTCGATACTGACGAGTCGCTTGAGCAGGCTGTTACTGATGCTCACCGATCTGGGAGAAAACGAAAACTCTCAGAA
AAAGAGCAGCAAGAGTTCGAAGACACAGTCCACGAATCTCCCGAAGAAGTTGGGGTAGACGCGCCGGCGTGGACGCCGGCGCTCGTCCAGCAGTATCTTG
ACGAAACCTACGATGTCGAGTACTCAATCCCGAGCTGTCGGCGGTTGCTCAAAGAAGCGGGATTGAGCTATCAAAAACCACGACCTACAGCCGCCGAATC
AGATGCTGAGGACCAAGAAACGTTCCGCGAAGAACTCAAAAAAAGCGGCGGGAAATGGACGCCACGGTAGTCTGTATCGATCAAACCAAGAAATCCGTGC
AAGTTGAGCCGCGTGCCGCGTGGTTTCCGCGCGGCACACGGCCGTCCGTCGAACTGTCTGGGCAACGCGACTGGACGTGTCTGTTGGGCGCGATCACCGA
GAACGGTGATCGCTTTTTCACTCGATTCGAAGAGTACGTGACCGCCGCCCACGCAAAACATTTCATTCTTGCGTTATGCCAAGAGTTCGAAGATAATTTG
ATTGTCGTGCTAGATGGAGCGCCGTATTTTCAGGCGTCGGCCGTCACGGACCTGGCGGCCCGTGACGACCTCACCTTCGTGAGGTTACCGGCGTATTCTC
CCGAACTCAACCCAGTCGAGGAGTGCTGGAGACAGCTCCAGGCAGCCCTCAGCAACCGGTTCTTTGACTCGCTTCCTGAGCTCACAACAGCGATTGATAC
CGCTCTTGATCAACTTTCGCTCCCAAAGGTGAGCAATCATTTCTAACGTCTACTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 525 bp | 174 aa | 46 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
MDHLDEISVEELHDALNNVEGRKPTQRLIAAIEYKNGVTQTELAEWHNTGRRTIYSWLKRLDTDESLEQAVTDAHRSGRKRKLSEKEQQEFEDTVHESPE
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKSGGKWTPR
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKSGGKWTPR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 537 bp | 178 aa | 510 | 1046 | + | No |
Description : Second part of the transposase
ORF sequence :
GPRNVPRRTQKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRPSVELSGQRDWTCLLGAITENGDRFFTRFEEYVTAAHAKHFILALCQEFEDNLIVV
LDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQAALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
LDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQAALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1001 bp | 333 aa | 46 | 1046 | + | Yes |
Chemistry : DDE
ORF sequence :
MDHLDEISVEELHDALNNVEGRKPTQRLIAAIEYKNGVTQTELAEWHNTGRRTIYSWLKRLDTDESLEQAVTDAHRSGRKRKLSEKEQQEFEDTVHESPE
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITENGDRFFTRFEEYVTAAHAKHFILALCQEFEDNLIVVLDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQA
ALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITENGDRFFTRFEEYVTAAHAKHFILALCQEFEDNLIVVLDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQA
ALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
Blast result :
Comments
ISNma8 is 93% aa similar to ISHvo16 and ISNma7.
The ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
The integration site has a palindrome (TGcAAC/GTTaCA).
The ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
The integration site has a palindrome (TGcAAC/GTTaCA).
References
1] Pfeiffer, F. (2013) Direct submission
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. (2012) BMC Genomics 13: 165
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. (2012) BMC Genomics 13: 165