ISNma6
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001932 | ND | Natrialba magadii | Natrialba magadii ATCC 43099 |
DNA section
IS Length : 1055 bp
Ends
IR Length : 13/15
IRL : TAGTAATCGTTAGAACTTTCCGCTCAGAAATTGTTGTTATAATAAATGGA
IRR : TAGTAGACGTTAGAAATAATCACTCATTTTTGGAAGCGACAGTTGGTCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCCTTATAC | TA | GCAATCAAGA | 2 |
DNA sequence
TAGTAATCGTTAGAACTTTCCGCTCAGAAATTGTTGTTATAATAAATGGACCATCTCGACGAGATCTCCGTCGAAGAACTCCAAGATGCCCTCGACAATG
TCGAGGGAAACAAGCCGACACAACGGTTGTTAGCGGCGATTGCGTACAAGAGCGGTGTGACGCAAACCGAACTTGCAGAGTGGCACGACACCGGTCGAAG
AACGATCTACAGTTGGCTGATGCGACTTGATACGGACGAGCCGCTTGAGCAAGCCGTCTCTGATGCTCATCGAACCGGGAGAAAACGAAAGCTCTCAAAA
TCACAGCAAGAAGAGTTCGAACAAACCGTTCACGAACCGCCCGAAGAGGTTGGGATGGACGCGCCGGCGTGGACGCCGGCGCTCGTCCAAGAGTTTCTGG
AAGAAACCTACGGCGTCGAGTACTCCTATCCGAGTTGCCGGCGGCTCTTGAAAGAAGCTGGACTCAGTTATCAAAAACCTCGCCGCACAGCCGCCGAAGC
CGAAGAATCCGACCGAGAAGAATTCCACGACGAGATCAAAAAAAGCGATCGGAGATGGACGCCAAAATAGTCTGTATCGATCAAACCAAGAAATCGGTGC
AGGTCGAGCCGCGTGCCGCGTGGTTTCCTCGCGGCACGCGGCCGAGCGTCGAACTCTCTGGCCAACGCGACTGGACGTGTCTGCTCGGCGCGGTCACCGA
AGACGGCGAACGCTTTTTCTCACGATTCACCGAGTACGTCACCGCCGATCACGCAAAACATTTCATTCTCGTATTATGCAAAGAGTTCGAAGATGATCTG
ATCGTCGTGCTTGATGGAGCGCCGTATTTTCAGGCGTCGGCCGTCACGGACCTGGCGGCCCGTGACGACCTCACCTTCGTCACGTTCCCTGCGTATTCAC
CGGAACTCAATCCTGTCGAAGAGTGCTGGAGACAGCTCCAAGACGCTCTAAGCAACCGATTCTTTGACTCGTTAGACGAACTCACGACGGTAATCGATAC
CGCTCTTGACCAACTGTCGCTTCCAAAAATGAGTGATTATTTCTAACGTCTACTA
TCGAGGGAAACAAGCCGACACAACGGTTGTTAGCGGCGATTGCGTACAAGAGCGGTGTGACGCAAACCGAACTTGCAGAGTGGCACGACACCGGTCGAAG
AACGATCTACAGTTGGCTGATGCGACTTGATACGGACGAGCCGCTTGAGCAAGCCGTCTCTGATGCTCATCGAACCGGGAGAAAACGAAAGCTCTCAAAA
TCACAGCAAGAAGAGTTCGAACAAACCGTTCACGAACCGCCCGAAGAGGTTGGGATGGACGCGCCGGCGTGGACGCCGGCGCTCGTCCAAGAGTTTCTGG
AAGAAACCTACGGCGTCGAGTACTCCTATCCGAGTTGCCGGCGGCTCTTGAAAGAAGCTGGACTCAGTTATCAAAAACCTCGCCGCACAGCCGCCGAAGC
CGAAGAATCCGACCGAGAAGAATTCCACGACGAGATCAAAAAAAGCGATCGGAGATGGACGCCAAAATAGTCTGTATCGATCAAACCAAGAAATCGGTGC
AGGTCGAGCCGCGTGCCGCGTGGTTTCCTCGCGGCACGCGGCCGAGCGTCGAACTCTCTGGCCAACGCGACTGGACGTGTCTGCTCGGCGCGGTCACCGA
AGACGGCGAACGCTTTTTCTCACGATTCACCGAGTACGTCACCGCCGATCACGCAAAACATTTCATTCTCGTATTATGCAAAGAGTTCGAAGATGATCTG
ATCGTCGTGCTTGATGGAGCGCCGTATTTTCAGGCGTCGGCCGTCACGGACCTGGCGGCCCGTGACGACCTCACCTTCGTCACGTTCCCTGCGTATTCAC
CGGAACTCAATCCTGTCGAAGAGTGCTGGAGACAGCTCCAAGACGCTCTAAGCAACCGATTCTTTGACTCGTTAGACGAACTCACGACGGTAATCGATAC
CGCTCTTGACCAACTGTCGCTTCCAAAAATGAGTGATTATTTCTAACGTCTACTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 525 bp | 174 aa | 46 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
MDHLDEISVEELQDALDNVEGNKPTQRLLAAIAYKSGVTQTELAEWHDTGRRTIYSWLMRLDTDEPLEQAVSDAHRTGRKRKLSKSQQEEFEQTVHEPPE
EVGMDAPAWTPALVQEFLEETYGVEYSYPSCRRLLKEAGLSYQKPRRTAAEAEESDREEFHDEIKKSDRRWTPK
EVGMDAPAWTPALVQEFLEETYGVEYSYPSCRRLLKEAGLSYQKPRRTAAEAEESDREEFHDEIKKSDRRWTPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 783 bp | 260 aa | 264 | 1046 | + | No |
Description : Second part of the transposase
ORF sequence :
CSSNREKTKALKITARRVRTNRSRTARRGWDGRAGVDAGARPRVSGRNLRRRVLLSELPAALERSWTQLSKTSPHSRRSRRIRPRRIPRRDQKKRSEMDA
KIVCIDQTKKSVQVEPRAAWFPRGTRPSVELSGQRDWTCLLGAVTEDGERFFSRFTEYVTADHAKHFILVLCKEFEDDLIVVLDGAPYFQASAVTDLAAR
DDLTFVTFPAYSPELNPVEECWRQLQDALSNRFFDSLDELTTVIDTALDQLSLPKMSDYF
KIVCIDQTKKSVQVEPRAAWFPRGTRPSVELSGQRDWTCLLGAVTEDGERFFSRFTEYVTADHAKHFILVLCKEFEDDLIVVLDGAPYFQASAVTDLAAR
DDLTFVTFPAYSPELNPVEECWRQLQDALSNRFFDSLDELTTVIDTALDQLSLPKMSDYF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1001 bp | 333 aa | 46 | 1046 | + | Yes |
Chemistry : DDE
ORF sequence :
MDHLDEISVEELQDALDNVEGNKPTQRLLAAIAYKSGVTQTELAEWHDTGRRTIYSWLMRLDTDEPLEQAVSDAHRTGRKRKLSKSQQEEFEQTVHEPPE
EVGMDAPAWTPALVQEFLEETYGVEYSYPSCRRLLKEAGLSYQKPRRTAAEAEESDREEFHDEIKKKRSEMDAKIVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAVTEDGERFFSRFTEYVTADHAKHFILVLCKEFEDDLIVVLDGAPYFQASAVTDLAARDDLTFVTFPAYSPELNPVEECWRQLQD
ALSNRFFDSLDELTTVIDTALDQLSLPKMSDYF
EVGMDAPAWTPALVQEFLEETYGVEYSYPSCRRLLKEAGLSYQKPRRTAAEAEESDREEFHDEIKKKRSEMDAKIVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAVTEDGERFFSRFTEYVTADHAKHFILVLCKEFEDDLIVVLDGAPYFQASAVTDLAARDDLTFVTFPAYSPELNPVEECWRQLQD
ALSNRFFDSLDELTTVIDTALDQLSLPKMSDYF
Blast result :
Comments
ISNma6 is 92% aa similar to ISNma7 and ISHvo16.
The ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
The ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
References
1] Pfeiffer, F. (2013) Direct submission
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165