ISGxy1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP012160 | ND | Gluconacetobacter xylinus | Gluconobacter oxydans 621H Gluconacetobacter xylinus NBRC 3288 plasmid pGXY010 |
DNA section
IS Length : 1313 bp
Ends
IR Length : 21/26
IRL : AGAGGTGGTCCCCATTTTTCGGACAGGGCAATAAGCTATAATCCGACCTG
IRR : AGAGGTGGACCCGTCTGTTCGGACAGTTTTTGGGTTTGAATAAGCTATAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTATCCAGC | AGGTTCCGCT | 0 | |
| ATTCCCGTCA | TAA | CGCCATCCCG | 3 |
| CCTTCCGCAT | GTTTTCACAA | 0 | |
| CCCATCGCCC | AAGCACGCCT | 0 |
DNA sequence
AGAGGTGGTCCCCATTTTTCGGACAGGGCAATAAGCTATAATCCGACCTGAGAGAGAACGGGTTTTATGGGCAAGGTTACGCGCAAGAGGTATGCGGCGG
AGTTCAAATCACGGGTTGCCCTGGAAGCGATCCGTGGGGAACTGACGCTGGCGGAACTGGCTTCGAAACATGGGGTGCATCAGACGATGATCGCCCAGTG
GAAACGGCAGGCGATCGAGGGGATGGCGGCCACCTTTTCCGGGAAAGCGACGTCTGAGCCCCCGGTCAGCCCTGCTGATGTGGAGAAACTGCACGCGAAG
ATAGGCGAGCTTCTCGTGGAACGGGATTTTTTACGCGATGCCTCTGCTCGGTTGGGCGTGATCAGAGGCGGCAGATGATTGACCCGAAGCGAGCGCGTCT
GCCGATAATCCGGCAATGCACACTGCTGCGACTCAACCGGTCGGGCGTCTACTATCGGTCTGTGCCACAGAGTGAGGCCAATCTGGAGTTGATGCGGCTG
ATCGACGCCCAGTTCCTGGAAAGGCCGTATTATGGCTCGCGGCAGATGACATGGCATCTGCGCCGCCTGGGACATGAAGTGGGGCGCAAACGCGTCCGCC
GGCTCATGGCGATCATGGGCCTGCGGGCGATCTACCAGAAGCCACGCACGAGTGTGCCCCATCCGGAGCATCGGAAATATCCATATCTGCTGCGGGATCT
GGTCATTGATCGACCTAACCAGGTCTGGTGTTCCGATATCACATATATTCCCATGAAATGGGGATTTCTCTATCTCGTGGCGATCATGGACTGGTTCACG
CGCAAGGTGCTGTCCTGGCGTCTGTCGAACACGATGGACGCGGAGTTCTGTATTGAGGCACTTCGGGATGCGCTGATGCGTTACGGCACCCCGGAGATTT
TCAACACGGACCAGGGCTCACAATTCACGACGCCCCGTTTTACCGGTATCCTGGAAGAACGTCAGATCCGCATTAGCATGGACGGGCGTGGGCGCTGGCT
GGACAACGTGTTCATCGAGCGTCTGTGGCGGTCGTTGAAATACGAATGTGTCTACCTGCACGCATTTGAGACCGGATCAGAACTTCGAGTGGGGCTCACG
AAATGGATCACCCATTATAACGGGCAACGTCCCCATACGGCCCTGGCTGGACGAACGCCTGATGAGGCGTATCATGGCGCGCCGACGCCATCTGGTCCGG
CGTTAACCCCGGACCAGATGGCCAACAGCAACGCCGTCAGAATGGCGGCATAACAACAACCGGGTATAGCTTATTCAAACCCAAAAACTGTCCGAACAGA
CGGGTCCACCTCT
AGTTCAAATCACGGGTTGCCCTGGAAGCGATCCGTGGGGAACTGACGCTGGCGGAACTGGCTTCGAAACATGGGGTGCATCAGACGATGATCGCCCAGTG
GAAACGGCAGGCGATCGAGGGGATGGCGGCCACCTTTTCCGGGAAAGCGACGTCTGAGCCCCCGGTCAGCCCTGCTGATGTGGAGAAACTGCACGCGAAG
ATAGGCGAGCTTCTCGTGGAACGGGATTTTTTACGCGATGCCTCTGCTCGGTTGGGCGTGATCAGAGGCGGCAGATGATTGACCCGAAGCGAGCGCGTCT
GCCGATAATCCGGCAATGCACACTGCTGCGACTCAACCGGTCGGGCGTCTACTATCGGTCTGTGCCACAGAGTGAGGCCAATCTGGAGTTGATGCGGCTG
ATCGACGCCCAGTTCCTGGAAAGGCCGTATTATGGCTCGCGGCAGATGACATGGCATCTGCGCCGCCTGGGACATGAAGTGGGGCGCAAACGCGTCCGCC
GGCTCATGGCGATCATGGGCCTGCGGGCGATCTACCAGAAGCCACGCACGAGTGTGCCCCATCCGGAGCATCGGAAATATCCATATCTGCTGCGGGATCT
GGTCATTGATCGACCTAACCAGGTCTGGTGTTCCGATATCACATATATTCCCATGAAATGGGGATTTCTCTATCTCGTGGCGATCATGGACTGGTTCACG
CGCAAGGTGCTGTCCTGGCGTCTGTCGAACACGATGGACGCGGAGTTCTGTATTGAGGCACTTCGGGATGCGCTGATGCGTTACGGCACCCCGGAGATTT
TCAACACGGACCAGGGCTCACAATTCACGACGCCCCGTTTTACCGGTATCCTGGAAGAACGTCAGATCCGCATTAGCATGGACGGGCGTGGGCGCTGGCT
GGACAACGTGTTCATCGAGCGTCTGTGGCGGTCGTTGAAATACGAATGTGTCTACCTGCACGCATTTGAGACCGGATCAGAACTTCGAGTGGGGCTCACG
AAATGGATCACCCATTATAACGGGCAACGTCCCCATACGGCCCTGGCTGGACGAACGCCTGATGAGGCGTATCATGGCGCGCCGACGCCATCTGGTCCGG
CGTTAACCCCGGACCAGATGGCCAACAGCAACGCCGTCAGAATGGCGGCATAACAACAACCGGGTATAGCTTATTCAAACCCAAAAACTGTCCGAACAGA
CGGGTCCACCTCT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 312 bp | 103 aa | 67 | 378 | + | No |
Description : First part of the transposase
ORF sequence :
MGKVTRKRYAAEFKSRVALEAIRGELTLAELASKHGVHQTMIAQWKRQAIEGMAATFSGKATSEPPVSPADVEKLHAKIGELLVERDFLRDASARLGVIR
GGR
GGR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 875 bp | 324 aa | 379 | 1253 | + | No |
Description : Second part of the transposase
ORF sequence :
CGETAREDRRASRGTGFFTRCLCSVGRDQRRQMIDPKRARLPIIRQCTLLRLNRSGVYYRSVPQSEANLELMRLIDAQFLERPYYGSRQMTWHLRRLGHE
VGRKRVRRLMAIMGLRAIYQKPRTSVPHPEHRKYPYLLRDLVIDRPNQVWCSDITYIPMKWGFLYLVAIMDWFTRKVLSWRLSNTMDAEFCIEALRDALM
RYGTPEIFNTDQGSQFTTPRFTGILEERQIRISMDGRGRWLDNVFIERLWRSLKYECVYLHAFETGSELRVGLTKWITHYNGQRPHTALAGRTPDEAYHG
APTPSGPALTPDQMANSNAVRMAA
VGRKRVRRLMAIMGLRAIYQKPRTSVPHPEHRKYPYLLRDLVIDRPNQVWCSDITYIPMKWGFLYLVAIMDWFTRKVLSWRLSNTMDAEFCIEALRDALM
RYGTPEIFNTDQGSQFTTPRFTGILEERQIRISMDGRGRWLDNVFIERLWRSLKYECVYLHAFETGSELRVGLTKWITHYNGQRPHTALAGRTPDEAYHG
APTPSGPALTPDQMANSNAVRMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 395 aa | 67 | 1256 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKVTRKRYAAEFKSRVALEAIRGELTLAELASKHGVHQTMIAQWKRQAIEGMAATFSGKATSEPPVSPADVEKLHAKIGELLVERDFFTRCLCSVGRDQ
RRQMIDPKRARLPIIRQCTLLRLNRSGVYYRSVPQSEANLELMRLIDAQFLERPYYGSRQMTWHLRRLGHEVGRKRVRRLMAIMGLRAIYQKPRTSVPHP
EHRKYPYLLRDLVIDRPNQVWCSDITYIPMKWGFLYLVAIMDWFTRKVLSWRLSNTMDAEFCIEALRDALMRYGTPEIFNTDQGSQFTTPRFTGILEERQ
IRISMDGRGRWLDNVFIERLWRSLKYECVYLHAFETGSELRVGLTKWITHYNGQRPHTALAGRTPDEAYHGAPTPSGPALTPDQMANSNAVRMAA
RRQMIDPKRARLPIIRQCTLLRLNRSGVYYRSVPQSEANLELMRLIDAQFLERPYYGSRQMTWHLRRLGHEVGRKRVRRLMAIMGLRAIYQKPRTSVPHP
EHRKYPYLLRDLVIDRPNQVWCSDITYIPMKWGFLYLVAIMDWFTRKVLSWRLSNTMDAEFCIEALRDALMRYGTPEIFNTDQGSQFTTPRFTGILEERQ
IRISMDGRGRWLDNVFIERLWRSLKYECVYLHAFETGSELRVGLTKWITHYNGQRPHTALAGRTPDEAYHGAPTPSGPALTPDQMANSNAVRMAA
Blast result :
Comments
ISGxy1 is 79% aa similar to ISMch1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This IS has atypic IRs: it begins with 'AG' instead of 'TG'.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This IS has atypic IRs: it begins with 'AG' instead of 'TG'.
References
1] Mayya A. Petrova (2013) Direct submission to ISfinder
2] Ogino,H., Azuma,Y., Hosoyama,A., Nakazawa,H., Matsutani,M., Hasegawa,A., Otsuyama,K., Matsushita,K., Fujita,N. and Shirai,M. (2011) J. Bacteriol. 193 (24), 6997-6998
2] Ogino,H., Azuma,Y., Hosoyama,A., Nakazawa,H., Matsutani,M., Hasegawa,A., Otsuyama,K., Matsushita,K., Fujita,N. and Shirai,M. (2011) J. Bacteriol. 193 (24), 6997-6998