ISAfe17
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002985 | ND | Acidithiobacillus ferrivorans | Acidithiobacillus ferrivorans SS3 |
DNA section
IS Length : 1334 bp
Ends
IR Length : 20/26
IRL : TATAGTGGACCCCAGATTTGAGACAATAGTTTAAGCTTGTTGTCTGGTCA
IRR : TATGGTGGACCCATGAGTCAAGACAAAAAGAGCGAGAGTTTAAGCTAGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAAGAGACG | GGT | GCCTTAGCCT | 3 |
| GCCTGATGAG | TGC | CGCCGTATTT | 3 |
| CTACGATGAA | ATC | CGCGGATTTG | 3 |
| TTCGAATAAG | CTGCAGAGCG | 0 | |
| TGCGGCACAC | ATGGACAATG | 0 |
DNA sequence
TATAGTGGACCCCAGATTTGAGACAATAGTTTAAGCTTGTTGTCTGGTCAGGAGGAGACGATGAGCGCAGCAAAGCGGAAAGTGTATGGAGCCGCCTTTA
AGGGCAAGGTGGGGCTGGAGGCGATCCGGGGCCTGAAAACCATCAACGAGATTGCGCAGGAGCATGGAGTGCATCCGGCGCAGGTCGGGCAATGGAAGAA
GGCTATTCTGGAGCAGGTGGGTACTTTATTTGAGGGCAAGCGTGGCCCACAGCCCATTGATGCGCACAGCGACCCGGAGCATCTCTACAGTGAAATCGGG
CGGTTGAAAGTCGAACTGGATTGGCTGAAAAAAAAGTCCGGAGTGTGACGGTAGAGACGCGGCGGACGTGGATCGCACCGAATCCGTCGATCTCTATTCA
ACGGCAGTGTCACCTGGCTGGCGTGGCCCGAGCCACGGTTTACGCGCAGCGACCCGAAAAGCCATTGGACGCAGAGGATAAAGTGCTCATGGATTTACTG
GATGCCCAATACACAACAACGCCGTTTTATGGGAGTCGCCGGATGACCGTATATCTCCAAAATCTGGGGTTTAGGGTCAATCGCAAACGGGTGCAGCGAT
TGATGCGCACCTTGGGTTTGGCGGGCATGGCTCCAGGGCCGCACACCAGCCGACCCCATCCAGAGCATAAGATTTATCCCTACCTGCTGCGGGGGCTGAC
TATTGATCGCCCCGGTCAGGTCTGGAGTACGGATATCACGTATATCCGGCTCCGCCATGGATTTGTCTATCTGGTGGCTATTATGGACTGGTACAGCCGT
AAGGTGTTGAGCTGGAGGATCAGTAATACCATGGAGGCAGAGTTCTGTGTGGCCTGTCTGGAAGAGGCGCTACAACGCTATGGTGCGCCGGAGATCTTCA
ATACGGACCAAGGCAGCCAATTCACCGCTGAGGCGTTTACAGGCTTACTTCAGGCGCATGGTGTACAGATCAGCATGGACGGTCGTGGTCGCGCCCTGGA
TAACATCTTCGTGGAGCGGCTTTGGCGCAGTGTGAAGTACGAGGATATTTACCTGCGGGGCTATGGCCATGTACCGGAACTGATGGTTGGGCTGACTGCC
TACTTTGAGTTTTACAATAGTCGCCGCCCGCATCAGTCTCTCGGCTATCTGACACCAGATCAGGTATACCTGACAGGGCAAGGGGGCGGCGCCTGCATTG
TGGACCGCTATCCGAAGGTTGCAGCATAACCATTACTCAGGGACAATTTGGGGCAGCGCCATTCTGCTGCAATGTTAGAAACCAGACTAGCTTAAACTCT
CGCTCTTTTTGTCTTGACTCATGGGTCCACCATA
AGGGCAAGGTGGGGCTGGAGGCGATCCGGGGCCTGAAAACCATCAACGAGATTGCGCAGGAGCATGGAGTGCATCCGGCGCAGGTCGGGCAATGGAAGAA
GGCTATTCTGGAGCAGGTGGGTACTTTATTTGAGGGCAAGCGTGGCCCACAGCCCATTGATGCGCACAGCGACCCGGAGCATCTCTACAGTGAAATCGGG
CGGTTGAAAGTCGAACTGGATTGGCTGAAAAAAAAGTCCGGAGTGTGACGGTAGAGACGCGGCGGACGTGGATCGCACCGAATCCGTCGATCTCTATTCA
ACGGCAGTGTCACCTGGCTGGCGTGGCCCGAGCCACGGTTTACGCGCAGCGACCCGAAAAGCCATTGGACGCAGAGGATAAAGTGCTCATGGATTTACTG
GATGCCCAATACACAACAACGCCGTTTTATGGGAGTCGCCGGATGACCGTATATCTCCAAAATCTGGGGTTTAGGGTCAATCGCAAACGGGTGCAGCGAT
TGATGCGCACCTTGGGTTTGGCGGGCATGGCTCCAGGGCCGCACACCAGCCGACCCCATCCAGAGCATAAGATTTATCCCTACCTGCTGCGGGGGCTGAC
TATTGATCGCCCCGGTCAGGTCTGGAGTACGGATATCACGTATATCCGGCTCCGCCATGGATTTGTCTATCTGGTGGCTATTATGGACTGGTACAGCCGT
AAGGTGTTGAGCTGGAGGATCAGTAATACCATGGAGGCAGAGTTCTGTGTGGCCTGTCTGGAAGAGGCGCTACAACGCTATGGTGCGCCGGAGATCTTCA
ATACGGACCAAGGCAGCCAATTCACCGCTGAGGCGTTTACAGGCTTACTTCAGGCGCATGGTGTACAGATCAGCATGGACGGTCGTGGTCGCGCCCTGGA
TAACATCTTCGTGGAGCGGCTTTGGCGCAGTGTGAAGTACGAGGATATTTACCTGCGGGGCTATGGCCATGTACCGGAACTGATGGTTGGGCTGACTGCC
TACTTTGAGTTTTACAATAGTCGCCGCCCGCATCAGTCTCTCGGCTATCTGACACCAGATCAGGTATACCTGACAGGGCAAGGGGGCGGCGCCTGCATTG
TGGACCGCTATCCGAAGGTTGCAGCATAACCATTACTCAGGGACAATTTGGGGCAGCGCCATTCTGCTGCAATGTTAGAAACCAGACTAGCTTAAACTCT
CGCTCTTTTTGTCTTGACTCATGGGTCCACCATA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 288 bp | 95 aa | 61 | 348 | + | No |
Description : First part of the transposase
ORF sequence :
MSAAKRKVYGAAFKGKVGLEAIRGLKTINEIAQEHGVHPAQVGQWKKAILEQVGTLFEGKRGPQPIDAHSDPEHLYSEIGRLKVELDWLKKKSGV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 936 bp | 311 aa | 294 | 1229 | + | No |
Description : Second part of the transposase
ORF sequence :
NRAVESRTGLAEKKVRSVTVETRRTWIAPNPSISIQRQCHLAGVARATVYAQRPEKPLDAEDKVLMDLLDAQYTTTPFYGSRRMTVYLQNLGFRVNRKRV
QRLMRTLGLAGMAPGPHTSRPHPEHKIYPYLLRGLTIDRPGQVWSTDITYIRLRHGFVYLVAIMDWYSRKVLSWRISNTMEAEFCVACLEEALQRYGAPE
IFNTDQGSQFTAEAFTGLLQAHGVQISMDGRGRALDNIFVERLWRSVKYEDIYLRGYGHVPELMVGLTAYFEFYNSRRPHQSLGYLTPDQVYLTGQGGGA
CIVDRYPKVAA
QRLMRTLGLAGMAPGPHTSRPHPEHKIYPYLLRGLTIDRPGQVWSTDITYIRLRHGFVYLVAIMDWYSRKVLSWRISNTMEAEFCVACLEEALQRYGAPE
IFNTDQGSQFTAEAFTGLLQAHGVQISMDGRGRALDNIFVERLWRSVKYEDIYLRGYGHVPELMVGLTAYFEFYNSRRPHQSLGYLTPDQVYLTGQGGGA
CIVDRYPKVAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1169 bp | 389 aa | 61 | 1229 | + | Yes |
Chemistry : DDE
ORF sequence :
MSAAKRKVYGAAFKGKVGLEAIRGLKTINEIAQEHGVHPAQVGQWKKAILEQVGTLFEGKRGPQPIDAHSDPEHLYSEIGRLKVELDWLKKKVRSVTVET
RRTWIAPNPSISIQRQCHLAGVARATVYAQRPEKPLDAEDKVLMDLLDAQYTTTPFYGSRRMTVYLQNLGFRVNRKRVQRLMRTLGLAGMAPGPHTSRPH
PEHKIYPYLLRGLTIDRPGQVWSTDITYIRLRHGFVYLVAIMDWYSRKVLSWRISNTMEAEFCVACLEEALQRYGAPEIFNTDQGSQFTAEAFTGLLQAH
GVQISMDGRGRALDNIFVERLWRSVKYEDIYLRGYGHVPELMVGLTAYFEFYNSRRPHQSLGYLTPDQVYLTGQGGGACIVDRYPKVAA
RRTWIAPNPSISIQRQCHLAGVARATVYAQRPEKPLDAEDKVLMDLLDAQYTTTPFYGSRRMTVYLQNLGFRVNRKRVQRLMRTLGLAGMAPGPHTSRPH
PEHKIYPYLLRGLTIDRPGQVWSTDITYIRLRHGFVYLVAIMDWYSRKVLSWRISNTMEAEFCVACLEEALQRYGAPEIFNTDQGSQFTAEAFTGLLQAH
GVQISMDGRGRALDNIFVERLWRSVKYEDIYLRGYGHVPELMVGLTAYFEFYNSRRPHQSLGYLTPDQVYLTGQGGGACIVDRYPKVAA
Blast result :
Comments
ISAfe17 is 67% aa similar to ISPpy1.
There are 5 copies of ISAfe17 in the chromosome of Acidithiobacillus ferrivorans SS3.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
There are 5 copies of ISAfe17 in the chromosome of Acidithiobacillus ferrivorans SS3.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Mayya A. Petrova (2013) Direct submission to ISfinder
2] Liljeqvist,M., Valdes,J., Holmes,D.S. and Dopson,M. (2011) J. Bacteriol. 193 (16), 4304-4305
2] Liljeqvist,M., Valdes,J., Holmes,D.S. and Dopson,M. (2011) J. Bacteriol. 193 (16), 4304-4305