ISEnfa4
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JQ911741 | ND | Enterococcus faecealis | Enterococcus faecealis plasmid pW9-2 |
DNA section
IS Length : 1324 bp
Ends
IR Length : 17/26
IRL : GATAAAGTCCGTATAATTGTGTAAAAGTAAAAAGGCCATATAACAGTCCT
IRR : AGTCAAGTCCAGACTCCTGTGTAAAATGCTATACAATGTTTTTACCATTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATTTGGATA | TAGAATCA | GTAAATGAAA | 8 |
DNA sequence
GATAAAGTCCGTATAATTGTGTAAAAGTAAAAAGGCCATATAACAGTCCTTTTACGGTACAATGTTTTTAACGACAAAAACATACCCAGGAGGACTTTTA
CATGACCCAAGTACATTTTACACTGAAAAGCGAAGAGATTCAAAGCATTATTGAATATTCTGTAAAGGATGACGTTTCTAAAAATATTTTAACAACGGTA
TTTAATCAACTAATGGAAAATCAACGAACAGAATATATTCAAGCAAAAGAATATGAACGAACAGAAAACCGACAAAGTCAACGAAATGGCTATTATGAGC
GCAGCTTTACGACACGTGTAGGCACGCTAGAATTAAAAGTACCCAGAACACGTGATGGTGAATTTTCACCGACGGTGTTTGAGCGTTATCAGCGAAATGA
AAAGGCACTGCTCGCTTCAATGCTTGAGATGTATGTTTCAGGCGTTTCGACACGTAAAGTTTCAAAGATTGTTGAAGAGCTATGTGGAAAATCGGTATCG
AAATCTTTTGTTTCTAGCCTGACTGAGCAGTTAGACCCGATGGTCAACGAATGGCAGAACCGTTCACTCTCAGGTACGAGTTATCCTTATCTGATGACTG
ATGTTCTCTACATAAAAGTCCGTGAGGACCATCGAGTGCTTTCTAAAAGCTGCCATATTGCGATCGGGATAACAGAAGGTGGTGATCGTGAAATCATTGG
CTTCATGATCCAAAATGAAGAAAGTGATGACACATGGTCCATCTTCTTTGAATACTTAAAAGAACGCGGTCTACAGGGGACAGAACTCATCATTTCTGAT
GCCCATAAAGGCCTAGTGTCTGCAATTCGTAAGTCATTTACCAACGCAAGTTGGCAGAGATGCCAGGTCCATTTTTTAAGAAACATCTTCAGTTCCATTC
CAAAAAAGAATTCAAAACCGTTTAGAGAAGCAGTAAAAGCGATCTTTAAGTTTACGGATATTGAACTCGCTCGAACAGCTAAGAATGCCTTAGTCGGTGA
ATATATCGACCAGTCTAAATATTCAAAAGCTTGCGCATCATTGGATGATGGATTCGAAGACGCCTTTCAATATACCGTACAAGGAAATTCCCACAATCGA
CTAAAGAGTACCAATCTAATTGAACGACTGAATCAAGAAGTACGCAGAAGAGAAAAGATTATTCGCATCTTCCCCAATCAAACATCAGCCAATCGCTTAA
TTGGAGCCGTTCTTATGGACCTACATGATGAATGGATTTATTCTTCAAGAAAATACATCAATTTTGATAAGTAGAAATGGTAAAAACATTGTATAGCATT
TTACACAGGAGTCTGGACTTGACT
CATGACCCAAGTACATTTTACACTGAAAAGCGAAGAGATTCAAAGCATTATTGAATATTCTGTAAAGGATGACGTTTCTAAAAATATTTTAACAACGGTA
TTTAATCAACTAATGGAAAATCAACGAACAGAATATATTCAAGCAAAAGAATATGAACGAACAGAAAACCGACAAAGTCAACGAAATGGCTATTATGAGC
GCAGCTTTACGACACGTGTAGGCACGCTAGAATTAAAAGTACCCAGAACACGTGATGGTGAATTTTCACCGACGGTGTTTGAGCGTTATCAGCGAAATGA
AAAGGCACTGCTCGCTTCAATGCTTGAGATGTATGTTTCAGGCGTTTCGACACGTAAAGTTTCAAAGATTGTTGAAGAGCTATGTGGAAAATCGGTATCG
AAATCTTTTGTTTCTAGCCTGACTGAGCAGTTAGACCCGATGGTCAACGAATGGCAGAACCGTTCACTCTCAGGTACGAGTTATCCTTATCTGATGACTG
ATGTTCTCTACATAAAAGTCCGTGAGGACCATCGAGTGCTTTCTAAAAGCTGCCATATTGCGATCGGGATAACAGAAGGTGGTGATCGTGAAATCATTGG
CTTCATGATCCAAAATGAAGAAAGTGATGACACATGGTCCATCTTCTTTGAATACTTAAAAGAACGCGGTCTACAGGGGACAGAACTCATCATTTCTGAT
GCCCATAAAGGCCTAGTGTCTGCAATTCGTAAGTCATTTACCAACGCAAGTTGGCAGAGATGCCAGGTCCATTTTTTAAGAAACATCTTCAGTTCCATTC
CAAAAAAGAATTCAAAACCGTTTAGAGAAGCAGTAAAAGCGATCTTTAAGTTTACGGATATTGAACTCGCTCGAACAGCTAAGAATGCCTTAGTCGGTGA
ATATATCGACCAGTCTAAATATTCAAAAGCTTGCGCATCATTGGATGATGGATTCGAAGACGCCTTTCAATATACCGTACAAGGAAATTCCCACAATCGA
CTAAAGAGTACCAATCTAATTGAACGACTGAATCAAGAAGTACGCAGAAGAGAAAAGATTATTCGCATCTTCCCCAATCAAACATCAGCCAATCGCTTAA
TTGGAGCCGTTCTTATGGACCTACATGATGAATGGATTTATTCTTCAAGAAAATACATCAATTTTGATAAGTAGAAATGGTAAAAACATTGTATAGCATT
TTACACAGGAGTCTGGACTTGACT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1173 bp | 390 aa | 102 | 1274 | + | No |
Chemistry : DDE
ORF sequence :
MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTEYIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGEFSPTVFERYQRNE
KALLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTSYPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIG
FMIQNEESDDTWSIFFEYLKERGLQGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFREAVKAIFKFTDIELARTAKNALVGE
YIDQSKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK
KALLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTSYPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIG
FMIQNEESDDTWSIFFEYLKERGLQGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFREAVKAIFKFTDIELARTAKNALVGE
YIDQSKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK
Blast result :
Comments
ISEnfa4 is 97% aa similar to IS1542.
ISEnfa4 is associated with the multiresistance gene cfr coding for an rRNA methyltransferase specifying resistance to five chemically unrelated antimicrobial classes including phenicols, lincosamides, oxazolidionones, pleuromutilins, and streptogramin A, in a composite transposon structure. Its name is Tn6195 in the conjugative plasmid pW9-2 from Enterococcus faecalis W9-2 derived from sewage at a pig farm.
ISEnfa4 is associated with the multiresistance gene cfr coding for an rRNA methyltransferase specifying resistance to five chemically unrelated antimicrobial classes including phenicols, lincosamides, oxazolidionones, pleuromutilins, and streptogramin A, in a composite transposon structure. Its name is Tn6195 in the conjugative plasmid pW9-2 from Enterococcus faecalis W9-2 derived from sewage at a pig farm.
References
1] Liu, Y., Wang, Y., Schwarz, S., Shen, Z., Zhang, Q., Wu, C., and Shen, J. (2012) Direct ISfinder submission.