ISHaha3
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HE717023 | ND | Halobacillus halophilus | Halobacillus halophilus DSM 2266 |
DNA section
IS Length : 1437 bp
Ends
IR Length : 22/29
IRL : TGTAATGACCCCCAAATGTTGGACACCTTATGAAACTTAGGAGGGACCCA
IRR : TGAACTGACCCCTTAAAATTGGACACATTCATTAAAATTATCACGCCGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGAAGTCGA | AGA | CTGAGGAGGC | 3 |
| GCCTCCTCAG | TCT | TCGACTTCCG | 3 |
| CCTCAGTCGT | TTC | ACTCCTTGCG | 3 |
| GCCTCCTCGA | GCAA | GCTCTGCGGG | 4 |
| TCCTCAGTCG | CTT | CACTCCCTGT | 3 |
| GCCTCCTCAG | TCTT | CGACTTCCGG | 4 |
| CGGAAGGCTT | TAGC | CTGAGGAGGC | 4 |
DNA sequence
TGTAATGACCCCCAAATGTTGGACACCTTATGAAACTTAGGAGGGACCCAACATATGTCTAAACTTACTTCTCAAGAAAAGTACCTGATCGTCCAACGTT
ATCTCACGGAAAATGTCAGTTTCCGTGACTTAGAAAAAGAGTTTGGAGTGGATAGTTCTTCCATCCGTTATTGGGTGAAATTATCCGAATATCATGGTAG
TGAAGCCTTCGACTTCCCCTATACAAACTATTCAGCTGCCTTTAAACTGAAAGTAATTCAACGTATAGAGGAAGAAGAATATTCGATTCGAGAAGCTTCC
GCTATGTTTCACATCCCCGATTTCTCCATGGTACGCCGATGGCGTAAGAAGTGGCTGGAAGGCGGAAAAGAAGCCCTTGAATCTACAAGGAAGGGGCCAA
CACAGATGACGTCTCATAAGAAGAAAGAAGATTCAATGGATTCTTATGAAGCCTTAAAGAAAGAGAATGAACGCTTACGGATGGAAAATGAATACTTAAA
AAAGTTAGAGACCTTAGCTCAAAAAAAGAAAGCATCTCGAAAGAAGAACAAGCGCAAGTAATCGATGAGTTAAGGTTTCATTTTCCAGTTAAACAGCTGA
CGGAAATAGCTGATATCCCTAGAAGTACCTATTACCATTGGAAAGAAAAGCAACTCGAGCCAAAAACGGACCGACCATTAAAGGAGCTTATCACCTCCAT
TTTTAAAGAACATAAAGGTCGCTACGGCTATCGCCGCATTGAAAATGAGCTTCGTAATCTAGGTCATGAAGTGAACCATAAAAAAGTCTATCGTCTTATG
AAAGAACTGGGGCTTCAGTCTACGGTGAAAATGAAGAAATATCGTTCTTATAAAGGGAAAGTTGGTCAAACGGCTCCAAACATCTTAAATCGGAATTTCA
AGGCGGATCAGCCGAATCAGAAATGGGTGACAGACATTACGGAGTTCAAGCTATTTGGCGAGAAGCTTTATCTTTCCCCTGTCTTAGATCTATTCAATGG
AGAAATCATCACGTACACAATTGGTTCCAGACCCACCTATTCCTTGGTGAACAACATGTTAGACCAAGCATTTGAACGCCTTCATCCATCGGATGAACTG
GTTATGCACTCCGACCAGGGATGGCATTACCAAATGGCTCCCTATCGAAAGAAATTGAAAGACCATCAAGTTACCCAAAGCATGTCCAGAAAAGGGAATT
GTCATGATAACGCCGTTATGGAGAACTTTTTCGGTATCATGAAATCGGAATTCCTTTATTTAAGAGAATTTGAAAGTGTGGCCCATTTCAAACAAGAATT
AGAGGAATATATCCATTATTATAATCATAAACGCATCAAATCGAAGCTCAGAATAAGCCCCATTTCCTATCGAGCCCGCTTTCAAGAATCGGCGTGATAA
TTTTAATGAATGTGTCCAATTTTAAGGGGTCAGTTCA
ATCTCACGGAAAATGTCAGTTTCCGTGACTTAGAAAAAGAGTTTGGAGTGGATAGTTCTTCCATCCGTTATTGGGTGAAATTATCCGAATATCATGGTAG
TGAAGCCTTCGACTTCCCCTATACAAACTATTCAGCTGCCTTTAAACTGAAAGTAATTCAACGTATAGAGGAAGAAGAATATTCGATTCGAGAAGCTTCC
GCTATGTTTCACATCCCCGATTTCTCCATGGTACGCCGATGGCGTAAGAAGTGGCTGGAAGGCGGAAAAGAAGCCCTTGAATCTACAAGGAAGGGGCCAA
CACAGATGACGTCTCATAAGAAGAAAGAAGATTCAATGGATTCTTATGAAGCCTTAAAGAAAGAGAATGAACGCTTACGGATGGAAAATGAATACTTAAA
AAAGTTAGAGACCTTAGCTCAAAAAAAGAAAGCATCTCGAAAGAAGAACAAGCGCAAGTAATCGATGAGTTAAGGTTTCATTTTCCAGTTAAACAGCTGA
CGGAAATAGCTGATATCCCTAGAAGTACCTATTACCATTGGAAAGAAAAGCAACTCGAGCCAAAAACGGACCGACCATTAAAGGAGCTTATCACCTCCAT
TTTTAAAGAACATAAAGGTCGCTACGGCTATCGCCGCATTGAAAATGAGCTTCGTAATCTAGGTCATGAAGTGAACCATAAAAAAGTCTATCGTCTTATG
AAAGAACTGGGGCTTCAGTCTACGGTGAAAATGAAGAAATATCGTTCTTATAAAGGGAAAGTTGGTCAAACGGCTCCAAACATCTTAAATCGGAATTTCA
AGGCGGATCAGCCGAATCAGAAATGGGTGACAGACATTACGGAGTTCAAGCTATTTGGCGAGAAGCTTTATCTTTCCCCTGTCTTAGATCTATTCAATGG
AGAAATCATCACGTACACAATTGGTTCCAGACCCACCTATTCCTTGGTGAACAACATGTTAGACCAAGCATTTGAACGCCTTCATCCATCGGATGAACTG
GTTATGCACTCCGACCAGGGATGGCATTACCAAATGGCTCCCTATCGAAAGAAATTGAAAGACCATCAAGTTACCCAAAGCATGTCCAGAAAAGGGAATT
GTCATGATAACGCCGTTATGGAGAACTTTTTCGGTATCATGAAATCGGAATTCCTTTATTTAAGAGAATTTGAAAGTGTGGCCCATTTCAAACAAGAATT
AGAGGAATATATCCATTATTATAATCATAAACGCATCAAATCGAAGCTCAGAATAAGCCCCATTTCCTATCGAGCCCGCTTTCAAGAATCGGCGTGATAA
TTTTAATGAATGTGTCCAATTTTAAGGGGTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 55 | 561 | + | No |
Description : First part of the transposase
ORF sequence :
MSKLTSQEKYLIVQRYLTENVSFRDLEKEFGVDSSSIRYWVKLSEYHGSEAFDFPYTNYSAAFKLKVIQRIEEEEYSIREASAMFHIPDFSMVRRWRKKW
LEGGKEALESTRKGPTQMTSHKKKEDSMDSYEALKKENERLRMENEYLKKLETLAQKKKASRKKNKRK
LEGGKEALESTRKGPTQMTSHKKKEDSMDSYEALKKENERLRMENEYLKKLETLAQKKKASRKKNKRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 492 | 1397 | + | No |
Description : Second part of the transposase
ORF sequence :
ILKKVRDLSSKKESISKEEQAQVIDELRFHFPVKQLTEIADIPRSTYYHWKEKQLEPKTDRPLKELITSIFKEHKGRYGYRRIENELRNLGHEVNHKKVY
RLMKELGLQSTVKMKKYRSYKGKVGQTAPNILNRNFKADQPNQKWVTDITEFKLFGEKLYLSPVLDLFNGEIITYTIGSRPTYSLVNNMLDQAFERLHPS
DELVMHSDQGWHYQMAPYRKKLKDHQVTQSMSRKGNCHDNAVMENFFGIMKSEFLYLREFESVAHFKQELEEYIHYYNHKRIKSKLRISPISYRARFQES
A
RLMKELGLQSTVKMKKYRSYKGKVGQTAPNILNRNFKADQPNQKWVTDITEFKLFGEKLYLSPVLDLFNGEIITYTIGSRPTYSLVNNMLDQAFERLHPS
DELVMHSDQGWHYQMAPYRKKLKDHQVTQSMSRKGNCHDNAVMENFFGIMKSEFLYLREFESVAHFKQELEEYIHYYNHKRIKSKLRISPISYRARFQES
A
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1343 bp | 447 aa | 55 | 1397 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKLTSQEKYLIVQRYLTENVSFRDLEKEFGVDSSSIRYWVKLSEYHGSEAFDFPYTNYSAAFKLKVIQRIEEEEYSIREASAMFHIPDFSMVRRWRKKW
LEGGKEALESTRKGPTQMTSHKKKEDSMDSYEALKKENERLRMENEYLKKVRDLSSKKESISKEEQAQVIDELRFHFPVKQLTEIADIPRSTYYHWKEKQ
LEPKTDRPLKELITSIFKEHKGRYGYRRIENELRNLGHEVNHKKVYRLMKELGLQSTVKMKKYRSYKGKVGQTAPNILNRNFKADQPNQKWVTDITEFKL
FGEKLYLSPVLDLFNGEIITYTIGSRPTYSLVNNMLDQAFERLHPSDELVMHSDQGWHYQMAPYRKKLKDHQVTQSMSRKGNCHDNAVMENFFGIMKSEF
LYLREFESVAHFKQELEEYIHYYNHKRIKSKLRISPISYRARFQESA
LEGGKEALESTRKGPTQMTSHKKKEDSMDSYEALKKENERLRMENEYLKKVRDLSSKKESISKEEQAQVIDELRFHFPVKQLTEIADIPRSTYYHWKEKQ
LEPKTDRPLKELITSIFKEHKGRYGYRRIENELRNLGHEVNHKKVYRLMKELGLQSTVKMKKYRSYKGKVGQTAPNILNRNFKADQPNQKWVTDITEFKL
FGEKLYLSPVLDLFNGEIITYTIGSRPTYSLVNNMLDQAFERLHPSDELVMHSDQGWHYQMAPYRKKLKDHQVTQSMSRKGNCHDNAVMENFFGIMKSEF
LYLREFESVAHFKQELEEYIHYYNHKRIKSKLRISPISYRARFQESA
Blast result :
Comments
ISHaha3 is 76% aa similar to ISBth10.
The third ORF is a ORFAB transposase reconstructed to represent a probable -1 ribosome slippage.
The third ORF is a ORFAB transposase reconstructed to represent a probable -1 ribosome slippage.
References