ISAba22
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001937 | ND | Acinetobacter baumannii | Acinetobacter baumannii WM99c Acinetobacter baumannii MDR-ZJ06 |
DNA section
IS Length : 1274 bp
Ends
IR Length : 26/36
IRL : TGATGTGTGCTGAAAAAACAGGAGGTTTTAACTTGGTAAACTAAACACAC
IRR : TGATATACGCTGAATTATCAGGAGACTTTCCCTTGGGAGATTCTAGGCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGGACATAC | TTA | TGACCTATTA | 3 |
DNA sequence
TGATGTGTGCTGAAAAAACAGGAGGTTTTAACTTGGTAAACTAAACACACTCATCAGGAGTTTACCATGAGCAAGAAACACAAGACTTACACCACAGAAT
TTAAAGCTGAAGCCATCAAATTAATTGAAGCCAATCAAGGCAATGTCTCGGAAACAGCCAGACAACTTAGCATTTCAATGCAAACTCTTTCAAATTGGAA
TACCAAAGCAAAGGCTGGAACTTTAGCAGGTACAAAACAGTATTCACCTGATCTAAACGCTCTACTCGAAGAAAATAAAAAACTCAAACAACAGCTCAAA
ATAGCTGAAATGGAACGTGAATTTTTAAAAAAGGCAGCAGCGTACTTTGCCAAAGAAAGTCAGTAAGGTACGCCTATATGAAACAAAAAAGATATTCTTT
TCCAATTACCTTAATGGCTCGATTACTTCATGTTTCAGTTTCATGTTTTTATGATTGGCTCAAGAGAGGCGTGAGCAAAAGAACGATTCAACGAAATCAA
CAGACGATATTGGTGAAAATAGCCCATGAGGAGACAAAGCAGAGCTATGGTTATATTCGATTAACCAAATACTTACAAGCTCAGGGCATAAAAATGAGTA
TGTACGCTGTACGTCAGATAAAAGCGCTGAACCACCTGTATTGTAAGCGACACAAGCGTTTTAAAAGGACTACGAATAGTGACCATAATCGAGCGATCTA
TGAAAACCTGCTGGAGCAACAATTCTCAATGACTAGACCAAATCAAGCATGGTCAAGTGATATTACGTACATATGGACTGTTGAAGGATGGCTGTACTTA
GCAGCGGTAAAAGACCTTTACACGAAGCAAGTGGTTGGCTATAGCTTAAATGAGCGCATGACAACACAGCTTGTTTGTAATGCGCTAAATATGGCTATTC
ACAATCAAAAACCAACCAAAGAACTGATTGTGCATTCAGACAGAGGAAGTCAATATTGCAGCCATGAATATCGAAATATACTTGAGCAATATGGTTTTCA
AGGTTCAATGAGCAAGCGCGGAGACTGTTACGATAATGCACCGATTGAAAGCTTTTGGGGAATACTGAAAAATGAGTTAGTGCATCATTACAACTATCAA
ACCAGAGAAGAAGCCAAAGCAGATATTATAAAATACATTGAATTATTTTATAATCATCGAAGAATTCAAAAGGGTTTGGGTTTTAAGACACCAAATCAAA
TGGCCGAAGACTTTTATAAGTTGGCTGCCTAGAATCTCCCAAGGGAAAGTCTCCTGATAATTCAGCGTATATCA
TTAAAGCTGAAGCCATCAAATTAATTGAAGCCAATCAAGGCAATGTCTCGGAAACAGCCAGACAACTTAGCATTTCAATGCAAACTCTTTCAAATTGGAA
TACCAAAGCAAAGGCTGGAACTTTAGCAGGTACAAAACAGTATTCACCTGATCTAAACGCTCTACTCGAAGAAAATAAAAAACTCAAACAACAGCTCAAA
ATAGCTGAAATGGAACGTGAATTTTTAAAAAAGGCAGCAGCGTACTTTGCCAAAGAAAGTCAGTAAGGTACGCCTATATGAAACAAAAAAGATATTCTTT
TCCAATTACCTTAATGGCTCGATTACTTCATGTTTCAGTTTCATGTTTTTATGATTGGCTCAAGAGAGGCGTGAGCAAAAGAACGATTCAACGAAATCAA
CAGACGATATTGGTGAAAATAGCCCATGAGGAGACAAAGCAGAGCTATGGTTATATTCGATTAACCAAATACTTACAAGCTCAGGGCATAAAAATGAGTA
TGTACGCTGTACGTCAGATAAAAGCGCTGAACCACCTGTATTGTAAGCGACACAAGCGTTTTAAAAGGACTACGAATAGTGACCATAATCGAGCGATCTA
TGAAAACCTGCTGGAGCAACAATTCTCAATGACTAGACCAAATCAAGCATGGTCAAGTGATATTACGTACATATGGACTGTTGAAGGATGGCTGTACTTA
GCAGCGGTAAAAGACCTTTACACGAAGCAAGTGGTTGGCTATAGCTTAAATGAGCGCATGACAACACAGCTTGTTTGTAATGCGCTAAATATGGCTATTC
ACAATCAAAAACCAACCAAAGAACTGATTGTGCATTCAGACAGAGGAAGTCAATATTGCAGCCATGAATATCGAAATATACTTGAGCAATATGGTTTTCA
AGGTTCAATGAGCAAGCGCGGAGACTGTTACGATAATGCACCGATTGAAAGCTTTTGGGGAATACTGAAAAATGAGTTAGTGCATCATTACAACTATCAA
ACCAGAGAAGAAGCCAAAGCAGATATTATAAAATACATTGAATTATTTTATAATCATCGAAGAATTCAAAAGGGTTTGGGTTTTAAGACACCAAATCAAA
TGGCCGAAGACTTTTATAAGTTGGCTGCCTAGAATCTCCCAAGGGAAAGTCTCCTGATAATTCAGCGTATATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 67 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
MSKKHKTYTTEFKAEAIKLIEANQGNVSETARQLSISMQTLSNWNTKAKAGTLAGTKQYSPDLNALLEENKKLKQQLKIAEMEREFLKKAAAYFAKESQ*
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 303 aa | 321 | 1232 | + | No |
Description : Second part of the transposase
ORF sequence :
IFKKGSSVLCQRKSVRYAYMKQKRYSFPITLMARLLHVSVSCFYDWLKRGVSKRTIQRNQQTILVKIAHEETKQSYGYIRLTKYLQAQGIKMSMYAVRQI
KALNHLYCKRHKRFKRTTNSDHNRAIYENLLEQQFSMTRPNQAWSSDITYIWTVEGWLYLAAVKDLYTKQVVGYSLNERMTTQLVCNALNMAIHNQKPTK
ELIVHSDRGSQYCSHEYRNILEQYGFQGSMSKRGDCYDNAPIESFWGILKNELVHHYNYQTREEAKADIIKYIELFYNHRRIQKGLGFKTPNQMAEDFYK
LAA*
KALNHLYCKRHKRFKRTTNSDHNRAIYENLLEQQFSMTRPNQAWSSDITYIWTVEGWLYLAAVKDLYTKQVVGYSLNERMTTQLVCNALNMAIHNQKPTK
ELIVHSDRGSQYCSHEYRNILEQYGFQGSMSKRGDCYDNAPIESFWGILKNELVHHYNYQTREEAKADIIKYIELFYNHRRIQKGLGFKTPNQMAEDFYK
LAA*
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 67 | 1232 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKKHKTYTTEFKAEAIKLIEANQGNVSETARQLSISMQTLSNWNTKAKAGTLAGTKQYSPDLNALLEENKKLKQQLKIAEMEREFLKKGSSVLCQRKSV
RYAYMKQKRYSFPITLMARLLHVSVSCFYDWLKRGVSKRTIQRNQQTILVKIAHEETKQSYGYIRLTKYLQAQGIKMSMYAVRQIKALNHLYCKRHKRFK
RTTNSDHNRAIYENLLEQQFSMTRPNQAWSSDITYIWTVEGWLYLAAVKDLYTKQVVGYSLNERMTTQLVCNALNMAIHNQKPTKELIVHSDRGSQYCSH
EYRNILEQYGFQGSMSKRGDCYDNAPIESFWGILKNELVHHYNYQTREEAKADIIKYIELFYNHRRIQKGLGFKTPNQMAEDFYKLAA*
RYAYMKQKRYSFPITLMARLLHVSVSCFYDWLKRGVSKRTIQRNQQTILVKIAHEETKQSYGYIRLTKYLQAQGIKMSMYAVRQIKALNHLYCKRHKRFK
RTTNSDHNRAIYENLLEQQFSMTRPNQAWSSDITYIWTVEGWLYLAAVKDLYTKQVVGYSLNERMTTQLVCNALNMAIHNQKPTKELIVHSDRGSQYCSH
EYRNILEQYGFQGSMSKRGDCYDNAPIESFWGILKNELVHHYNYQTREEAKADIIKYIELFYNHRRIQKGLGFKTPNQMAEDFYKLAA*
Blast result :
Comments
ISAba22 is 64% (ORFA) and 77% (ORFB) aa similar to ISPar1. The third orf is a putative ORFAB transposase constructed in silico by a potential -1 frameshift.
References
1] Nigro, SJ and Hall, RM (2012) Direct submission
2] Zhou,H., Zhang,T., Yu,D., Pi,B., Yang,Q., Zhou,J., Hu,S. and Yu,Y. (2011) Antimicrob. Agents Chemother. 55 (10), 4506-4512
2] Zhou,H., Zhang,T., Yu,D., Pi,B., Yang,Q., Zhou,J., Hu,S. and Yu,Y. (2011) Antimicrob. Agents Chemother. 55 (10), 4506-4512