ISEc48
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ADUS01000070 | ND | Escherichia coli | Escherichia coli UMNK88 Escherichia coli RN587/1 Salmonella enterica subsp. enterica sv. Heidelberg SL476 |
DNA section
IS Length : 1336 bp
Ends
IR Length : 31/42
IRL : TGAGCTGGCCCCCTGAAATACCAGACAGTAGATGTATCTTAAAATAAGAG
IRR : TGTTCTTGCCCCCTTATTTTCAGACAGGGGTTGTATCTTAAGTTAACATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAAACAGTC | CGCATATTGATAATAT | ATTGACAGGC | 16 |
| TCAATATTTG | ATTTTTACAAAATTAG | TATTGCTCAT | 16 |
| GTTTTATGGT | GATGTGGAGTTTAAT | ATAGATGCCG | 15 |
DNA sequence
TGAGCTGGCCCCCTGAAATACCAGACAGTAGATGTATCTTAAAATAAGAGATAGGCTTGGATATATGTCTAACACTAGTTCTAATTTTGAGATGGCTGGC
GTTTTGTTAGGAAAAGAGGTGCGTAAACGTAAAACACCTCAGGAAAAAATCGCAATTATTCAACAGACTATGGAGCCGGGCATGACGGTGTCCCACGTGG
CGCGTTTGCATGGAATTCAGCCCAGTCTGTTGTTTAAGTGGAAGAAACAATATCAGGAAGGAAGCCTCACTGCTGTGGCTGCCGGTGAAGACGTTGTTCC
TGCTTCTGAACTGGCCGCTGCAATAAAGCAGATTAATCAGCTTCAGCGTTTACTGGGTAAGAAAACCATGGAAGTTGAGATCCTCAAAGAAGCGGTGGAG
TATGCACAATCGCGAAAATGGATAGCGCATGTGCCCTTGTTACCTTCGGACAAGGAATAGCCCCGGTAAGCCGTGTTCTGGGCGTGTCACGTGCGCAGTT
AACATTAAGGATGAAGGCATCTGATAACAAGCCAGATAAACGGCGTCAGAGGCGCGATGAGGCTGCGGATGCAGAAGTCTTATCGCGCATCCTGGATATC
ATTGGCGATATGCCAGCATACGGGTATCGCCGTGTCTGGGCGATACTCAGGCGGCAGTCCCGAAATGAAGGACTACCGTTCGTGAATGCAAAGCGCGTTT
ACCGGATAATGAGCGAAAATAGCCTGTTGTTGTTACATGACAAACCATCACGACGGCAGCGGGAACATAAAGGCCGAATATCGGTGAAGGAAAGCGACCA
GCGTTGGTGTTCTGATGGCTTTGAGTTCGGTTGCGATGATGGCGAAAAACTGCGTGTTACGTTCGTCCTGGACTGTTGCGATCGTGAAGCCATAGACTGG
GCCGCAAGCACGGGAGGTTACGATAAAGCGACAGTGCAGGATGTGATGTTAGGAGCGATAGAAAAACGCTTTGGCGATAAAGTACCGGAACAGTCCATCC
AGTGGCTGACAGATAACGGTTCAGCGTACAGAGCGCATGAAACACGGCAGTTCGCCAGAGAGCTGAATCTGAAGCCCTGCACAACGGCAATCAGTAGCCC
CCAGAGTAATGGAATGGCAGAACGGTTCGTGAAAACGATGAAGGAAGACTACATCGCGTTCATGCCGAAACCAAATGTAAGAACGGCATTGCATAACCTT
GCAGTGGCGATCGAGCATTACAATGAAAACCATCCGCATAGTGCGTTGGGTTATCGCTCTCCGCGAGAATATCGACGTCAGCGGGTAATGTTAACTTAAG
ATACAACCCCTGTCTGAAAATAAGGGGGCAAGAACA
GTTTTGTTAGGAAAAGAGGTGCGTAAACGTAAAACACCTCAGGAAAAAATCGCAATTATTCAACAGACTATGGAGCCGGGCATGACGGTGTCCCACGTGG
CGCGTTTGCATGGAATTCAGCCCAGTCTGTTGTTTAAGTGGAAGAAACAATATCAGGAAGGAAGCCTCACTGCTGTGGCTGCCGGTGAAGACGTTGTTCC
TGCTTCTGAACTGGCCGCTGCAATAAAGCAGATTAATCAGCTTCAGCGTTTACTGGGTAAGAAAACCATGGAAGTTGAGATCCTCAAAGAAGCGGTGGAG
TATGCACAATCGCGAAAATGGATAGCGCATGTGCCCTTGTTACCTTCGGACAAGGAATAGCCCCGGTAAGCCGTGTTCTGGGCGTGTCACGTGCGCAGTT
AACATTAAGGATGAAGGCATCTGATAACAAGCCAGATAAACGGCGTCAGAGGCGCGATGAGGCTGCGGATGCAGAAGTCTTATCGCGCATCCTGGATATC
ATTGGCGATATGCCAGCATACGGGTATCGCCGTGTCTGGGCGATACTCAGGCGGCAGTCCCGAAATGAAGGACTACCGTTCGTGAATGCAAAGCGCGTTT
ACCGGATAATGAGCGAAAATAGCCTGTTGTTGTTACATGACAAACCATCACGACGGCAGCGGGAACATAAAGGCCGAATATCGGTGAAGGAAAGCGACCA
GCGTTGGTGTTCTGATGGCTTTGAGTTCGGTTGCGATGATGGCGAAAAACTGCGTGTTACGTTCGTCCTGGACTGTTGCGATCGTGAAGCCATAGACTGG
GCCGCAAGCACGGGAGGTTACGATAAAGCGACAGTGCAGGATGTGATGTTAGGAGCGATAGAAAAACGCTTTGGCGATAAAGTACCGGAACAGTCCATCC
AGTGGCTGACAGATAACGGTTCAGCGTACAGAGCGCATGAAACACGGCAGTTCGCCAGAGAGCTGAATCTGAAGCCCTGCACAACGGCAATCAGTAGCCC
CCAGAGTAATGGAATGGCAGAACGGTTCGTGAAAACGATGAAGGAAGACTACATCGCGTTCATGCCGAAACCAAATGTAAGAACGGCATTGCATAACCTT
GCAGTGGCGATCGAGCATTACAATGAAAACCATCCGCATAGTGCGTTGGGTTATCGCTCTCCGCGAGAATATCGACGTCAGCGGGTAATGTTAACTTAAG
ATACAACCCCTGTCTGAAAATAAGGGGGCAAGAACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 369 bp | 122 aa | 92 | 460 | + | No |
Description : First part of the transposase
ORF sequence :
MAGVLLGKEVRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQPSLLFKWKKQYQEGSLTAVAAGEDVVPASELAAAIKQINQLQRLLGKKTMEVEILKE
AVEYAQSRKWIAHVPLLPSDKE
AVEYAQSRKWIAHVPLLPSDKE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 379 | 1299 | + | No |
Description : Second part of the transposase
ORF sequence :
DPQRSGGVCTIAKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKPDKRRQRRDEAADAEVLSRILDIIGDMPAYGYRRVWAILRRQSRNEGLP
FVNAKRVYRIMSENSLLLLHDKPSRRQREHKGRISVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCDREAIDWAASTGGYDKATVQDVMLGAIEKRFGD
KVPEQSIQWLTDNGSAYRAHETRQFARELNLKPCTTAISSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPREYRR
QRVMLT
FVNAKRVYRIMSENSLLLLHDKPSRRQREHKGRISVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCDREAIDWAASTGGYDKATVQDVMLGAIEKRFGD
KVPEQSIQWLTDNGSAYRAHETRQFARELNLKPCTTAISSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPREYRR
QRVMLT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1208 bp | 402 aa | 92 | 1299 | + | Yes |
Chemistry : DDE
ORF sequence :
MAGVLLGKEVRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQPSLLFKWKKQYQEGSLTAVAAGEDVVPASELAAAIKQINQLQRLLGKKTMEVEILKE
AVEYAQSRKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKPDKRRQRRDEAADAEVLSRILDIIGDMPAYGYRRVWAILRRQSRNEGLPFVNA
KRVYRIMSENSLLLLHDKPSRRQREHKGRISVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCDREAIDWAASTGGYDKATVQDVMLGAIEKRFGDKVPE
QSIQWLTDNGSAYRAHETRQFARELNLKPCTTAISSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPREYRRQRVM
LT
AVEYAQSRKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKPDKRRQRRDEAADAEVLSRILDIIGDMPAYGYRRVWAILRRQSRNEGLPFVNA
KRVYRIMSENSLLLLHDKPSRRQREHKGRISVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCDREAIDWAASTGGYDKATVQDVMLGAIEKRFGDKVPE
QSIQWLTDNGSAYRAHETRQFARELNLKPCTTAISSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPREYRRQRVM
LT
Blast result :
Comments
Genome in progress.
ISEc48 is 88% aa similar to ISEcl1.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISEc48 is 88% aa similar to ISEcl1.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2012)
2] Rasko,D., Redman,J., Daugherty,S.C., Tallon,L., Sadzewicz,L., Jones,K., Santana-Cruz,I. and Liu,X. (2011) Direct submission GenBank.
2] Rasko,D., Redman,J., Daugherty,S.C., Tallon,L., Sadzewicz,L., Jones,K., Santana-Cruz,I. and Liu,X. (2011) Direct submission GenBank.