ISfinder Mirror Site

ISEha1

Family IS200/IS605
Group IS200

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_014828	ND	Ethanoligenens harbinense	Ethanoligenens harbinense YUAN-3

DNA section

IS Length : 598 bp

Ends

Left end : ATCTCTGAGGGTTTAATTCCCCGCCGCTTGCGGCGTTAAATAAAAGGGTATAAAATTGGTATAACAAAAAACGAGAAGAGGGAAGAGATGAGTGAGTATA II struct. : Yes
Right end : AAACAACTTTACAAAGTTGAAACAATAGAACAACTCACGTTGTTTTAGCAAGGGTAGGGTTAGCATTGGATACCCCGCCGCTTGCGGCGGGGTGATTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TTGTCGTGTGCAATAT	TTTA	TTATCCAGTTGG	tcat
ACGTTTCCTCACACTA	TTTA	TTATTCCTCATA	tcat
TAGCGCGCTGAGAATA	TTTA	TGTCATTAAGGC	tcat
GTTTCGACTGACCGCT	TTTA	TTTTTTAGATGC	tcat

DNA sequence

ATCTCTGAGGGTTTAATTCCCCGCCGCTTGCGGCGTTAAATAAAAGGGTATAAAATTGGTATAACAAAAAACGAGAAGAGGGAAGAGATGAGTGAGTATA
TTCATAAATCTCACAATGTCTCAGTGCTGATGTACCATTTTGTATGCCCCGCTAAATATCGCAGAGCGATATTTAGCGAAGAAGTTACCAAAACACTCGT
AGAAGTGTGCGGAGGAATAAGCGAACGATTTGAGATGTACTTTGTTGAAATAGGTACAGATAAAAATCATGTGCATTTTCTAATACAATCAGTACCCAAG
TACAGCCCGACGAAAATAATCACAACAGTGAAAAGTATAACAGCACGAGAAATATTTTCTAAACATCCGGAAGTAAAAAAACAATTATGGGGTGGAGAAT
TATGGACGGATGGATATTTTGTGAATACGGTAAGCAAATTTGGCAGTGAAGAAGTAATAAAAAGATACTTGCAGAATCAGGGTAAGACAGAGGAGTATAA
ACAACTTTACAAAGTTGAAACAATAGAACAACTCACGTTGTTTTAGCAAGGGTAGGGTTAGCATTGGATACCCCGCCGCTTGCGGCGGGGTGATTCAT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
459 bp	152 aa	88	546	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MSEYIHKSHNVSVLMYHFVCPAKYRRAIFSEEVTKTLVEVCGGISERFEMYFVEIGTDKNHVHFLIQSVPKYSPTKIITTVKSITAREIFSKHPEVKKQL
WGGELWTDGYFVNTVSKFGSEEVIKRYLQNQGKTEEYKQLYKVETIEQLTLF

Blast result :

Comments

ISEha1 is 76% aa similar to ISCaa10.

References

1] ISfinder annotation (2012)
2] Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Misra,M., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Jeffries,C., Kyrpides,N., Ivanova,N., Mikhailova,N., Wang,A., Mouttaki,H., He,Z., Zhou,J., Hemme,C.L. and Woyke,T. (2010) direct submission GenBank.