ISSth2
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002888 | ND | Streptococcus salivarius | Streptococcus salivarius CCHSS3 Streptococcus salivarius 57.I |
DNA section
IS Length : 1539 bp
Ends
IR Length : 14/15
IRL : CAGATTGAAGATAAAGTCCTCAGAAGTTATACTTCTGGGGATTTTTCTTG
IRR : CAGAATGAAGACAAACATCGTTTTTGATGTTTGTCTTTTTTATATTCTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTAAGAAAA | AGCTGACGTTGCGTCAGCT | TTTATTTTCC | 19 |
| GCAATAAAAA | AGAAGAAAATCATTTTGATTTTCTTCT | TTTTTATTCA | 27 |
| GAAAATAAAG | AGAAGTAATCGTCTGATTACTTCT | TTTTGTCCGT | 24 |
| TCATAGTAAA | AAAGATCAACAATAATATGTTGATCTTT | TATTTTTACT | 28 |
| ACACAAAAAA | AGACAGACTACACCTGTAATCTGTCT | ATTTGTTTGA | 26 |
DNA sequence
CAGATTGAAGATAAAGTCCTCAGAAGTTATACTTCTGGGGATTTTTCTTGCTTTGAAGGAGTATAATAAAAGAAAAAGGTATTTGATGAGGTGTTCCTAT
GTTTCACAAAGAAAATCCCGAATACAATCGACGTCAAGTAGGATTCTATACGCTTGATGAACTTGTGCCTAAAGACCATTTTCTAAGAAAAGTCGAGGAA
ACGATTGATTTCTCTTTTATTTATGATCTTGTCGAAGATAGTTATTCCTCGGATAATGGTCGCCCTAGTCTAGACCCTGTTTTATTAGTTAAAATTCCGT
TGATTCAATGTTTTTATGGCATTCGTTCTATGCGCCAAACCATTAAAGATATTGAGGTGAATACGGCTTACCGATGGTTTCTTGGCTTATCTCTGGACGA
TAAGGTACCACATTTTACAACTTACGGTAAGAACTATAGCCGACGCTTTCAAAACAAGGAAGTGTTAGCACACATTTTTAGTCATGTGCTTCATCATGTT
TTGGAAGTGGGGCTCATTGATCCCTCAGAAATCTTCATTGATGGGACTCATATCAAGGCAGCTGCTAATAATCATAAGTACAAAACTGTTGTTATTGATC
AAAAAGCTAAATTCATGAGTGAACAACTGGAGATTGAGATTAATTTAGATAGGAAAAAACACGCAAAAAAGCTCTTAAAGCCCGCCGAAAAAAGCGAGGC
TAAACCTAAGAAACAATCGACGACGGACCCAGACAGTGGTTGGTTTCATAAGGGAGAACATAAGGAGGTCTTCGCTTACAATGCTCAAGTAGCTTGTGAT
AAACACGGCTGGGCCTTAGCTTATACGGTTGAAGCAGGAAACATCCATGATAGTCAGGCTTTCTCTGCTCTTTTTGTCAAACTAGAACCATTCTCTCCTG
AATTCATTATTGCGGATTCAGGCTATAAAACACCTAGTATTGCCAAGTTTCTTCTAGAAAAAGGGATTACACCAGTTTTCCCTTACACTAGACCAAGAGG
TAAGAAAGATTTTTTACGTCCAAAAGACTTTATCTATGACGAGCATTTTGATTGTTATCTTTGTCCAGAGAACAAGGTGTTAACTTATCGTACAACCACA
CGTGAAGGTTATCAAGAGTATAAAAGTAATCCTAAAATTTGTAAGACCTGTCCCTTGTTAGCTATTTGTACAGAAAGTCGCCAGAACCAAAAAGTTGTTG
TGCGTCATATTTGGAAAGATGCCCTAGAAGTCTGTGAAGATATTCGCCATCAAAGTGGTATGAAAGAACGGTATCAACACCGGAAAGAAACGATAGAACG
GTTATTCGGAACAGCTAAAGAATATCATAACTTACGTTATACCAGAGAAAAAGGAAAGTCCAAAATGGAAGATAAGGTTGGGCTTACTTTAGCGTGTCTA
AATATCAAAAAATTGGTAAAAATGATGACAGGAAAGACCTATAATTCTACCCAAATATCTCAATATTATTGGATTATCGGAGAATTAGGTAAGAATATAA
AAAAGACAAACATCAAAAACGATGTTTGTCTTCATTCTG
GTTTCACAAAGAAAATCCCGAATACAATCGACGTCAAGTAGGATTCTATACGCTTGATGAACTTGTGCCTAAAGACCATTTTCTAAGAAAAGTCGAGGAA
ACGATTGATTTCTCTTTTATTTATGATCTTGTCGAAGATAGTTATTCCTCGGATAATGGTCGCCCTAGTCTAGACCCTGTTTTATTAGTTAAAATTCCGT
TGATTCAATGTTTTTATGGCATTCGTTCTATGCGCCAAACCATTAAAGATATTGAGGTGAATACGGCTTACCGATGGTTTCTTGGCTTATCTCTGGACGA
TAAGGTACCACATTTTACAACTTACGGTAAGAACTATAGCCGACGCTTTCAAAACAAGGAAGTGTTAGCACACATTTTTAGTCATGTGCTTCATCATGTT
TTGGAAGTGGGGCTCATTGATCCCTCAGAAATCTTCATTGATGGGACTCATATCAAGGCAGCTGCTAATAATCATAAGTACAAAACTGTTGTTATTGATC
AAAAAGCTAAATTCATGAGTGAACAACTGGAGATTGAGATTAATTTAGATAGGAAAAAACACGCAAAAAAGCTCTTAAAGCCCGCCGAAAAAAGCGAGGC
TAAACCTAAGAAACAATCGACGACGGACCCAGACAGTGGTTGGTTTCATAAGGGAGAACATAAGGAGGTCTTCGCTTACAATGCTCAAGTAGCTTGTGAT
AAACACGGCTGGGCCTTAGCTTATACGGTTGAAGCAGGAAACATCCATGATAGTCAGGCTTTCTCTGCTCTTTTTGTCAAACTAGAACCATTCTCTCCTG
AATTCATTATTGCGGATTCAGGCTATAAAACACCTAGTATTGCCAAGTTTCTTCTAGAAAAAGGGATTACACCAGTTTTCCCTTACACTAGACCAAGAGG
TAAGAAAGATTTTTTACGTCCAAAAGACTTTATCTATGACGAGCATTTTGATTGTTATCTTTGTCCAGAGAACAAGGTGTTAACTTATCGTACAACCACA
CGTGAAGGTTATCAAGAGTATAAAAGTAATCCTAAAATTTGTAAGACCTGTCCCTTGTTAGCTATTTGTACAGAAAGTCGCCAGAACCAAAAAGTTGTTG
TGCGTCATATTTGGAAAGATGCCCTAGAAGTCTGTGAAGATATTCGCCATCAAAGTGGTATGAAAGAACGGTATCAACACCGGAAAGAAACGATAGAACG
GTTATTCGGAACAGCTAAAGAATATCATAACTTACGTTATACCAGAGAAAAAGGAAAGTCCAAAATGGAAGATAAGGTTGGGCTTACTTTAGCGTGTCTA
AATATCAAAAAATTGGTAAAAATGATGACAGGAAAGACCTATAATTCTACCCAAATATCTCAATATTATTGGATTATCGGAGAATTAGGTAAGAATATAA
AAAAGACAAACATCAAAAACGATGTTTGTCTTCATTCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1476 bp | 491 aa | 99 | 1574 | + | No |
Chemistry : DDE
ORF sequence :
MFHKENPEYNRRQVGFYTLDELVPKDHFLRKVEETIDFSFIYDLVEDSYSSDNGRPSLDPVLLVKIPLIQCFYGIRSMRQTIKDIEVNTAYRWFLGLSLD
DKVPHFTTYGKNYSRRFQNKEVLAHIFSHVLHHVLEVGLIDPSEIFIDGTHIKAAANNHKYKTVVIDQKAKFMSEQLEIEINLDRKKHAKKLLKPAEKSE
AKPKKQSTTDPDSGWFHKGEHKEVFAYNAQVACDKHGWALAYTVEAGNIHDSQAFSALFVKLEPFSPEFIIADSGYKTPSIAKFLLEKGITPVFPYTRPR
GKKDFLRPKDFIYDEHFDCYLCPENKVLTYRTTTREGYQEYKSNPKICKTCPLLAICTESRQNQKVVVRHIWKDALEVCEDIRHQSGMKERYQHRKETIE
RLFGTAKEYHNLRYTREKGKSKMEDKVGLTLACLNIKKLVKMMTGKTYNSTQISQYYWIIGELGKNIKKTNIKNDVCLHS
DKVPHFTTYGKNYSRRFQNKEVLAHIFSHVLHHVLEVGLIDPSEIFIDGTHIKAAANNHKYKTVVIDQKAKFMSEQLEIEINLDRKKHAKKLLKPAEKSE
AKPKKQSTTDPDSGWFHKGEHKEVFAYNAQVACDKHGWALAYTVEAGNIHDSQAFSALFVKLEPFSPEFIIADSGYKTPSIAKFLLEKGITPVFPYTRPR
GKKDFLRPKDFIYDEHFDCYLCPENKVLTYRTTTREGYQEYKSNPKICKTCPLLAICTESRQNQKVVVRHIWKDALEVCEDIRHQSGMKERYQHRKETIE
RLFGTAKEYHNLRYTREKGKSKMEDKVGLTLACLNIKKLVKMMTGKTYNSTQISQYYWIIGELGKNIKKTNIKNDVCLHS
Blast result :
Comments
ISSth2 is 85% aa similar to ISSag8.
The STOP codon is not in the IS, the transposase use the first STOP codon after the end of the IS.
The transposase in the database is cut at the end of the IS.
The STOP codon is not in the IS, the transposase use the first STOP codon after the end of the IS.
The transposase in the database is cut at the end of the IS.
References
1] ISfinder annotation (2012).
2] Geng,J., Huang,S.C., Li,S., Hu,S. and Chen,Y.Y. (2011) J. Bacteriol. 193 (19), 5596-5597.
2] Geng,J., Huang,S.C., Li,S., Hu,S. and Chen,Y.Y. (2011) J. Bacteriol. 193 (19), 5596-5597.