ISVal1
- Family IS5
- Group IS903
Isoform Synonym(s) ISV4R, ISV-NR
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HM362421 | ND | Vibrio alginolyticus | Vibrio parahaemolyticus 13-028/A3 plasmid pVPA3-1 Vibrio alginolyticus XSHD3 Vibrio parahaemoliticus AQ3 Vibrio cholerae 91 |
DNA section
IS Length : 1054 bp
Ends
IR Length : 19
IRL : GGCTTTGTTGCGTAATTCGATGGAACTAAATCAGGAAGTTACGATCTGAT
IRR : GGCTTTGTTGCGTAATTCGGACAGAAAACCAAGTCGTCCGAAATTGAATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATCTGTCAC | GTTTATTCCC | 0 |
DNA sequence
GGCTTTGTTGCGTAATTCGATGGAACTAAATCAGGAAGTTACGATCTGATCGGTTACATCAGTTAATCATCGTAGCCTTATGCCGAAACCTCGTTACAAA
ACAACTAACTGGAAGCAGTACAATAAAGCCTTAATCAACCGTGGTTCTCTGACTTTTTGGATTGATGAGGAAGCAATAGTCGAGTGGAAGCAAAACAAAC
AAGGCAAGCGTGGTAGACCTCGCCGATTCAGCGACTTAGCCATTACTACCGCACTGATGGTTAAGCGCGTCTTCTCCATGCCATTGAGAGCGCTGCAAGG
CTTTCTAGACTCAGTATTTAAGCTGGCTCACATCCCGCTTGTTTGCCCACACTACACCTGTATAAGCCGCCGAGCTAAGGAAGTTGAGGTTTCATTTAAA
ACCAAAACCAGAGGAGCAATACAACATCTGGCCATTGATGCCACTGGCCTCAAGGTTTATGGCGAAGGTGAATGGAAGGTCAAGAAGCATGGTACTGATG
GGAAGCGCAGAGTCTGGCGTAAGCTGCATATCGCAGTAGATACAAGCACCCACGAAATAGTCGCAGCAGAGCTGAGCTTATCTAACGTAACCGATGCCGA
AGTGCTCCCTAACTTACTCAAGCAGACACGTCGCAAAATCATTGAGATATCAGGTGATGGCGCTTATGACACAAGGAATTGCCATGATGCCATACGGATC
AAGCGAGCGGTTCCGCTTATCCCGCCACGAGAAGGGGCTGCCTTCTGGGAGCAAGGACACCCTCGCAATTTAGCAGTAGGTTGTCAGAAGCTCTACGGCT
CCAACAAGAAGTGGAAAAAGCGGTATGGTTATCACAAGCGTTCGCTCTCTGAAACAGCCATGTATCGCGTGAAGCAGTTGCTAGGTGGGAGGTTAAGCCT
GAGAAACTACAATTCTCAGGTTGGCGAAACCCACGCCATGATTAAAGCGCTGAACAAGCTTAGAGGGCTTGGTATGCCTGAAACTCAGTATATTGTTTAA
TAATTATTCAATTTCGGACGACTTGGTTTTCTGTCCGAATTACGCAACAAAGCC
ACAACTAACTGGAAGCAGTACAATAAAGCCTTAATCAACCGTGGTTCTCTGACTTTTTGGATTGATGAGGAAGCAATAGTCGAGTGGAAGCAAAACAAAC
AAGGCAAGCGTGGTAGACCTCGCCGATTCAGCGACTTAGCCATTACTACCGCACTGATGGTTAAGCGCGTCTTCTCCATGCCATTGAGAGCGCTGCAAGG
CTTTCTAGACTCAGTATTTAAGCTGGCTCACATCCCGCTTGTTTGCCCACACTACACCTGTATAAGCCGCCGAGCTAAGGAAGTTGAGGTTTCATTTAAA
ACCAAAACCAGAGGAGCAATACAACATCTGGCCATTGATGCCACTGGCCTCAAGGTTTATGGCGAAGGTGAATGGAAGGTCAAGAAGCATGGTACTGATG
GGAAGCGCAGAGTCTGGCGTAAGCTGCATATCGCAGTAGATACAAGCACCCACGAAATAGTCGCAGCAGAGCTGAGCTTATCTAACGTAACCGATGCCGA
AGTGCTCCCTAACTTACTCAAGCAGACACGTCGCAAAATCATTGAGATATCAGGTGATGGCGCTTATGACACAAGGAATTGCCATGATGCCATACGGATC
AAGCGAGCGGTTCCGCTTATCCCGCCACGAGAAGGGGCTGCCTTCTGGGAGCAAGGACACCCTCGCAATTTAGCAGTAGGTTGTCAGAAGCTCTACGGCT
CCAACAAGAAGTGGAAAAAGCGGTATGGTTATCACAAGCGTTCGCTCTCTGAAACAGCCATGTATCGCGTGAAGCAGTTGCTAGGTGGGAGGTTAAGCCT
GAGAAACTACAATTCTCAGGTTGGCGAAACCCACGCCATGATTAAAGCGCTGAACAAGCTTAGAGGGCTTGGTATGCCTGAAACTCAGTATATTGTTTAA
TAATTATTCAATTTCGGACGACTTGGTTTTCTGTCCGAATTACGCAACAAAGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 80 | 1000 | + | No |
Chemistry : DDE
ORF sequence :
MPKPRYKTTNWKQYNKALINRGSLTFWIDEEAIVEWKQNKQGKRGRPRRFSDLAITTALMVKRVFSMPLRALQGFLDSVFKLAHIPLVCPHYTCISRRAK
EVEVSFKTKTRGAIQHLAIDATGLKVYGEGEWKVKKHGTDGKRRVWRKLHIAVDTSTHEIVAAELSLSNVTDAEVLPNLLKQTRRKIIEISGDGAYDTRN
CHDAIRIKRAVPLIPPREGAAFWEQGHPRNLAVGCQKLYGSNKKWKKRYGYHKRSLSETAMYRVKQLLGGRLSLRNYNSQVGETHAMIKALNKLRGLGMP
ETQYIV
EVEVSFKTKTRGAIQHLAIDATGLKVYGEGEWKVKKHGTDGKRRVWRKLHIAVDTSTHEIVAAELSLSNVTDAEVLPNLLKQTRRKIIEISGDGAYDTRN
CHDAIRIKRAVPLIPPREGAAFWEQGHPRNLAVGCQKLYGSNKKWKKRYGYHKRSLSETAMYRVKQLLGGRLSLRNYNSQVGETHAMIKALNKLRGLGMP
ETQYIV
Blast result :
Comments
ISVal1 is 98% aa similar to ISVa2.
Additionnal accession number : KM067908.1.
Vibrio parahaemolyticus is the causative agent of AHPND (acute hepatopancreatic necrosis disease, also known as early mortality syndrome, EMS) in shrimp. ISVal1 flank the Photorhabdus insect related (Pir) toxin-like genes (pirA and pirB), and the composite transposon was desitnated as Tn6264.
Additionnal accession number : KM067908.1.
Vibrio parahaemolyticus is the causative agent of AHPND (acute hepatopancreatic necrosis disease, also known as early mortality syndrome, EMS) in shrimp. ISVal1 flank the Photorhabdus insect related (Pir) toxin-like genes (pirA and pirB), and the composite transposon was desitnated as Tn6264.
References
1] Ren, C., Jiang,X., Sun,H., Luo,P., Chen,C., Zhao,Z. and Hu,C. (2012) Ann. Microbiol. 62 (1), 69-75
2] Ren, C. (2010) Direct submmission
3] Terai, A., Baba, K., Shirai, H., Yoshida, O., Takeda, Y., and Nishibuchi, M. (1991) J. Bacteriol. 173, 5036-5046.
4] Han,J.E., Tang,K.F., Tran,L.H. and Lightner,D.V.(2015) Dis. Aquat. Org. 113, 33-40
2] Ren, C. (2010) Direct submmission
3] Terai, A., Baba, K., Shirai, H., Yoshida, O., Takeda, Y., and Nishibuchi, M. (1991) J. Bacteriol. 173, 5036-5046.
4] Han,J.E., Tang,K.F., Tran,L.H. and Lightner,D.V.(2015) Dis. Aquat. Org. 113, 33-40