ISfinder Mirror Site

ISEc41

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_004431	ND	Escherichia coli	Escherichia coli CFT073

DNA section

IS Length : 1841 bp

Ends

Left end : CCTTCCTCCACTGGGTACTCCCCCCAACGTAGGCGGGAGGGGAATTTGGGCTGTTAAGCCCGCAGTTTATCAGCCACGCTGATTCTGAATATACTTTTTC II struct. : Yes
Right end : CCGTCGAGCGAGGAACCGGGGAATGCGGCAACGTATTGCTACCCTGACAAGGGAATCCCCCTCCTGAGTCGCTTTGCGCGAAGGTGGGGGAGGATGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ATTCGATTTCCTGAG	CCAT	GGCCTTTACT	TCAA

DNA sequence

CCTTCCTCCACTGGGTACTCCCCCCAACGTAGGCGGGAGGGGAATTTGGGCTGTTAAGCCCGCAGTTTATCAGCCACGCTGATTCTGAATATACTTTTTC
ACAACTTCCAGCGGTGCTCCACCACATGAACCGATGAAATAAGAGCGTGACCAGAGTACGGGCTTTGAATACGCGGCGCGCAAATCAATAAAATCATTCC
GCAGCCGCCTACTGGTAACGGACTTAAGGCTGTTTACCAGCTTTGTGAGTTGCACCAACGGTGGGTATTCAATCAGCATGTGAACATGGTCTGCCTCGCC
GTCACATTCCTTTAACTCGGCGCCAAAGTCCGCGCATACCTCCGCTACATACTGACGCATGTTTGCATGATGCAATTCACCAAAAACTGGACGGCGGTAT
TTTGTCATAAAAACAACGTGAACATGCAACAGGTAAGCTGAATAGCGTGAACGATTGATTTCGCACACGTATTGACCCTCCCAAAAAAATGTGGATATAT
TGTACATTCTAAACAACCACATACTGAATGCAAAATGTTAATCCAGAAAGCATATAAATACAGGCTCTACCCCACTGACCAGCAGGCGCAAAGGTTGCGT
CAGTTGTGCGGTTGCGCTCGCTTTGTCTGGAACTATGCACTGAATGAAACACTGAGCATTCATGACGCGGGCGGAAAAATTCCGTCAGCTTTCGACCTGA
ATAAACGACTTACGGGCTGGAAAAAACTCCCTGAGCTGGCCTTTCTTTCTGAGGGTTACACTGACAACCTCCAGCAGAAACTGAAAGACCTGCGGAGCGC
ATGGGATCGCTGTTTTGATAAATCCCTGACGGCTGAAAAGCCCGTTTTCAAAAAGAAAACCAAAGGCTGCGACTCTATCCGGTTCGTCAATTTCAGCAAA
TATTGTGGGCTGGATTATGGCCGTGTGAAACTCCCGTCAGGACTGGGCTGGGTTAAATTTCGTCAGTCCCGGAAGATTGAAGGCGTCATTAAAAATTGCA
CCATCAGCCAGCATGCCGGACACTGGTATGTGTCTTTTCAGGTCGAATTAGCTGTTACCGATCCGATTCACGCATCGACCAGCGCAATAGGCCTGGATGC
AGGTATAACGAAGCTGGCAACACTTTCAGACGGCACGGTCTTTGAACCGGTAAACAGCTTAAAAAAAAATCAGGATAAGCTGGCTCGCTTACAGCGCCGT
CTGGCCCGGATGGTTAAATTCTCCGCCAACTGGAAAAAGCAGAAAGCGAAGATTTCCCGTTTCCACTCTCATATCGCAAACATCCGCCGCGATTACCTTC
ATAAAACCACGACGACGATCAGCAAAAACCACGCGATGATAGTCATTGAGGATTTAAAGGTTTCGAACATGTCAAAATCAGCAGCGGGTACGGTAGACCA
GCCGGGGCGCAACGTCGCGGCAAAATCAGGTCTTAACCGGGCGATACTGGATCAGGGCTGGGCAGAAATGCGCCGCCAGCTCGAATATAAGCAGGCATGG
CGCGGCGGTGATGTACTGGCGATTAATCCGGCGTACACCAGTCAGAAATGCGCATGTTGTGGGCATACATCGAAAAATAACCGCAGGACGCAGGCGAGCT
TTATCTGTACCGCGTGCGGCTATACGGCAAACGCCGATGTAAACGGCGCTCGCAATATATTAACAGCAGGTTTTGAAGTCATGGCGGCAGGACAAATATT
GCCGTCAGTCCGTAAAGGACGCGCCCGTAAGGCGGCGTAAGCCGTCGAGCGAGGAACCGGGGAATGCGGCAACGTATTGCTACCCTGACAAGGGAATCCC
CCTCCTGAGTCGCTTTGCGCGAAGGTGGGGGAGGATGTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
453 bp	150 aa	522	70	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MWLFRMYNISTFFWEGQYVCEINRSRYSAYLLHVHVVFMTKYRRPVFGELHHANMRQYVAEVCADFGAELKECDGEADHVHMLIEYPPLVQLTKLVNSLK
SVTSRRLRNDFIDLRAAYSKPVLWSRSYFIGSCGGAPLEVVKKYIQNQRG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1206 bp	401 aa	535	1740	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLIQKAYKYRLYPTDQQAQRLRQLCGCARFVWNYALNETLSIHDAGGKIPSAFDLNKRLTGWKKLPELAFLSEGYTDNLQQKLKDLRSAWDRCFDKSLTA
EKPVFKKKTKGCDSIRFVNFSKYCGLDYGRVKLPSGLGWVKFRQSRKIEGVIKNCTISQHAGHWYVSFQVELAVTDPIHASTSAIGLDAGITKLATLSDG
TVFEPVNSLKKNQDKLARLQRRLARMVKFSANWKKQKAKISRFHSHIANIRRDYLHKTTTTISKNHAMIVIEDLKVSNMSKSAAGTVDQPGRNVAAKSGL
NRAILDQGWAEMRRQLEYKQAWRGGDVLAINPAYTSQKCACCGHTSKNNRRTQASFICTACGYTANADVNGARNILTAGFEVMAAGQILPSVRKGRARKA
A

Blast result :

Comments

ISEc41 is 66% aa (TnpA : the transposase) and 70% aa (TnpB) similar to IS609.

References

1] ISfinder annotation (2012)
2] Welch,R.A., Burland,V., Plunkett,G. III, Redford,P., Roesch,P., Rasko,D., Buckles,E.L., Liou,S.R., Boutin,A., Hackett,J., Stroud,D., Mayhew,G.F., Rose,D.J., Zhou,S., Schwartz,D.C., Perna,N.T., Mobley,H.L., Donnenberg,M.S. and Blattner,F.R. (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 17020-1702