ISEc39
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001855 | ND | Escherichia coli | Escherichia coli O83:H1 NRG 857C |
DNA section
IS Length : 1315 bp
Ends
IR Length : 22/29
IRL : GAGACTGTAATTAAAATTGTGTAATTGCCTGTTTTTGATATGTTCACTCC
IRR : GACCCTGTAAATAATTCTGTGTAACTGCCGCCGTATTAAAGGTGCTCGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATGTGAATA | CAATAAAA | TTATATTTAG | 8 |
DNA sequence
GAGACTGTAATTAAAATTGTGTAATTGCCTGTTTTTGATATGTTCACTCCAACAACGGAGACAGGCAAACGATGGACGAAAAGAAACTTAAAGCCCTTGC
GGCTGAACTGGCCAAAGGCCTTAAAACCGAAGCTGACCTCAACCAGTTTTCACGCATGTTGACAAAGCTTACCGTCGAAACGGCGCTCAATGCAGAACTG
ACTGACCATCTCGGTCATGAGAAAAATGCTCCTAAATCAGGCTCTAATACCCGTAATGGCTACTCGTCAAAAACGGTATTGTGCGATGATGGCGAGATCG
AACTTAACACGCCGCGTGACCGTGAAAATACCTTCGAACCGCAACTAATTAAGAAGCATCAGACACGCATCACCCAGATGGACAGCCAGATTTTGTCCCT
GTATGCCAAAGGCATGACTACGCGGGAAATCGTTGCCACGTTCAAAGAAATGTACGACGCCGATGTCTCGCCCACGCTGATATCCAAAGTGACCGATGCG
GTTAAAGAACAGGTCTCTGAATGGCAAAATCGACCGTTGGATGCACTGTATCCCATTGTTTATCTTGACTGTATTGTGGTTAAGGTTCGTCACGGCGGGA
GTGTCATTAACAAAGCGGTGTTCCTCGCGCTGGGCATCAATACCGACGGTCAGAAAGAGCTGCTGGGTATGTGGCTGGCAGAAAATGAAGGTGCAAAGTT
CTGGCTGGGTGTACTGACTGAGCTGAAAAATCGTGGCCTTCAGGACATCCTGATTGCCTGCGTGGACGGCCTGAAAGGCTTCCCGGAAGCGATAAATAGC
GTGTACCCGCAGACGCATATTCAGCTCTGCATCATCCATATGGTGCGCAACAGCCTGAAATTCGTGTCGTGGAAGGATTACAAAGCCGTCACCAGCGGGC
TGAAAGCGGTATATCAGGCCCCGACAGAGGAAGCGGCGTTGATGGCGTTGGATAAGTTTGCAGGCGTCTGGGATGAAAAATACCCGCAAATCAGCAAGAG
CTGGCGCACACACTGGGAAAATCTCAACACCTTCTTCGGTTACCCGCCGGATATCCGCAAGGCCATCTACACGACGAATGCCATCGAATCGCTGAACAGC
GTCATTCGGCAGGCGATAAAGAAGCGCAAGGTGTTCCCAACGGACGACTCGGTGCGAAAAGTGATTTATCTGGCGATACAGTCGGCGTCAAAAAAATGGA
GTATGCCAATCCAGAACTGGCGGCTGGCAATGAGCCGTTTTATTATCGAGTTTGGTGACCGCCTTAGCGAGCACCTTTAATACGGCGGCAGTTACACAGA
ATTATTTACAGGGTC
GGCTGAACTGGCCAAAGGCCTTAAAACCGAAGCTGACCTCAACCAGTTTTCACGCATGTTGACAAAGCTTACCGTCGAAACGGCGCTCAATGCAGAACTG
ACTGACCATCTCGGTCATGAGAAAAATGCTCCTAAATCAGGCTCTAATACCCGTAATGGCTACTCGTCAAAAACGGTATTGTGCGATGATGGCGAGATCG
AACTTAACACGCCGCGTGACCGTGAAAATACCTTCGAACCGCAACTAATTAAGAAGCATCAGACACGCATCACCCAGATGGACAGCCAGATTTTGTCCCT
GTATGCCAAAGGCATGACTACGCGGGAAATCGTTGCCACGTTCAAAGAAATGTACGACGCCGATGTCTCGCCCACGCTGATATCCAAAGTGACCGATGCG
GTTAAAGAACAGGTCTCTGAATGGCAAAATCGACCGTTGGATGCACTGTATCCCATTGTTTATCTTGACTGTATTGTGGTTAAGGTTCGTCACGGCGGGA
GTGTCATTAACAAAGCGGTGTTCCTCGCGCTGGGCATCAATACCGACGGTCAGAAAGAGCTGCTGGGTATGTGGCTGGCAGAAAATGAAGGTGCAAAGTT
CTGGCTGGGTGTACTGACTGAGCTGAAAAATCGTGGCCTTCAGGACATCCTGATTGCCTGCGTGGACGGCCTGAAAGGCTTCCCGGAAGCGATAAATAGC
GTGTACCCGCAGACGCATATTCAGCTCTGCATCATCCATATGGTGCGCAACAGCCTGAAATTCGTGTCGTGGAAGGATTACAAAGCCGTCACCAGCGGGC
TGAAAGCGGTATATCAGGCCCCGACAGAGGAAGCGGCGTTGATGGCGTTGGATAAGTTTGCAGGCGTCTGGGATGAAAAATACCCGCAAATCAGCAAGAG
CTGGCGCACACACTGGGAAAATCTCAACACCTTCTTCGGTTACCCGCCGGATATCCGCAAGGCCATCTACACGACGAATGCCATCGAATCGCTGAACAGC
GTCATTCGGCAGGCGATAAAGAAGCGCAAGGTGTTCCCAACGGACGACTCGGTGCGAAAAGTGATTTATCTGGCGATACAGTCGGCGTCAAAAAAATGGA
GTATGCCAATCCAGAACTGGCGGCTGGCAATGAGCCGTTTTATTATCGAGTTTGGTGACCGCCTTAGCGAGCACCTTTAATACGGCGGCAGTTACACAGA
ATTATTTACAGGGTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1209 bp | 402 aa | 72 | 1280 | + | No |
Chemistry : DDE
ORF sequence :
MDEKKLKALAAELAKGLKTEADLNQFSRMLTKLTVETALNAELTDHLGHEKNAPKSGSNTRNGYSSKTVLCDDGEIELNTPRDRENTFEPQLIKKHQTRI
TQMDSQILSLYAKGMTTREIVATFKEMYDADVSPTLISKVTDAVKEQVSEWQNRPLDALYPIVYLDCIVVKVRHGGSVINKAVFLALGINTDGQKELLGM
WLAENEGAKFWLGVLTELKNRGLQDILIACVDGLKGFPEAINSVYPQTHIQLCIIHMVRNSLKFVSWKDYKAVTSGLKAVYQAPTEEAALMALDKFAGVW
DEKYPQISKSWRTHWENLNTFFGYPPDIRKAIYTTNAIESLNSVIRQAIKKRKVFPTDDSVRKVIYLAIQSASKKWSMPIQNWRLAMSRFIIEFGDRLSE
HL
TQMDSQILSLYAKGMTTREIVATFKEMYDADVSPTLISKVTDAVKEQVSEWQNRPLDALYPIVYLDCIVVKVRHGGSVINKAVFLALGINTDGQKELLGM
WLAENEGAKFWLGVLTELKNRGLQDILIACVDGLKGFPEAINSVYPQTHIQLCIIHMVRNSLKFVSWKDYKAVTSGLKAVYQAPTEEAALMALDKFAGVW
DEKYPQISKSWRTHWENLNTFFGYPPDIRKAIYTTNAIESLNSVIRQAIKKRKVFPTDDSVRKVIYLAIQSASKKWSMPIQNWRLAMSRFIIEFGDRLSE
HL
Blast result :
Comments
ISEc39 is 92% aa similar to IS285.
References
1] ISfinder annotation (2012)
2] Eaves-Pyles,T., Allen,C.A., Taormina,J., Swidsinski,A., Tutt,C.B., Jezek,G.E., Islas-Islas,M. and Torres,A.G. (2008) Int. J. Med. Microbiol. 298 (5-6), 397-409.
3] Nash,J.H., Villegas,A., Kropinski,A.M., Aguilar-Valenzuela,R., Konczy,P., Mascarenhas,M., Ziebell,K., Torres,A.G., Karmali,M.A. and Coombes,B.K. (2010) BMC Genomics 11 (1), 667.
2] Eaves-Pyles,T., Allen,C.A., Taormina,J., Swidsinski,A., Tutt,C.B., Jezek,G.E., Islas-Islas,M. and Torres,A.G. (2008) Int. J. Med. Microbiol. 298 (5-6), 397-409.
3] Nash,J.H., Villegas,A., Kropinski,A.M., Aguilar-Valenzuela,R., Konczy,P., Mascarenhas,M., Ziebell,K., Torres,A.G., Karmali,M.A. and Coombes,B.K. (2010) BMC Genomics 11 (1), 667.