ISAsp10

  • Family IS4
  • Group IS10
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Anabaena sp.
Anabaena sp. 90
DNA section
IS Length : 1270 bp

Ends


IR Length : 17

IRL : CTGACGGGATGACGAAATAGTCTAAAAAGCTTATCGTATACAGATTATAC
IRR : CTGACGGGATGACGAAAATGGTTAAGATGCAGACAGAATAAGCCTCATAG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

CTGACGGGATGACGAAATAGTCTAAAAAGCTTATCGTATACAGATTATACAGATTTATGGTAAAAAAGATAGAAGCACTTTTCTCAATAAGGACTATCTA
TTGATAATGTTACCTGAATTCTACAAAACACACCTAAAAAATCAATTGAATCTGGCAGAGTATATCTTGCTCAAAATTATGATTATTCTGCTGCAATCAC
TGAAAAAAGTAAGTTTAGAAGCTTTGGCTACTGCTCTACCAATTCCGATTAAATTTGAAAGTAGGAAAAGAAAAATACAGAGATTTCTATCTTTACCAAA
CCTAACAATTGAGAAAATCTGGTTTCCGATTGTAAAAACATTTTTAGAAACATATTTGAAGGATGAAGAATTAATTTATGTAGTGATGGATAGGACGGCT
TGGGGACGCATAAATTTATTCATGATTAGTATAGTTTGGGATCAAAGGGCATTTCCTGTATATTTTGAGTTATTGCCAAAGTTAGGCAGCAGTAACTTTG
AGGAGCAATCACGAATATTGAAGAAAATTTTACCCATTTTTAATAACTATAAAATCTGCGGATTAGGTGATAGAGAATTTTGTTCTATAAAACTAGCAAA
TTGGTTAAAAGACCAGGGCTTATGCTTTTGTACAAGACTTAAAAAGAGTGAATTTGTAGAAATCAAAAAAGAAATTTGGGTGGATTTAAATAATCTAGGT
TTATCACCAGGAGTTGCTTTCTTTTTAGCCGGAGTTAAAGTTACAAAAAAACATGGTTTTACAAGTTTTAATGTTGCCTGTAAATGGAAACGCAAGATAT
TAGGAGTAGCCCCAAAGGAAGGGTGGTTTATTTTAACTAATTTAGATAGTTTGGAATTAGCAATTTCCGCCTATAAGAAGCGATTTTGTATAGAAGAAAT
GTTCCGTGATTTTAGAAGTGGCGGTTATAATTTGGAAGACACAAAAGTTACAAATAATCGGTTAATATCTATAATTTTATTAATATCAATTGCTTATACG
AGTGCCACAATACAAGGGCAAGAAATTAAACGCAAAGGTATCCAGAAATATATTGGTCGAGTGAAAGAATATGGACGTACAGAAAGAAGACATAGTAGTT
TTTATATTGGATTATATGCCCAATCTTGGGTTAATTTCATAGATAATTGTAGGGAATTAGTTAGGTCACCATTGAGATTATCTCCTAATAAGTGGAAGTA
TTATCAACAGGGTGAGAGAGCTATGAGGCTTATTCTGTCTGCATCTTAACCATTTTCGTCATCCCGTCAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1143 bp380 aa1071249+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLPEFYKTHLKNQLNLAEYILLKIMIILLQSLKKVSLEALATALPIPIKFESRKRKIQRFLSLPNLTIEKIWFPIVKTFLETYLKDEELIYVVMDRTAWG
RINLFMISIVWDQRAFPVYFELLPKLGSSNFEEQSRILKKILPIFNNYKICGLGDREFCSIKLANWLKDQGLCFCTRLKKSEFVEIKKEIWVDLNNLGLS
PGVAFFLAGVKVTKKHGFTSFNVACKWKRKILGVAPKEGWFILTNLDSLELAISAYKKRFCIEEMFRDFRSGGYNLEDTKVTNNRLISIILLISIAYTSA
TIQGQEIKRKGIQKYIGRVKEYGRTERRHSSFYIGLYAQSWVNFIDNCRELVRSPLRLSPNKWKYYQQGERAMRLILSAS

 

Blast result :
Comments
ISAsp10 is 72% aa similiar to ISNpu4.
There are 1 complete copy and 1 truncated copy in the genome.
References
1] WANG Hao (2011) Direct submission