ISfinder Mirror Site

ISCsa5

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009437	ND	Caldicellulosiruptor saccharolyticus	Caldicellulosiruptor kronotskyensis Anaerocellum thermophilis Caldicellulosiruptor obsidiansis Caldicellulosiruptor saccharolyticus Caldicellulosiruptor hydrothermalis

DNA section

IS Length : 1775 bp

Ends

Left end : ACAAACATTCTGCAAGCAGTCTCCATCTTCTGTAAGATGGAGATGAATTGCAATTGATAAATATTTGGTTTCGGTATACAATAAAACTGGTGATGACAAT II struct. : Yes
Right end : CTGTGGAGATTGGACCTCTACTACTGGGGATACAGTCCCAGTAGCAAGTCTGGTCGATGAAACAGGAAGCCTCCACCTCTTTAGGTGGATGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CTGAGAAAGT	TTTA	TTATTACTCT	tcac

DNA sequence

ACAAACATTCTGCAAGCAGTCTCCATCTTCTGTAAGATGGAGATGAATTGCAATTGATAAATATTTGGTTTCGGTATACAATAAAACTGGTGATGACAAT
GAAATTTGATACTAATAAACATTCAGTATTTCTACTATACTATCATTTGATTTTGGTGACAAAATACAGAAGAGATGTGATAGATGAGAAAATTTCAAAA
AGACTAAAAGAAATTTTTGAGTATATTCAACCCAATTACAACATAACCCTGGTTGAGTGGAATCATGATAAAGACCATGTGCATGTTTTGTTCAAGGCAA
CTCCTACAACATCACTTTCCAAGTTTATAAATGCTTATAAAAGTGCTTCATCAAGGCTTATAAAAAAGGAATTTCCTGAAATTAGGCAAAAACTATGGAA
GGAATATTTTTGGTCAAGAAGTTATTGTTTGCTAACAAGCGGTGGTGCACCGGTTGAAGTGATTAGAAAATACATTGAAAGTCAAGGTGAGAAAAGAAAA
TGTTGAAAGCATATAGGTATCAAATATATCCAACTAAAGCACAGGAAGAATTCTTTGAAAAGACATTTGGTTGCTGTAGATTTGTATGGAACAAGATGTT
AGAAGAAAAACTGGAAGCTTTGAAACAAGGTAAAAAAGTTCCAAGAATAACTCCAGCAAGGTATAAAAAAGAATATCCTTTTCTGAAGGAAGTGGATAGT
CTTGCACTTGCAAATGTTCAGCTTGAGCAGGAAAAGGCATTCAAAGACTTCTTAAAAAACTCAAAGCATTTTGGAATGCCAAAGTTTAAGAGGAAAAAAG
ACAAGCAGTCATACACAACGAATAACCAACAAACTAAGAGCGGAAACGAAACAATTAGGGTAGATTTTGAGAAGGGGATTTTGTATTTACCGAAGGTAAA
AAGTGGAATAAAAGCAGTATTTCACAGGGAGTTTGAAGGAAAAATAAAATCAGCAACAGTTGTTAAAACAAAAGCAGGCAGGTATTATGTAAGCATACTT
GTAGATGTGCAGGACCCAAGAAATAAGAGGGAAAATCGAAATTTTGAGAATGAGGTAATCAAACGAGAGAATTTCATCTGTGGAATAGATACAGGGCTTA
TAAGTTTTGTTACAATTGCAAATGATACAGGGATTTCGAAAATTGAATACCCAAAGTATCTGATTAAAGCTGAAAAGAGACTAAGAAGGTTGCACAGACA
GTTGTCAAGAAAGAAAAAAGGTTCTAAAAACTGGGAGAAAGCAAGAGAAAGACTTGCAAAGGAATATGAATATGTAAAGAACTCAAGGGATGACTTTTTG
CATAAGTTATCGAGGACCATCATAGACGAGAACCAAGTCGTGGTGGTCGAAAGTTTGAACGTAAAGGGACTATCAAAAACAAGATTATCGAAACACATTC
TTGATAGTTCATGGTCAAAATTTTTGAAATATCTCAAATACAAGGCAGATTGGTATGGCAGGAAAGTCATAGAGGCAGATAGGAGATTTCCATCTTCGAA
AATGTGTAGCAGATGTGGATACATCAACAAAGAGCTAAAGTTGAAAGATAGAGTGTGGAGATGTCCGAAATGCGGAACAGTCCATGATAGAGATGAAAAT
GCTGCAAAGAACCTGAGAAACTATGGAATTTTTTGTATAAGCAATAAGTAGGGTGGGGACCACCCGAATTTACGCCTGTGGAGATTGGACCTCTACTACT
GGGGATACAGTCCCAGTAGCAAGTCTGGTCGATGAAACAGGAAGCCTCCACCTCTTTAGGTGGATGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
453 bp	150 aa	54	506	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MINIWFRYTIKLVMTMKFDTNKHSVFLLYYHLILVTKYRRDVIDEKISKRLKEIFEYIQPNYNITLVEWNHDKDHVHVLFKATPTTSLSKFINAYKSASS
RLIKKEFPEIRQKLWKEYFWSRSYCLLTSGGAPVEVIRKYIESQGEKRKC

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1152 bp	383 aa	500	1651	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLKAYRYQIYPTKAQEEFFEKTFGCCRFVWNKMLEEKLEALKQGKKVPRITPARYKKEYPFLKEVDSLALANVQLEQEKAFKDFLKNSKHFGMPKFKRKK
DKQSYTTNNQQTKSGNETIRVDFEKGILYLPKVKSGIKAVFHREFEGKIKSATVVKTKAGRYYVSILVDVQDPRNKRENRNFENEVIKRENFICGIDTGL
ISFVTIANDTGISKIEYPKYLIKAEKRLRRLHRQLSRKKKGSKNWEKARERLAKEYEYVKNSRDDFLHKLSRTIIDENQVVVVESLNVKGLSKTRLSKHI
LDSSWSKFLKYLKYKADWYGRKVIEADRRFPSSKMCSRCGYINKELKLKDRVWRCPKCGTVHDRDENAAKNLRNYGIFCISNK

Blast result :

Comments

ISCsa5 is 85% (ORFA : the transposase) and 64% (ORFB) aa similar to ISEfa4.

References

1] ISfinder annotation (2011)
2] Miriam Land (2011) Direct submission.
3] Blumer-Schuette,S.E., Ozdemir,I., Mistry,D., Lucas,S., Lapidus,A., Cheng,J.F., Goodwin,L.A., Pitluck,S., Land,M.L., Hauser,L.J., Woyke,T., Mikhailova,N., Pati,A., Kyrpides,N.C., Ivanova,N., Detter,J.C., Walston-Davenport,K., Han,S., Adams,M.W. and Kelly,R.M. (2011) J. Bacteriol. 193 (6), 1483-1484.