ISBsu1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_014479 | ND | Bacillus subtilis | Bacillus amyloliquefaciens FZB42 Bacillus subtilis subsp. spizizenii W23 Bacillus subtilis subsp. spizizenii TU-B-10 Bacillus subtilis subsp. subtilis RO-NN-1 |
DNA section
IS Length : 1289 bp
Ends
IR Length : 32/42
IRL : TGGGACTGACCCCATAATATGAGACAAATAAAAAACACCTTCTAGTTTGA
IRR : TGGGACTGACCCCCGTTTTTGAGACAGGGATCAAAACACCTTTTAAACAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCGGAAAAT | TTAAAAGCAG | 0 | |
| ACCGGCACAT | TTTGCCGGAT | 0 | |
| CCATTTCTGT | TTCCGAAGGC | 0 | |
| AGCCGCTCAA | TC | TTCGGCTGCT | 2 |
| CACTCGGCAT | GGG | CTGAGTGGTT | 3 |
| AGCCTTGATT | ATA | CGCAAGGCTG | 3 |
| GCGTCCTTTT | GAA | TCAGGACGCT | 3 |
| GCCTTGTGAA | ATGACAGAGC | 0 | |
| GCATGCGGTT | CA | AGCCGCATGC | 2 |
DNA sequence
TGGGACTGACCCCATAATATGAGACAAATAAAAAACACCTTCTAGTTTGAAAACGGATAGTTGTTATCCGAAATAAGACTTGGAGGTGTTTTTTCTATGG
GGACAAGAGTGAGTTATCCGGTTGAAGTGAAACAGAAGGCTGTAGAAATGAGATTGGCAGGCGTGCCTATGAAAGAGATCATGCAGAAATTGAATATTAA
GAATAAGACACAGGTTCAGACATGGGTTAGATGGCATAAGGCTGGGGATACACATCGATTCGAACAGCCTGTTGGAAAGCAATACACTTACGGAAAAGGT
CCAGAGTATTCTTCCGAATTAGAGAAACTGCAGGCAGAGAATCATTATTTGAGACAACAGAATGAAGTTTTAAAAAAGTACAACGAATTGGAAAGGAAGT
TGATAGCGAAACGTCAGTCGAACTTGTAGAAGTATTGCACAGCACAATGACCGTGCAGGATATCTGTATTCATTTAGGTATCTCTCGAGCGTCTTATTAT
CGTTGGAAGAAGAATCTGACGAAAGATCATCCCAAGCGCCATTTGGAAAAACAAATCGGCACGTTGTGCCGAGAGCACAAGTATCGATATGGATATCGAA
AAATCACAGCCATACTAAAAAAGAGAATGCGTATTAACCATAAAACTGTTCAACGTATTATGCAGAAAAATCAATGGCAGTGCCAGGTTAAGGTGAAAAA
GCGCAAGAAGAATGGGCAGCCATATGCCGTGGTCGATAATATATTGGATCGGAACTTTCAGTCTGATCGCCCTCTTGAAAAACTAGTAACGGACATCACA
TATTTGCCTTATGGACAGAAACAATTGTACCTTTCCAGTATATTAGATTTATACAATGGAGAAGTGATTGCTTTTACAATTGGCGCTAAGCAGGACACAG
ACTTTGTCTTAGACACACTTAATCAGCTGCCAACACTGCCTGAGAACTGCGTGTTACATAGTGACCAAGGTTCTGTGTATACATCTTACGAGTATCAGAA
AGCTGTTAACACAAAAGGCATTACCATGAGCATGTCCCGCAAAGGGACGCCCGCTGATAATGCCTCCATCGAATCGTTTCATTCCTCACTAAAGTCTGAA
ACGTTCTATCTTAACAGCATTGATCGAACCACGACCGCCATCGTAGAACGCACTGTCATAGAATACATTCATTATTATAACAATATTCGTATTCAAACGA
AACTAAACAACCAATCACCGATAAACTATCGGCAATTGGCTGTTTAAAAGGTGTTTTGATCCCTGTCTCAAAAACGGGGGTCAGTCCCA
GGACAAGAGTGAGTTATCCGGTTGAAGTGAAACAGAAGGCTGTAGAAATGAGATTGGCAGGCGTGCCTATGAAAGAGATCATGCAGAAATTGAATATTAA
GAATAAGACACAGGTTCAGACATGGGTTAGATGGCATAAGGCTGGGGATACACATCGATTCGAACAGCCTGTTGGAAAGCAATACACTTACGGAAAAGGT
CCAGAGTATTCTTCCGAATTAGAGAAACTGCAGGCAGAGAATCATTATTTGAGACAACAGAATGAAGTTTTAAAAAAGTACAACGAATTGGAAAGGAAGT
TGATAGCGAAACGTCAGTCGAACTTGTAGAAGTATTGCACAGCACAATGACCGTGCAGGATATCTGTATTCATTTAGGTATCTCTCGAGCGTCTTATTAT
CGTTGGAAGAAGAATCTGACGAAAGATCATCCCAAGCGCCATTTGGAAAAACAAATCGGCACGTTGTGCCGAGAGCACAAGTATCGATATGGATATCGAA
AAATCACAGCCATACTAAAAAAGAGAATGCGTATTAACCATAAAACTGTTCAACGTATTATGCAGAAAAATCAATGGCAGTGCCAGGTTAAGGTGAAAAA
GCGCAAGAAGAATGGGCAGCCATATGCCGTGGTCGATAATATATTGGATCGGAACTTTCAGTCTGATCGCCCTCTTGAAAAACTAGTAACGGACATCACA
TATTTGCCTTATGGACAGAAACAATTGTACCTTTCCAGTATATTAGATTTATACAATGGAGAAGTGATTGCTTTTACAATTGGCGCTAAGCAGGACACAG
ACTTTGTCTTAGACACACTTAATCAGCTGCCAACACTGCCTGAGAACTGCGTGTTACATAGTGACCAAGGTTCTGTGTATACATCTTACGAGTATCAGAA
AGCTGTTAACACAAAAGGCATTACCATGAGCATGTCCCGCAAAGGGACGCCCGCTGATAATGCCTCCATCGAATCGTTTCATTCCTCACTAAAGTCTGAA
ACGTTCTATCTTAACAGCATTGATCGAACCACGACCGCCATCGTAGAACGCACTGTCATAGAATACATTCATTATTATAACAATATTCGTATTCAAACGA
AACTAAACAACCAATCACCGATAAACTATCGGCAATTGGCTGTTTAAAAGGTGTTTTGATCCCTGTCTCAAAAACGGGGGTCAGTCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 333 bp | 110 aa | 97 | 429 | + | No |
Description : First part of the transposase
ORF sequence :
MGTRVSYPVEVKQKAVEMRLAGVPMKEIMQKLNIKNKTQVQTWVRWHKAGDTHRFEQPVGKQYTYGKGPEYSSELEKLQAENHYLRQQNEVLKKYNELER
KLIAKRQSNL
KLIAKRQSNL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 882 bp | 293 aa | 366 | 1247 | + | No |
Description : Second part of the transposase
ORF sequence :
SFKKVQRIGKEVDSETSVELVEVLHSTMTVQDICIHLGISRASYYRWKKNLTKDHPKRHLEKQIGTLCREHKYRYGYRKITAILKKRMRINHKTVQRIMQ
KNQWQCQVKVKKRKKNGQPYAVVDNILDRNFQSDRPLEKLVTDITYLPYGQKQLYLSSILDLYNGEVIAFTIGAKQDTDFVLDTLNQLPTLPENCVLHSD
QGSVYTSYEYQKAVNTKGITMSMSRKGTPADNASIESFHSSLKSETFYLNSIDRTTTAIVERTVIEYIHYYNNIRIQTKLNNQSPINYRQLAV
KNQWQCQVKVKKRKKNGQPYAVVDNILDRNFQSDRPLEKLVTDITYLPYGQKQLYLSSILDLYNGEVIAFTIGAKQDTDFVLDTLNQLPTLPENCVLHSD
QGSVYTSYEYQKAVNTKGITMSMSRKGTPADNASIESFHSSLKSETFYLNSIDRTTTAIVERTVIEYIHYYNNIRIQTKLNNQSPINYRQLAV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 97 | 1247 | + | Yes |
Chemistry : DDE
ORF sequence :
MGTRVSYPVEVKQKAVEMRLAGVPMKEIMQKLNIKNKTQVQTWVRWHKAGDTHRFEQPVGKQYTYGKGPEYSSELEKLQAENHYLRQQNEVLKKVQRIGK
EVDSETSVELVEVLHSTMTVQDICIHLGISRASYYRWKKNLTKDHPKRHLEKQIGTLCREHKYRYGYRKITAILKKRMRINHKTVQRIMQKNQWQCQVKV
KKRKKNGQPYAVVDNILDRNFQSDRPLEKLVTDITYLPYGQKQLYLSSILDLYNGEVIAFTIGAKQDTDFVLDTLNQLPTLPENCVLHSDQGSVYTSYEY
QKAVNTKGITMSMSRKGTPADNASIESFHSSLKSETFYLNSIDRTTTAIVERTVIEYIHYYNNIRIQTKLNNQSPINYRQLAV
EVDSETSVELVEVLHSTMTVQDICIHLGISRASYYRWKKNLTKDHPKRHLEKQIGTLCREHKYRYGYRKITAILKKRMRINHKTVQRIMQKNQWQCQVKV
KKRKKNGQPYAVVDNILDRNFQSDRPLEKLVTDITYLPYGQKQLYLSSILDLYNGEVIAFTIGAKQDTDFVLDTLNQLPTLPENCVLHSDQGSVYTSYEY
QKAVNTKGITMSMSRKGTPADNASIESFHSSLKSETFYLNSIDRTTTAIVERTVIEYIHYYNNIRIQTKLNNQSPINYRQLAV
Blast result :
Comments
ISBsu1 is 71% aa similar to ISLmo1. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder (2011)
2] Zeigler,D.R. (2011) Microbiology (Reading, Engl.) 157 (PT 7), 2033-2041.
2] Zeigler,D.R. (2011) Microbiology (Reading, Engl.) 157 (PT 7), 2033-2041.