ISPa36

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AEVV01000002 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa 138244 PA13
DNA section
IS Length : 2468 bp

Ends


IR Length : 23/27

IRL : TGTTGGCGCGCATCCAGAACTGACCCACTTTCGGCGGTGATGCGCGGGTA
IRR : TGTTGGCGCGCACTTAAAACTGACCCAGGCTGCGCGTTGAACTTTGACCC

Insertion site


Left flankDirect repeatRight flankDR Length
GAAAGTGCCTGACGc/tTGGCAGCGGG7

DNA sequence

TGTTGGCGCGCATCCAGAACTGACCCACTTTCGGCGGTGATGCGCGGGTAAAACTGACCCACCTGAAGCCCACATCCTGAGCATCTTTTGCTCGGGGGAT
TTCAGGAGTGATCACCATGGTCATGTTGGCCAAGATCCGGCGGATGCATTTCCGCGATGGCCTGCCGCTGCGCGAGATCGCCAAGCGCACGGGCCTATCC
CGCAATACCATCCGGCGATGGCTGCGCAGCGGCCAGTCCGAGCCGGTCTATCCCAGGCGAGCCTCGCCCTCCCGGCTCGATCCCTACCGGTCCCAGTTGG
AGACGTGGCTGCGTGCCGACAGCCATCGTCCCCGGCGGGAACAACGCACCGCCAAGGTGCTGTTCTCGCAGTTGCAGGCGATCGGCTATCCGGGCACCTA
CACCCGGGTGACGGCCTTCATCCGCCAGTGGAAGGCGGCCGGCGGCGCCAGCCGCCGCCCGGCCTTCGTGCCGTTGCACTTTCCTCCGGGTGAAGCCTTC
CAGTTCGACTGGAGCCGCGAGTACGCCTTCGTCGGCAGCCACGCCAAGATGCCGCTGGATCTCGCCCACGTGAAGCTGGCCTGCAGCCGCGCCTTCTGGT
TGGTGGCCTATCCCACCCAGAGCCACGAGATGCTGTTCGATGCTCATACCCAGGCGTTTACCGCCTTCGGTGGCGTGCCGCGCCGCGGCATCTACGACAA
CATGAAGACGGCGGTGGACGGGATCGGCCAGCACCGGGAACGGCGGGTCAACGCCCGCTTCCACGCCATGACCAGCCACTACCTGTTCGCCGCGGAGTTC
TGCAACCGCGCCGCGGGTTGGGAGAAGGGGCGGGTCGAGAAGAACGTGCAGGACCGGCGCCGACAGATCTGGATCGAGGCCGGGGCCCGCCACTGGCCAG
ATCTGGACACCCTCAATGCCTGGCTCGCCAGCGAGTGCCGCGCCGCTTGGCCGATGCAGGCGCATCCGGATGCCTCCGACATCACCGTGGCCGAAGCTCT
GGAGGACGAACAGCCCCACCTGATGCCGATGCCCGCGCCCTTCGACGGCTACATCGAGCGGCCGGTGCGGGTCAGTTCGACCGCGCTGGTCAGCTTCCAG
CGCAACCGCTACAGCGTGCCGTGCGAACACGCCCACGCCGTCGCCAGCCTGCGCGTGTATCCCTTCGAACTGGCCGTGGTGGTCGGCGAGGCCGAGGTCG
CCCGCCACCGGCGCAGCTTCGAGCGTGATCGGGTCCACTACGACTGGCAGCACTACATCGCCCTGGTCCAGCGCAAGCCGGGGGCACTGCGTGACGGCGC
GCCGTTCCTGTCCATGCCCGATCCGCTGCGCAAGCTGCAGCAGGTGTTGCTGCGCCAGCCTCACGGCGACCGCGCCATGGCCCAGGTGCTGGCCGCCGTA
CCCGTGCACGGGCTCGAAGCGGTACTGGTGGCGGTGGAACTGGCACTCGAGTCCGGCCTGGTGCGCGCCGACCACGTGCTCAACGTGCTGGGGCGATTGC
AGCATCCGGACCGCACGATCGAGACGGTCGCCTCGCGAGTGACGCTGCGTCATCCGCCGGTGTCCGATCCGGCCCGCTACGACGCCCTGCGGGAGGCAGA
TCATGTCGACGCCTGACCTGCCGCTGCGCATGCAGCAGCTCAAGCTGCACGGCATGGCCAGCCAGTGGCCCGAACTGCTGGCCAAGATGCGCCTGCACAC
CATGGAGCCCGAACACTGGATGGCCGAACTGCTGCAGGCCGAGTCCGCCGAACGGCAGGTTCGTGCCCAGGCCAACCAGATGAAGGCCGCACGTTTCCCC
GTCCATCGTGACCTGCTCGGGTTCGACTTCGCGGCCAGTCCCGTTGATCGCGCACTGGTCGAACGACTCCACCAGGGTGACTTCATCCACACCCCCGAGA
ACGTCGTCCTTGTCGGTGGACCGGGCACCGGCAAGACCCACCTGGCCACCGCCATCGGCATCCAGGCCGTGCGCCAGCATCGCCTGCGCGTGCGCTTCTT
CTCCACCATCGAACTGGTCAATGCGCTGGAGAAGGAACAGGCCCAGGGCAAGGCCGGGCAGATCGCCCTGCGCCTGACCTATGCCGACCTGGTCATCCTC
GATGAACTGGGCTACCTGCCGTTCACCCCCTCAGGCGGCGCGCTGCTGTTCCACCTGATCAGCAAGCTCTACGAGCGCACCAGCCTCGCCATCACCACCA
ACTTGGCCTTCTCCGAATGGGGTCAGGTGTTCGGCGACGCCAAGATGACCACCGCGCTGCTGGATCGCATCACCCACCACTGCCACATCCTGGAGACCGG
CAACGACTCCTGGCGGCTGCGCAACAGCACCGCCGCCAACAAGACCAAAGCCAGAATCAGGGCAAAGGAGGCCACCACCCAGACCACCAACTGATACCCT
CAGCCCCGTACACCGGGTGGGTCAAAGTTCAACGCGCAGCCTGGGTCAGTTTTAAGTGCGCGCCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1500 bp499 aa1171616+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVMLAKIRRMHFRDGLPLREIAKRTGLSRNTIRRWLRSGQSEPVYPRRASPSRLDPYRSQLETWLRADSHRPRREQRTAKVLFSQLQAIGYPGTYTRVTA
FIRQWKAAGGASRRPAFVPLHFPPGEAFQFDWSREYAFVGSHAKMPLDLAHVKLACSRAFWLVAYPTQSHEMLFDAHTQAFTAFGGVPRRGIYDNMKTAV
DGIGQHRERRVNARFHAMTSHYLFAAEFCNRAAGWEKGRVEKNVQDRRRQIWIEAGARHWPDLDTLNAWLASECRAAWPMQAHPDASDITVAEALEDEQP
HLMPMPAPFDGYIERPVRVSSTALVSFQRNRYSVPCEHAHAVASLRVYPFELAVVVGEAEVARHRRSFERDRVHYDWQHYIALVQRKPGALRDGAPFLSM
PDPLRKLQQVLLRQPHGDRAMAQVLAAVPVHGLEAVLVAVELALESGLVRADHVLNVLGRLQHPDRTIETVASRVTLRHPPVSDPARYDALREADHVDA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
792 bp263 aa16032394+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSTPDLPLRMQQLKLHGMASQWPELLAKMRLHTMEPEHWMAELLQAESAERQVRAQANQMKAARFPVHRDLLGFDFAASPVDRALVERLHQGDFIHTPEN
VVLVGGPGTGKTHLATAIGIQAVRQHRLRVRFFSTIELVNALEKEQAQGKAGQIALRLTYADLVILDELGYLPFTPSGGALLFHLISKLYERTSLAITTN
LAFSEWGQVFGDAKMTTALLDRITHHCHILETGNDSWRLRNSTAANKTKARIRAKEATTQTTN

 

Blast result :
Comments
ISPa36 is 71% (istA, the Transposase) and 77% (istB) aa similar to ISAav1.
References
1] ISfinder annotation (2011).
2] Soares-Castro,P., Marques,D., Demyanchuk,S., Faustino,A. and Santos,P.M. (2011) J. Bacteriol. 193 (19), 5573.
3] Martin Holm Rau (2011) Direct submission to ISfinder.