ISAba20
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter baumannii | Acinetobacter baumannii WH8144 plasmid pWH8144 |
DNA section
IS Length : 1199 bp
Ends
IR Length : 33/46
IRL : GGTAATCCTCCCTAAAAATAATTGAGTTTAAAAGTAGAAAATCGAATAAC
IRR : GGTAATACCCCCATTTTTAATTGAATTAATGGGTAGAAAATCTAAAGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTTATTTAC | TAAG | TTTTCTAATA | 4 |
DNA sequence
GGTAATCCTCCCTAAAAATAATTGAGTTTAAAAGTAGAAAATCGAATAACCTTGATTTAAGGAGATTTTCTATGAAAACATCTAAGTATTCAGACAGCTT
GATCATGAGTATCTTGAAGCAAGCTGAAGCAGGAACACCCATACCGAACTTGTGCCGCGAACATGGTATGAGCTCTGCTACATTCTACAAGTGGAAGTCC
AAATATGGCGGCATGGATCTTTCAATGATGACGCGGCTTAAAGAACTTGAGGCTGAAAATGCCCGTTTAAAACGCATGTATGCTGATGAGCGGCTAAAGT
CAGACATCTTGCAAGATGCACTTTTAAAAAAGTTTTAAGGCCTGTTCAGCGAAAGTCACTTGCTCAGCAAGCTACTGAGCAATTTAAGATTTCAATCAGC
ATGGCCTGCTTAATTTTTAAAGTCAGTCAAACGTGTTATCGCTATAAAGCTCGTTTAGATGAAGAGAATCAACAGATTGCCGAATGGTTGATTCAATTAA
CCAATGATCATAAAACATGGGGCTTCAAGCTTTGTTTCCTGTATTTGCGTAATGTTAAAAAATACAAATGGAACCACAAACGTGTCTATCGCATTTATAA
GGAGCTTGAGCTTAATTTAAGAATCAAGCCTAATAAGCGGATTAAACGAGAACAGCCAGAAACCCTTGCAGTACCGACAGCTAAGAATGAATCATGGTCA
ATGGACTTTATGCATGATCAACTCAGTGATGGACGTAGTTATCGAGTTCACAACGTGATTGATGACTATAATCGCGAGTCATTGGATATATTGATTGACT
TTTCATTGCCAGCAGAACGTGTACTGAGAGGACTTGATCAGCTCATTGAATGGCGAGGCAAACCTAAACGCATTAGAACCGACAACGGCCCAGAGTATAT
AAGTGAGCTGATGGAAAAGTGGTGTGATCAGCATGGTATTGAGCATGTATTTACTCAACCAGGCAATCCACAGCAAAATGCATATGTTGAGCGATTTAAT
CGTACAGTACGTTATGAGTGTCTAAATCAATATTTATTTCGAGATCTGAGCGAGGTGCAGGACTATACAACTGACTGGCAGTACTTTTATAATCATGAAC
GACCTCATATGTCAGTAAATGGTTGTCCACCTATTTTTAAACAATAACAAGGCTTTAGATTTTCTACCCATTAATTCAATTAAAAATGGGGGTATTACC
GATCATGAGTATCTTGAAGCAAGCTGAAGCAGGAACACCCATACCGAACTTGTGCCGCGAACATGGTATGAGCTCTGCTACATTCTACAAGTGGAAGTCC
AAATATGGCGGCATGGATCTTTCAATGATGACGCGGCTTAAAGAACTTGAGGCTGAAAATGCCCGTTTAAAACGCATGTATGCTGATGAGCGGCTAAAGT
CAGACATCTTGCAAGATGCACTTTTAAAAAAGTTTTAAGGCCTGTTCAGCGAAAGTCACTTGCTCAGCAAGCTACTGAGCAATTTAAGATTTCAATCAGC
ATGGCCTGCTTAATTTTTAAAGTCAGTCAAACGTGTTATCGCTATAAAGCTCGTTTAGATGAAGAGAATCAACAGATTGCCGAATGGTTGATTCAATTAA
CCAATGATCATAAAACATGGGGCTTCAAGCTTTGTTTCCTGTATTTGCGTAATGTTAAAAAATACAAATGGAACCACAAACGTGTCTATCGCATTTATAA
GGAGCTTGAGCTTAATTTAAGAATCAAGCCTAATAAGCGGATTAAACGAGAACAGCCAGAAACCCTTGCAGTACCGACAGCTAAGAATGAATCATGGTCA
ATGGACTTTATGCATGATCAACTCAGTGATGGACGTAGTTATCGAGTTCACAACGTGATTGATGACTATAATCGCGAGTCATTGGATATATTGATTGACT
TTTCATTGCCAGCAGAACGTGTACTGAGAGGACTTGATCAGCTCATTGAATGGCGAGGCAAACCTAAACGCATTAGAACCGACAACGGCCCAGAGTATAT
AAGTGAGCTGATGGAAAAGTGGTGTGATCAGCATGGTATTGAGCATGTATTTACTCAACCAGGCAATCCACAGCAAAATGCATATGTTGAGCGATTTAAT
CGTACAGTACGTTATGAGTGTCTAAATCAATATTTATTTCGAGATCTGAGCGAGGTGCAGGACTATACAACTGACTGGCAGTACTTTTATAATCATGAAC
GACCTCATATGTCAGTAAATGGTTGTCCACCTATTTTTAAACAATAACAAGGCTTTAGATTTTCTACCCATTAATTCAATTAAAAATGGGGGTATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKYSDSLIMSILKQAEAGTPIPNLCREHGMSSATFYKWKSKYGGMDLSMMTRLKELEAENARLKRMYADERLKSDILQDALLKKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 858 bp | 285 aa | 290 | 1147 | + | No |
Description : Second part of the transposase
ORF sequence :
AAKVRHLARCTFKKVLRPVQRKSLAQQATEQFKISISMACLIFKVSQTCYRYKARLDEENQQIAEWLIQLTNDHKTWGFKLCFLYLRNVKKYKWNHKRVY
RIYKELELNLRIKPNKRIKREQPETLAVPTAKNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRTDNG
PEYISELMEKWCDQHGIEHVFTQPGNPQQNAYVERFNRTVRYECLNQYLFRDLSEVQDYTTDWQYFYNHERPHMSVNGCPPIFKQ
RIYKELELNLRIKPNKRIKREQPETLAVPTAKNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRTDNG
PEYISELMEKWCDQHGIEHVFTQPGNPQQNAYVERFNRTVRYECLNQYLFRDLSEVQDYTTDWQYFYNHERPHMSVNGCPPIFKQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1076 bp | 358 aa | 72 | 1147 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKYSDSLIMSILKQAEAGTPIPNLCREHGMSSATFYKWKSKYGGMDLSMMTRLKELEAENARLKRMYADERLKSDILQDALLKKVLRPVQRKSLAQQ
ATEQFKISISMACLIFKVSQTCYRYKARLDEENQQIAEWLIQLTNDHKTWGFKLCFLYLRNVKKYKWNHKRVYRIYKELELNLRIKPNKRIKREQPETLA
VPTAKNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRTDNGPEYISELMEKWCDQHGIEHVFTQPGNP
QQNAYVERFNRTVRYECLNQYLFRDLSEVQDYTTDWQYFYNHERPHMSVNGCPPIFKQ
ATEQFKISISMACLIFKVSQTCYRYKARLDEENQQIAEWLIQLTNDHKTWGFKLCFLYLRNVKKYKWNHKRVYRIYKELELNLRIKPNKRIKREQPETLA
VPTAKNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRTDNGPEYISELMEKWCDQHGIEHVFTQPGNP
QQNAYVERFNRTVRYECLNQYLFRDLSEVQDYTTDWQYFYNHERPHMSVNGCPPIFKQ
Blast result :
Comments
ISAba20 ORFA is 76% aa simlar to ISSod2 and ISShfr8, ORFB is 64% aa simlar to ISShfr8, 61% to ISKpn10 and 57% to ISSod2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAba20 orfAB is 85% aa similar to ISShfr8.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAba20 orfAB is 85% aa similar to ISShfr8.
References
1] Yiqi Fu (2011) Direct submission