ISAba19
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter baumannii | Acinetobacter baumannii |
DNA section
IS Length : 1309 bp
Ends
IR Length : 25
IRL : TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTTATTTAAGTTAAGCCACCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTATGAAAG | CTT | TGAACCGTAC | 3 |
| GTACGGTTCA | CTT | CCGCTATTCC | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCGATGACAAAACCAAACTATACCCCCGAAATTAGAGAAAGAGCGGTTCAATT
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCAGCAATCACAGCTATTGCTCCTAAAATCGGTTGTACTCCTGAAACATTGCGTGTTTGGTAT
TTAAAGCATCTGGATCAACTAAATCCTGCCAAAGTACAACAGATATCTGACCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAACGTG
CCAATGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCATAACAATAAAGATCGA
TATGGTGTTGAAGCGATTTGTAGAATTTTACCGATTGCACCTTCAACCTATTACCGAACTTTAGATCTCACTGACAATCCAGAACATCGAGCGAAACGAG
ATCTACATGATGAGTATCATGCTGAACAAATTAAACGAATTTGGAAAGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAAAAATTGAAACGTGA
GGGTTATGTTATTGCACGTTGTACAGTTGCTCGATTGATGCAAAAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACCACCCGTAACCGA
GATGACCAAAAACGGGCAGATGATTTAGTGAAACGTAATTTTAATGCTGATCATCCAAACCAACTATGGGTGGGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTATATACCGCATTTGTTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTTGATGC
ATTAGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGACAGAGGTGTGCAATATCTTTCCATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACAACGGGTGATTCATACGATAATGCTCTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAAAGCGTGTACACAGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCTTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCAGCAATCACAGCTATTGCTCCTAAAATCGGTTGTACTCCTGAAACATTGCGTGTTTGGTAT
TTAAAGCATCTGGATCAACTAAATCCTGCCAAAGTACAACAGATATCTGACCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAACGTG
CCAATGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCATAACAATAAAGATCGA
TATGGTGTTGAAGCGATTTGTAGAATTTTACCGATTGCACCTTCAACCTATTACCGAACTTTAGATCTCACTGACAATCCAGAACATCGAGCGAAACGAG
ATCTACATGATGAGTATCATGCTGAACAAATTAAACGAATTTGGAAAGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAAAAATTGAAACGTGA
GGGTTATGTTATTGCACGTTGTACAGTTGCTCGATTGATGCAAAAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACCACCCGTAACCGA
GATGACCAAAAACGGGCAGATGATTTAGTGAAACGTAATTTTAATGCTGATCATCCAAACCAACTATGGGTGGGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTATATACCGCATTTGTTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTTGATGC
ATTAGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGACAGAGGTGTGCAATATCTTTCCATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACAACGGGTGATTCATACGATAATGCTCTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAAAGCGTGTACACAGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCTTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKPNYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRVWYLKHLDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 308 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
NSTQSSRFFRPGGARPPTQIMVDFIHNNKDRYGVEAICRILPIAPSTYYRTLDLTDNPEHRAKRDLHDEYHAEQIKRIWKESSGRYGVRKVWQKLKREGY
VIARCTVARLMQKLGIQGVWRGKNKQTTRNRDDQKRADDLVKRNFNADHPNQLWVGDFTYIQTHSGWVYTAFVIDVFSRAIVGWKVSTRMNTDMVLDALE
QALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYV
SPFEFEAMYYDKINPLGQVA
VIARCTVARLMQKLGIQGVWRGKNKQTTRNRDDQKRADDLVKRNFNADHPNQLWVGDFTYIQTHSGWVYTAFVIDVFSRAIVGWKVSTRMNTDMVLDALE
QALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYV
SPFEFEAMYYDKINPLGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKPNYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRVWYLKHLDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFRPGGA
RPPTQIMVDFIHNNKDRYGVEAICRILPIAPSTYYRTLDLTDNPEHRAKRDLHDEYHAEQIKRIWKESSGRYGVRKVWQKLKREGYVIARCTVARLMQKL
GIQGVWRGKNKQTTRNRDDQKRADDLVKRNFNADHPNQLWVGDFTYIQTHSGWVYTAFVIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA
RPPTQIMVDFIHNNKDRYGVEAICRILPIAPSTYYRTLDLTDNPEHRAKRDLHDEYHAEQIKRIWKESSGRYGVRKVWQKLKREGYVIARCTVARLMQKL
GIQGVWRGKNKQTTRNRDDQKRADDLVKRNFNADHPNQLWVGDFTYIQTHSGWVYTAFVIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA
Blast result :
Comments
ISAba19 disrupting blaOXA-78.
ISAba19 is 97% aa similar to ISAba2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAba19 is 97% aa similar to ISAba2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Higgins P. (2011) Direct submission