ISWpi14
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Wolbachia pipientis | Wolbachia endosymbiont of Drosophila melanogaster Wolbachia pipientis |
DNA section
IS Length : 1288 bp
Ends
IR Length : 9/11
IRL : TGGTATGAATAGTTATTGCTTGGTTGGAAGCATGAGAAGATAATACCTTC
IRR : TCCTATGATTGTTGTCAATAAGAAATTTCATATTTTGTAAAATCTTTGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTAAGGAGA | GAAACATTTA | 0 |
DNA sequence
TGGTATGAATAGTTATTGCTTGGTTGGAAGCATGAGAAGATAATACCTTCTCAGAGTGGATAGGTGGCAGATTGAAAATGTTTGTGAGGCCCTTTCTGCT
TTTTGCAGTATAGGAAAAAGACCTCGTGAAGGAGATTAATCGCCCCTGCCACCGAAAACCATCGTGCGGCCTATAAAATTTAGTGACCGCGTATAGGAGA
ATGGACCAACCGGTAATATTCTACTCTGATATGTTGGAGTATTCAATAAGGGAGAAGATTTATGCGTTACATAGGTGTTGATTTACATACTAATAGTTTC
ACTGCTTGCTACTTAGAACAGGAAAAACCAGAGTATATTAAAACGTTTCGCTTACAGGATTTAGGTAATTTTATCAAAAATCTTCAAGAAACAGATGAAG
TAGCCTTAGAGGCAACGGGAAATAGCTGTTTCTTTTATGATGAAGTGTCACCTTACGTTAAGCGCGTTGTTGTGGTTGCTCCCTTGCAATTTGAAGTTAT
TCGTCGTTCTGTGAACAAAACAGATAAACATGATGCAAGAGCTATTGCTTTTTTTCTCAGCAAAGATATGTTGCCTGAAGCAAAATGCAAGAGCAAACAA
TGCCAACAGTTAGCTTCTCTTCTTAAAACAAGAGATCAGTTAGTAAAATCACGGGTATCTTTAATCAATAAAATACATGGACTTTTCAATTACCACGGAA
TAAAGATTAAAAAGGAAGTACTGACAATCAAAGCGGGATTTGAATGCTCTCTTGGTAAACATGATTGGAGTCACTTGGAAAAGGTTGAGATTGAAGTGAT
TAGCAGTAATCTGGAAGTCATTCGAGAAAGTCTCAAAAAACTTGAAAAGGAAATTATAGCTTTTGCTCAGCAGCTTCCTGGATTTAATAATCTCATCAGC
ATTAAAGGTATTGGTGCGATTTCTGCAGCAGTTTTTGTTGCAACAATTGGTGATATTAATGATTTTAGTAATCCTGAAAAACTTACAGCTTATTTTGGAG
TTGTACCGCGAGTTTCTCAATCCAATCAACAATGCACAATTGGAAGAATTACTAAACGTGGATCAAAAATTGGGCGTACTTCTTTAGTACAGTGTACTTG
GATTGCTATACGTTATAGTCCTTATCTGAAAAGTTTTTACGAGCGTGTGAAAAAAAGCGTGGTTCTGCTAAGGCTATTATTGCTACTGCTAGGAAGTTTC
TCACAACTATTTTCTATACTCTTAAAAACAACTGGATTTTCAAAGATTTTACAAAATATGAAATTTCTTATTGACAACAATCATAGGA
TTTTGCAGTATAGGAAAAAGACCTCGTGAAGGAGATTAATCGCCCCTGCCACCGAAAACCATCGTGCGGCCTATAAAATTTAGTGACCGCGTATAGGAGA
ATGGACCAACCGGTAATATTCTACTCTGATATGTTGGAGTATTCAATAAGGGAGAAGATTTATGCGTTACATAGGTGTTGATTTACATACTAATAGTTTC
ACTGCTTGCTACTTAGAACAGGAAAAACCAGAGTATATTAAAACGTTTCGCTTACAGGATTTAGGTAATTTTATCAAAAATCTTCAAGAAACAGATGAAG
TAGCCTTAGAGGCAACGGGAAATAGCTGTTTCTTTTATGATGAAGTGTCACCTTACGTTAAGCGCGTTGTTGTGGTTGCTCCCTTGCAATTTGAAGTTAT
TCGTCGTTCTGTGAACAAAACAGATAAACATGATGCAAGAGCTATTGCTTTTTTTCTCAGCAAAGATATGTTGCCTGAAGCAAAATGCAAGAGCAAACAA
TGCCAACAGTTAGCTTCTCTTCTTAAAACAAGAGATCAGTTAGTAAAATCACGGGTATCTTTAATCAATAAAATACATGGACTTTTCAATTACCACGGAA
TAAAGATTAAAAAGGAAGTACTGACAATCAAAGCGGGATTTGAATGCTCTCTTGGTAAACATGATTGGAGTCACTTGGAAAAGGTTGAGATTGAAGTGAT
TAGCAGTAATCTGGAAGTCATTCGAGAAAGTCTCAAAAAACTTGAAAAGGAAATTATAGCTTTTGCTCAGCAGCTTCCTGGATTTAATAATCTCATCAGC
ATTAAAGGTATTGGTGCGATTTCTGCAGCAGTTTTTGTTGCAACAATTGGTGATATTAATGATTTTAGTAATCCTGAAAAACTTACAGCTTATTTTGGAG
TTGTACCGCGAGTTTCTCAATCCAATCAACAATGCACAATTGGAAGAATTACTAAACGTGGATCAAAAATTGGGCGTACTTCTTTAGTACAGTGTACTTG
GATTGCTATACGTTATAGTCCTTATCTGAAAAGTTTTTACGAGCGTGTGAAAAAAAGCGTGGTTCTGCTAAGGCTATTATTGCTACTGCTAGGAAGTTTC
TCACAACTATTTTCTATACTCTTAAAAACAACTGGATTTTCAAAGATTTTACAAAATATGAAATTTCTTATTGACAACAATCATAGGA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1062 bp | 353 aa | 362 | 1423 | + | No |
Description : First part of the transposase
ORF sequence :
MRYIGVDLHTNSFTACYLEQEKPEYIKTFRLQDLGNFIKNLQETDEVALEATGNSCFFYDEVSPYVKRVVVVAPLQFEVIRRSVNKTDKHDARAIAFFLS
KDMLPEAKCKSKQCQQLASLLKTRDQLVKSRVSLINKIHGLFNYHGIKIKKEVLTIKAGFECSLGKHDWSHLEKVEIEVISSNLEVIRESLKKLEKEIIA
FAQQLPGFNNLISIKGIGAISAAVFVATIGDINDFSNPEKLTAYFGVVPRVSQSNQQCTIGRITKRGSKIGRTSLVQCTWIAIRYSPYLKSFYERVKKSV
VLLRLLLLLLGSFSQLFSILLKTTGFSKILQNMKFLIDNNHRRNIYELYNRTK
KDMLPEAKCKSKQCQQLASLLKTRDQLVKSRVSLINKIHGLFNYHGIKIKKEVLTIKAGFECSLGKHDWSHLEKVEIEVISSNLEVIRESLKKLEKEIIA
FAQQLPGFNNLISIKGIGAISAAVFVATIGDINDFSNPEKLTAYFGVVPRVSQSNQQCTIGRITKRGSKIGRTSLVQCTWIAIRYSPYLKSFYERVKKSV
VLLRLLLLLLGSFSQLFSILLKTTGFSKILQNMKFLIDNNHRRNIYELYNRTK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 156 bp | 51 aa | 1219 | 1374 | + | No |
Description : Second part of the transposase
ORF sequence :
SLSEKFLRACEKKRGSAKAIIATARKFLTTIFYTLKNNWIFKDFTKYEISY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1013 bp | 337 aa | 362 | 1374 | + | Yes |
Chemistry : DEDD
ORF sequence :
MRYIGVDLHTNSFTACYLEQEKPEYIKTFRLQDLGNFIKNLQETDEVALEATGNSCFFYDEVSPYVKRVVVVAPLQFEVIRRSVNKTDKHDARAIAFFLS
KDMLPEAKCKSKQCQQLASLLKTRDQLVKSRVSLINKIHGLFNYHGIKIKKEVLTIKAGFECSLGKHDWSHLEKVEIEVISSNLEVIRESLKKLEKEIIA
FAQQLPGFNNLISIKGIGAISAAVFVATIGDINDFSNPEKLTAYFGVVPRVSQSNQQCTIGRITKRGSKIGRTSLVQCTWIAIRYSPYLKSFYERVKKKR
GSAKAIIATARKFLTTIFYTLKNNWIFKDFTKYEISY
KDMLPEAKCKSKQCQQLASLLKTRDQLVKSRVSLINKIHGLFNYHGIKIKKEVLTIKAGFECSLGKHDWSHLEKVEIEVISSNLEVIRESLKKLEKEIIA
FAQQLPGFNNLISIKGIGAISAAVFVATIGDINDFSNPEKLTAYFGVVPRVSQSNQQCTIGRITKRGSKIGRTSLVQCTWIAIRYSPYLKSFYERVKKKR
GSAKAIIATARKFLTTIFYTLKNNWIFKDFTKYEISY
Blast result :
Comments
Putative boundaries identified by aligning IS flanks with an empty insertion site.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISWpi14 is 52% aa similar to ISAtu7.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISWpi14 is 52% aa similar to ISAtu7.
References
1] Cerveau N., and Cordaux R. (2010) Direct submission (manuscript in preparation)