ISAba18
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000521 | ND | Acinetobacter baumannii | Acinetobacter baumannii ATCC 17978 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 24/25
IRL : TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATTCCG
IRR : TGAACCGTACCGAGTTTGTCGGAGACTTTTTTATTTAAGTTAGGCCACCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTACAGACC | ACA | CCATACTCGG | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATTCCGATGACAAAATTAAAATATACCCCTGAAATCAGAGAAAGAGCGGTTCAACT
TGTGATTGAATCCGAAAAAGATTATCCATCGAACTGGGCAGCAGTTTCCGCAATTGCTCCTAAAATTGGTTGTACTCCTGAAACGCTGCGTGTTTGGTAT
CAAAAATATTTAGATCAACAAAACCCAATCAAAGTACAACAATTTTCAGATCAAGAGCGTATTAAACAACTCGAACGTGAAAATAAAGAACTGCAACGCG
CTAACGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCATACAAATAATGGTGGATTTCATTCATAACAATAAAGCGTTA
TACGGTGTTGATGCGATTTGTAGAATATTACCCATCGCAGCATCAACCTATTACCGGACTTTAGATCTCGTTAATAATCCAGAATTTCGAGCGAAACGTG
CTCTGCATGATTTACATCATGCAGAGCAAATTAAACGTATTTGGAAGGAAAGTTTAGGCCGATATGGCATACGTAAAGTATGGCAACAACTGAGACGTGA
AGGCTATGTGATCGCACGTTGCACAGTAGTTCGCTTGATGCGAAAGCTAAACATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAACGAGCAGATGATTTAGTGAAACGTAATTTTAAAGCTGATCACCCTAATCACTTGTGGGTTGCTGACTTCACCTATATTCAAACCAATT
CAGGCTGGGTGTATACCGCATTTATTATTGATGTGTTCTCACGAGCAATTGTTGGGTGGAAGGTATCGACACGCATGAATACGGATATGGTGCTCGATGC
ATTGGAGCAAGCATTGCATGATCGAGGTATGCCAAAGAATGTTATTCATCATTCTGACAGAGGGGTTCAATATCTCTCCATTCGCTATACTAATCGTTTA
GAAGCAGCAAATTTAAGAGCATCAGTCGGTACAACTGGTGATTCTTACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACTGAGGTGATTG
AATATTTAAAAGCAGATTGGCAGGGTTTAGCTGATGTACAACTTGCCACATTGAATTGGGTGGATTGGTTCAATAAAAAGCGTATACACAGTGCACTTGG
TTATGTATCACCTTTTGATTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGACAAACTCGG
TACGGTTCA
TGTGATTGAATCCGAAAAAGATTATCCATCGAACTGGGCAGCAGTTTCCGCAATTGCTCCTAAAATTGGTTGTACTCCTGAAACGCTGCGTGTTTGGTAT
CAAAAATATTTAGATCAACAAAACCCAATCAAAGTACAACAATTTTCAGATCAAGAGCGTATTAAACAACTCGAACGTGAAAATAAAGAACTGCAACGCG
CTAACGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCATACAAATAATGGTGGATTTCATTCATAACAATAAAGCGTTA
TACGGTGTTGATGCGATTTGTAGAATATTACCCATCGCAGCATCAACCTATTACCGGACTTTAGATCTCGTTAATAATCCAGAATTTCGAGCGAAACGTG
CTCTGCATGATTTACATCATGCAGAGCAAATTAAACGTATTTGGAAGGAAAGTTTAGGCCGATATGGCATACGTAAAGTATGGCAACAACTGAGACGTGA
AGGCTATGTGATCGCACGTTGCACAGTAGTTCGCTTGATGCGAAAGCTAAACATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAACGAGCAGATGATTTAGTGAAACGTAATTTTAAAGCTGATCACCCTAATCACTTGTGGGTTGCTGACTTCACCTATATTCAAACCAATT
CAGGCTGGGTGTATACCGCATTTATTATTGATGTGTTCTCACGAGCAATTGTTGGGTGGAAGGTATCGACACGCATGAATACGGATATGGTGCTCGATGC
ATTGGAGCAAGCATTGCATGATCGAGGTATGCCAAAGAATGTTATTCATCATTCTGACAGAGGGGTTCAATATCTCTCCATTCGCTATACTAATCGTTTA
GAAGCAGCAAATTTAAGAGCATCAGTCGGTACAACTGGTGATTCTTACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACTGAGGTGATTG
AATATTTAAAAGCAGATTGGCAGGGTTTAGCTGATGTACAACTTGCCACATTGAATTGGGTGGATTGGTTCAATAAAAAGCGTATACACAGTGCACTTGG
TTATGTATCACCTTTTGATTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGACAAACTCGG
TACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKLKYTPEIRERAVQLVIESEKDYPSNWAAVSAIAPKIGCTPETLRVWYQKYLDQQNPIKVQQFSDQERIKQLERENKELQRANEILRKAAAFFAQAEL
DRPYK
DRPYK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 365 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
RNSTQSSRFFRPGGARPPIQIMVDFIHNNKALYGVDAICRILPIAASTYYRTLDLVNNPEFRAKRALHDLHHAEQIKRIWKESLGRYGIRKVWQQLRREG
YVIARCTVVRLMRKLNIQGVWRGKNKQTTRSRDDQKRADDLVKRNFKADHPNHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDAL
EQALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRIHSALGY
VSPFDFEAMYYDKINPLGQVA
YVIARCTVVRLMRKLNIQGVWRGKNKQTTRSRDDQKRADDLVKRNFKADHPNHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDAL
EQALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRIHSALGY
VSPFDFEAMYYDKINPLGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKLKYTPEIRERAVQLVIESEKDYPSNWAAVSAIAPKIGCTPETLRVWYQKYLDQQNPIKVQQFSDQERIKQLERENKELQRANEILRKAAAFFRPGGA
RPPIQIMVDFIHNNKALYGVDAICRILPIAASTYYRTLDLVNNPEFRAKRALHDLHHAEQIKRIWKESLGRYGIRKVWQQLRREGYVIARCTVVRLMRKL
NIQGVWRGKNKQTTRSRDDQKRADDLVKRNFKADHPNHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRIHSALGYVSPFDFEAMYYDKIN
PLGQVA
RPPIQIMVDFIHNNKALYGVDAICRILPIAASTYYRTLDLVNNPEFRAKRALHDLHHAEQIKRIWKESLGRYGIRKVWQQLRREGYVIARCTVVRLMRKL
NIQGVWRGKNKQTTRSRDDQKRADDLVKRNFKADHPNHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRIHSALGYVSPFDFEAMYYDKIN
PLGQVA
Blast result :
Comments
ISAba18 is 88% identical to ISAba2. In [1] it is referred to as ISAba2-like.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Nigro, S.J. and Hall R.M. (2011) J. Antimicrob. Chemother. 66 (9), 2175-2176
2] Smith,M.G., Gianoulis,T.A., Pukatzki,S., Mekalanos,J.J., Ornston,L.N., Gerstein,M. and Snyder,M. (2007) Genes Dev. 21 (5), 601-614
2] Smith,M.G., Gianoulis,T.A., Pukatzki,S., Mekalanos,J.J., Ornston,L.N., Gerstein,M. and Snyder,M. (2007) Genes Dev. 21 (5), 601-614