ISAba15
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JN187417 | ND | Acinetobacter baumannii | Acinetobacter baumannii B0707-070 |
DNA section
IS Length : 873 bp
Ends
IR Length : 14/15
IRL : GAACCTGTTCACGATTAAAAAATATGCTAGGATAAGCCACATAATCGATT
IRR : GAACCTGTTCACAATCTTTTCAGCAACAAAGCGATGAAAGCAAGATTGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GAACCTGTTCACGATTAAAAAATATGCTAGGATAAGCCACATAATCGATTAATAACCGAATAATTCACCATGAGAAAAGCCTATCCAAGCGACATTAGCC
GAAAACAATTTGAGCACATACTACCCATTCTAGAAAGTGCAAGGAAAAAGACCAAACCAAGAACGGTTGACCTTTATGACGTATTTTGTGGGTTATTGTA
TGTACTAAGAACAGGTTGCCAATGGCGACAGCTGCCCCATGACTTTCCCAAATGGCGCACCGTCCACTCGTACTTTCAGAAATGGAGCGAGCTTGACGAA
GAAGGCAACAGCATTCTTGACCAAGCCTTAAAAAAAATTGGTCAAAAAAGCGCGAAAGAAGGACAAACGTAAGAGCAAAACCAGTTTTATCATCATTGAT
GCTCAAAGCGTAAAAAATACCGACACGGCTAAACACAAAGGCTATGACGCAGGAAAGAAAGTATCAGGGATTAAGCGCCATATTGCGGTAGATAGCCAAG
GTTTGCCGCATGCTATCTGTATTACCACTGCCAATATCACCGACCGTGAGGGTGCTAAAACGTTACTTCGTCAAAATACCAAACGATTAAGCCGCGTAAA
AAATGTGATGGTTGATGGCGGTTATCGAGGTGAGGCATTTGCCAATAGTGTAAAGACCCTTTTAGGCGAAACCGTTACCACAGAAGTCGCCAAAAGAGAT
GAACTGCATACGTTTAAAGTCATACCAAAGCGATGGGTTGTAGAACGTTCGTTTGCTTGGCTTGAGAAAAATAGACGTCTATGGAAAAACTGTGAGCGTC
TTTTAAATTCGAGCCTTCAATTTACCAATCTTGCTTTCATCGCTTTGTTGCTGAAAAGATTGTGAACAGGTTC
GAAAACAATTTGAGCACATACTACCCATTCTAGAAAGTGCAAGGAAAAAGACCAAACCAAGAACGGTTGACCTTTATGACGTATTTTGTGGGTTATTGTA
TGTACTAAGAACAGGTTGCCAATGGCGACAGCTGCCCCATGACTTTCCCAAATGGCGCACCGTCCACTCGTACTTTCAGAAATGGAGCGAGCTTGACGAA
GAAGGCAACAGCATTCTTGACCAAGCCTTAAAAAAAATTGGTCAAAAAAGCGCGAAAGAAGGACAAACGTAAGAGCAAAACCAGTTTTATCATCATTGAT
GCTCAAAGCGTAAAAAATACCGACACGGCTAAACACAAAGGCTATGACGCAGGAAAGAAAGTATCAGGGATTAAGCGCCATATTGCGGTAGATAGCCAAG
GTTTGCCGCATGCTATCTGTATTACCACTGCCAATATCACCGACCGTGAGGGTGCTAAAACGTTACTTCGTCAAAATACCAAACGATTAAGCCGCGTAAA
AAATGTGATGGTTGATGGCGGTTATCGAGGTGAGGCATTTGCCAATAGTGTAAAGACCCTTTTAGGCGAAACCGTTACCACAGAAGTCGCCAAAAGAGAT
GAACTGCATACGTTTAAAGTCATACCAAAGCGATGGGTTGTAGAACGTTCGTTTGCTTGGCTTGAGAAAAATAGACGTCTATGGAAAAACTGTGAGCGTC
TTTTAAATTCGAGCCTTCAATTTACCAATCTTGCTTTCATCGCTTTGTTGCTGAAAAGATTGTGAACAGGTTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 70 | 372 | + | No |
Description : First part of the transposase
ORF sequence :
MRKAYPSDISRKQFEHILPILESARKKTKPRTVDLYDVFCGLLYVLRTGCQWRQLPHDFPKWRTVHSYFQKWSELDEEGNSILDQALKKIGQKSAKEGQT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 534 bp | 177 aa | 332 | 865 | + | No |
Description : Second part of the transposase
ORF sequence :
KKLVKKARKKDKRKSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRG
EAFANSVKTLLGETVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
EAFANSVKTLLGETVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 796 bp | 264 aa | 70 | 865 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKAYPSDISRKQFEHILPILESARKKTKPRTVDLYDVFCGLLYVLRTGCQWRQLPHDFPKWRTVHSYFQKWSELDEEGNSILDQALKKLVKKARKKDKR
KSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRGEAFANSVKTLLGE
TVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
KSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRGEAFANSVKTLLGE
TVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
Blast result :
Comments
ISAba15 element was identified in the colistin-resistant South Korean clinical A. baumannii isolate B0707-070.
ISAba15 is 81% aa similar to ISNieu4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba15 is 81% aa similar to ISNieu4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References