ISEc36
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JN412066 | ND | Escherichia coli | Escherichia coli W635 |
DNA section
IS Length : 1321 bp
Ends
IR Length : 28/37
IRL : TGGCCCCATGAATCTCCAGACAGTTGGTATCACTTAAGTTAGTGATAGTC
IRR : TGGCCCCTATATTTCCATACACTTTTTATCACTTAACCCATTACTGGTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGACTGGAT | GTCTAATTCC | 0 |
DNA sequence
TGGCCCCATGAATCTCCAGACAGTTGGTATCACTTAAGTTAGTGATAGTCTTAATACTAGTTTTTAGACTATTCGTTGGAGTTCGGATGATTGATGTTTT
AGGTCCTGAGAAGGGCAGACGGCGCAGCGTTCAGGAAAAAATCGCCATTGTTCAGCAGAGTTTTGAACCCGGAATGACCGTGTCGCTGGTCGCCCGTCAG
CATGGTGTTGCTGCCAGTCAGCTGTTCCTGTGGCGTAAACAATATCAGGAAGGCAGCCTCACAGCCGTTGCCGCAGGAGAACAGGTTGTACCCGCGTCGG
AGCTGGCATCTGCGATGAAGCAAATTAAAGAGCTGCAGCGCCTGTTGGGCAAGAAAACCATGGAAAACGAGCTCCTAAAAGAAGCCGTTGAATATGGCCG
ACAAAAAAAGTGGATAGCGCACGTGCCCTTGTTGCCGGAGGATGGCGAATAAGCCTTGTCAGTCGTTGCCTCCGGGTCTCACGTGCGCAACTGCATGCCA
TGGCCCGTCGGTCGAAAGACTGGCAGGATCGTCGGTGCAAGCGCAAGCCTGATGATACTGACGCGCTGGCCCGTATCCATACCGTCATCGGCGATCTGCC
CACCTATGGTTATCGTCGGGTATGGGCACTGCTGCGCAGACAATCAGAAACTGACGACATGGCGGTGATCAATGCCAAACGCGTATACCGCATCATGCGT
CAGAATGCGCTGCTGCTTGAGCGTAAACCGGAAATACCGCTATCGAAGCGGGCGCATACAGGGAAAGTGGCCGTTGGAGAAAGTAACCAGCGGTGGTGCT
CTGACGGCTTCGAGTTCAGCTGTGATAACGGTGAAAAACTGCGGGTCACGTTCGCTCTGGACTGTTGCGATCGCGAGGCACTTCACTGGGCGGCCAGTAC
TGGTGGATATGACAGTGAAACCGTGCAGGACGTCATGCTGGGTGCCGTGGAGCGTCGCTTCGGTAACAGCCTGCCGACATCCCCCGTTGAGTGGCTGACA
GACAACGGTTCAGCCTACCGTTCTTATCAGACGCGTCAGTTCGCCAGAATGGTAGGACTGGAGCCTAAGCATACGGCGGTACGTAGCCCGGAAAGCAACG
GGATGGCAGAGAGCTTCGTGAAAACGATGAAGCGCGATTACATAAGTATCATGCCGAAACCCGACGGGTTAACAGCGGTAAAGAACCTCGCGGAGGCCTT
CGAACATTACAACGAATGGCATCCGCATAGTGCACTGGGGTATCGTTCGCCACGGGAATATCTGCGGCGACGAACCAGTAATGGGTTAAGTGATAAAAAG
TGTATGGAAATATAGGGGCCA
AGGTCCTGAGAAGGGCAGACGGCGCAGCGTTCAGGAAAAAATCGCCATTGTTCAGCAGAGTTTTGAACCCGGAATGACCGTGTCGCTGGTCGCCCGTCAG
CATGGTGTTGCTGCCAGTCAGCTGTTCCTGTGGCGTAAACAATATCAGGAAGGCAGCCTCACAGCCGTTGCCGCAGGAGAACAGGTTGTACCCGCGTCGG
AGCTGGCATCTGCGATGAAGCAAATTAAAGAGCTGCAGCGCCTGTTGGGCAAGAAAACCATGGAAAACGAGCTCCTAAAAGAAGCCGTTGAATATGGCCG
ACAAAAAAAGTGGATAGCGCACGTGCCCTTGTTGCCGGAGGATGGCGAATAAGCCTTGTCAGTCGTTGCCTCCGGGTCTCACGTGCGCAACTGCATGCCA
TGGCCCGTCGGTCGAAAGACTGGCAGGATCGTCGGTGCAAGCGCAAGCCTGATGATACTGACGCGCTGGCCCGTATCCATACCGTCATCGGCGATCTGCC
CACCTATGGTTATCGTCGGGTATGGGCACTGCTGCGCAGACAATCAGAAACTGACGACATGGCGGTGATCAATGCCAAACGCGTATACCGCATCATGCGT
CAGAATGCGCTGCTGCTTGAGCGTAAACCGGAAATACCGCTATCGAAGCGGGCGCATACAGGGAAAGTGGCCGTTGGAGAAAGTAACCAGCGGTGGTGCT
CTGACGGCTTCGAGTTCAGCTGTGATAACGGTGAAAAACTGCGGGTCACGTTCGCTCTGGACTGTTGCGATCGCGAGGCACTTCACTGGGCGGCCAGTAC
TGGTGGATATGACAGTGAAACCGTGCAGGACGTCATGCTGGGTGCCGTGGAGCGTCGCTTCGGTAACAGCCTGCCGACATCCCCCGTTGAGTGGCTGACA
GACAACGGTTCAGCCTACCGTTCTTATCAGACGCGTCAGTTCGCCAGAATGGTAGGACTGGAGCCTAAGCATACGGCGGTACGTAGCCCGGAAAGCAACG
GGATGGCAGAGAGCTTCGTGAAAACGATGAAGCGCGATTACATAAGTATCATGCCGAAACCCGACGGGTTAACAGCGGTAAAGAACCTCGCGGAGGCCTT
CGAACATTACAACGAATGGCATCCGCATAGTGCACTGGGGTATCGTTCGCCACGGGAATATCTGCGGCGACGAACCAGTAATGGGTTAAGTGATAAAAAG
TGTATGGAAATATAGGGGCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 411 bp | 136 aa | 42 | 452 | + | No |
Description : First part of the transposase
ORF sequence :
MIVLILVFRLFVGVRMIDVLGPEKGRRRSVQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELASAMKQIKELQR
LLGKKTMENELLKEAVEYGRQKKWIAHVPLLPEDGE
LLGKKTMENELLKEAVEYGRQKKWIAHVPLLPEDGE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 392 | 1315 | + | No |
Description : Second part of the transposase
ORF sequence :
IWPTKKVDSARALVAGGWRISLVSRCLRVSRAQLHAMARRSKDWQDRRCKRKPDDTDALARIHTVIGDLPTYGYRRVWALLRRQSETDDMAVINAKRVYR
IMRQNALLLERKPEIPLSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALHWAASTGGYDSETVQDVMLGAVERRFGNSLPTSPVE
WLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNGLS
DKKCMEI
IMRQNALLLERKPEIPLSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALHWAASTGGYDSETVQDVMLGAVERRFGNSLPTSPVE
WLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNGLS
DKKCMEI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1274 bp | 424 aa | 42 | 1315 | + | Yes |
Chemistry : DDE
ORF sequence :
MIVLILVFRLFVGVRMIDVLGPEKGRRRSVQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELASAMKQIKELQR
LLGKKTMENELLKEAVEYGRQKKVDSARALVAGGWRISLVSRCLRVSRAQLHAMARRSKDWQDRRCKRKPDDTDALARIHTVIGDLPTYGYRRVWALLRR
QSETDDMAVINAKRVYRIMRQNALLLERKPEIPLSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALHWAASTGGYDSETVQDVML
GAVERRFGNSLPTSPVEWLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALG
YRSPREYLRRRTSNGLSDKKCMEI
LLGKKTMENELLKEAVEYGRQKKVDSARALVAGGWRISLVSRCLRVSRAQLHAMARRSKDWQDRRCKRKPDDTDALARIHTVIGDLPTYGYRRVWALLRR
QSETDDMAVINAKRVYRIMRQNALLLERKPEIPLSKRAHTGKVAVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALHWAASTGGYDSETVQDVML
GAVERRFGNSLPTSPVEWLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGMAESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALG
YRSPREYLRRRTSNGLSDKKCMEI
Blast result :
Comments
ISEc36 is 91.2% (ORFA) and 98.9% (ORFB) aa similar to ISEc27. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References