ISAba14

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAba35
Accession numberTranspositionOriginHost
CP001921 ND Acinetobacter baumannii
Acinetobacter lwoffii
Acinetobacter baumannii MDR-ZJ06
Acinetobacter baumannii 1656-2
Acinetobacter baumannii strain A297 (RUH875)
DNA section
IS Length : 1282 bp

Ends


IR Length : 35/54

IRL : AGAGATGGATCCGTAAATTTGAACAACTCCTATAAGTGATATTCTGCTCC
IRR : AGAGGTGGTCCCACTTGTTTGAACAACTAAAAGCGTATTTATAAGTGATA

Insertion site


Left flankDirect repeatRight flankDR Length
TTATTACGAATTAATTAGAGCATA4
ACTCATGCCATAAAATTGTTTAAA4
GCACCAATCACACCACCTAAAGC3
CATTGCGGACTACCTAATTG0
TATGTTTAGACTCGCATTTG0

DNA sequence

AGAGATGGATCCGTAAATTTGAACAACTCCTATAAGTGATATTCTGCTCCTCAAATGATGTTATAAACATCAATATATGGAGTATTTTATGGCACGTAGA
CCAAGAAGAAATCATTCAAATGATTTTAAAGCTAAGGTAGCACTTGCTGCGATTAAAGCAGAAAAAACACTTGCTGAATTGAGTGCTGAGTTTGATGTTC
ATCAAAACCAAATTATTGACTGGAAAAATCAATTGATCTCAGCTTCCTCGCAAGCTTTCGATCAATCAAAAGCTCCAACAGAACCACCCATCGATCTAAA
AAAACTACATGCAAAAATCGGTGAGCAGGCATTAGAAATTGATTTTTTAGAAGGTGTGTTGAAGAAACTGGGCCGCTTCAACCACAAAAGTTAATCGACG
ACTCACTTCAGATTTCAGTATCTAAGCAAGCTAAGCTGCTGAAAGTCTCCCGTGGTTGTTATTACTATCGCCCAAAACCTGTGAGTGCATCAGATCTGAA
GCTGATGCGATGTATTGATGAATTACATATGCAATATCCTTTTGCAGGCAGTCGTATGATGCGTGATTTGTTGAATCGTCAAGGACATCATATAGGACGA
CGTCATACACGTACTTTAATGAAGAAAATGGGTATTCAGGCGTTATATTGCAAACCAAATTTAAGCCAGGCTAATCAAGCTCACCGTAAATATCCATATC
TGCTCAAAGGGTTGGCTATTCAGCGCAGTAATCAAGTGTGGTCTACGGATATAACGTATATCCCTATGGCAAAAGGCTTTGTTTATTTATGTGCTGTGAT
TGATTGGCATAGCCGCAAGGTACTTGCGCATAGGGTATCGATTAGTATGGAGGTGGATTTTTGTATTTCGGCTTTAAATGAAGCGATTGAAAAATATGGT
CGACCTGAAATATTTAATACAGACCAAGGCAGCCAGTTTACCAGTGATGCATTTATTGATGTATTGAAATCAAATGGCATTCAAATCAGTATGGATGGTA
AAGGTCGATGGGTAGATAATGTGATGGTTGAACGATTATGGCGGAGCGTTAAATATGAAGAGGTGTATCTCAAAGCTTATAGCAGTGTCACAGATGCGAA
AAAGCAATTAAGTGCATATTTTGAGTTTTATAATTTGAAACGACCTCATTCGAGTCTAGACAAAATGACACCAAATGAGTTTTACTATGATCAGCTACCC
CAACAAAACAAGGTGGCTTAACTAGAGCGGAATATCACTTATAAATACGCTTTTAGTTGTTCAAACAAGTGGGACCACCTCT
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
318 bp105 aa77394+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDWKNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIDFLEGVLKKLGR
FNHKS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
879 bp292 aa3431221+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

FFRRCVEETGPLQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGIQA
LYCKPNLSQANQAHRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFT
SDAFIDVLKSNGIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1145 bp381 aa771221+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDWKNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIDFFRRCVEETGP
LQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGIQALYCKPNLSQAN
QAHRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFTSDAFIDVLKSN
GIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA

 

Blast result :
Comments
ISAba14 is 75% aa similar to ISKpn11.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

The file was corrected by Sally Partridge indication the 24/04/2012.
References
1] Vaida Seputiene (2011) Direct submission.
2] Kim,J., Kim,S.-H., Kim,S.-M., Park,J.-Y., Lee,H.-W. and Shin,J.-H. (2010) Direct submission GenBank.