ISAba14
- Family IS3
- Group IS150
Isoform Synonym(s) ISAba35
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001921 | ND | Acinetobacter baumannii | Acinetobacter lwoffii Acinetobacter baumannii MDR-ZJ06 Acinetobacter baumannii 1656-2 Acinetobacter baumannii strain A297 (RUH875) |
DNA section
IS Length : 1282 bp
Ends
IR Length : 35/54
IRL : AGAGATGGATCCGTAAATTTGAACAACTCCTATAAGTGATATTCTGCTCC
IRR : AGAGGTGGTCCCACTTGTTTGAACAACTAAAAGCGTATTTATAAGTGATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTATTACGAA | TTAA | TTAGAGCATA | 4 |
| ACTCATGCCA | TAAA | ATTGTTTAAA | 4 |
| GCACCAATCA | CAC | CACCTAAAGC | 3 |
| CATTGCGGAC | TACCTAATTG | 0 | |
| TATGTTTAGA | CTCGCATTTG | 0 |
DNA sequence
AGAGATGGATCCGTAAATTTGAACAACTCCTATAAGTGATATTCTGCTCCTCAAATGATGTTATAAACATCAATATATGGAGTATTTTATGGCACGTAGA
CCAAGAAGAAATCATTCAAATGATTTTAAAGCTAAGGTAGCACTTGCTGCGATTAAAGCAGAAAAAACACTTGCTGAATTGAGTGCTGAGTTTGATGTTC
ATCAAAACCAAATTATTGACTGGAAAAATCAATTGATCTCAGCTTCCTCGCAAGCTTTCGATCAATCAAAAGCTCCAACAGAACCACCCATCGATCTAAA
AAAACTACATGCAAAAATCGGTGAGCAGGCATTAGAAATTGATTTTTTAGAAGGTGTGTTGAAGAAACTGGGCCGCTTCAACCACAAAAGTTAATCGACG
ACTCACTTCAGATTTCAGTATCTAAGCAAGCTAAGCTGCTGAAAGTCTCCCGTGGTTGTTATTACTATCGCCCAAAACCTGTGAGTGCATCAGATCTGAA
GCTGATGCGATGTATTGATGAATTACATATGCAATATCCTTTTGCAGGCAGTCGTATGATGCGTGATTTGTTGAATCGTCAAGGACATCATATAGGACGA
CGTCATACACGTACTTTAATGAAGAAAATGGGTATTCAGGCGTTATATTGCAAACCAAATTTAAGCCAGGCTAATCAAGCTCACCGTAAATATCCATATC
TGCTCAAAGGGTTGGCTATTCAGCGCAGTAATCAAGTGTGGTCTACGGATATAACGTATATCCCTATGGCAAAAGGCTTTGTTTATTTATGTGCTGTGAT
TGATTGGCATAGCCGCAAGGTACTTGCGCATAGGGTATCGATTAGTATGGAGGTGGATTTTTGTATTTCGGCTTTAAATGAAGCGATTGAAAAATATGGT
CGACCTGAAATATTTAATACAGACCAAGGCAGCCAGTTTACCAGTGATGCATTTATTGATGTATTGAAATCAAATGGCATTCAAATCAGTATGGATGGTA
AAGGTCGATGGGTAGATAATGTGATGGTTGAACGATTATGGCGGAGCGTTAAATATGAAGAGGTGTATCTCAAAGCTTATAGCAGTGTCACAGATGCGAA
AAAGCAATTAAGTGCATATTTTGAGTTTTATAATTTGAAACGACCTCATTCGAGTCTAGACAAAATGACACCAAATGAGTTTTACTATGATCAGCTACCC
CAACAAAACAAGGTGGCTTAACTAGAGCGGAATATCACTTATAAATACGCTTTTAGTTGTTCAAACAAGTGGGACCACCTCT
CCAAGAAGAAATCATTCAAATGATTTTAAAGCTAAGGTAGCACTTGCTGCGATTAAAGCAGAAAAAACACTTGCTGAATTGAGTGCTGAGTTTGATGTTC
ATCAAAACCAAATTATTGACTGGAAAAATCAATTGATCTCAGCTTCCTCGCAAGCTTTCGATCAATCAAAAGCTCCAACAGAACCACCCATCGATCTAAA
AAAACTACATGCAAAAATCGGTGAGCAGGCATTAGAAATTGATTTTTTAGAAGGTGTGTTGAAGAAACTGGGCCGCTTCAACCACAAAAGTTAATCGACG
ACTCACTTCAGATTTCAGTATCTAAGCAAGCTAAGCTGCTGAAAGTCTCCCGTGGTTGTTATTACTATCGCCCAAAACCTGTGAGTGCATCAGATCTGAA
GCTGATGCGATGTATTGATGAATTACATATGCAATATCCTTTTGCAGGCAGTCGTATGATGCGTGATTTGTTGAATCGTCAAGGACATCATATAGGACGA
CGTCATACACGTACTTTAATGAAGAAAATGGGTATTCAGGCGTTATATTGCAAACCAAATTTAAGCCAGGCTAATCAAGCTCACCGTAAATATCCATATC
TGCTCAAAGGGTTGGCTATTCAGCGCAGTAATCAAGTGTGGTCTACGGATATAACGTATATCCCTATGGCAAAAGGCTTTGTTTATTTATGTGCTGTGAT
TGATTGGCATAGCCGCAAGGTACTTGCGCATAGGGTATCGATTAGTATGGAGGTGGATTTTTGTATTTCGGCTTTAAATGAAGCGATTGAAAAATATGGT
CGACCTGAAATATTTAATACAGACCAAGGCAGCCAGTTTACCAGTGATGCATTTATTGATGTATTGAAATCAAATGGCATTCAAATCAGTATGGATGGTA
AAGGTCGATGGGTAGATAATGTGATGGTTGAACGATTATGGCGGAGCGTTAAATATGAAGAGGTGTATCTCAAAGCTTATAGCAGTGTCACAGATGCGAA
AAAGCAATTAAGTGCATATTTTGAGTTTTATAATTTGAAACGACCTCATTCGAGTCTAGACAAAATGACACCAAATGAGTTTTACTATGATCAGCTACCC
CAACAAAACAAGGTGGCTTAACTAGAGCGGAATATCACTTATAAATACGCTTTTAGTTGTTCAAACAAGTGGGACCACCTCT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 77 | 394 | + | No |
Description : First part of the transposase
ORF sequence :
MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDWKNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIDFLEGVLKKLGR
FNHKS
FNHKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 879 bp | 292 aa | 343 | 1221 | + | No |
Description : Second part of the transposase
ORF sequence :
FFRRCVEETGPLQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGIQA
LYCKPNLSQANQAHRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFT
SDAFIDVLKSNGIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA
LYCKPNLSQANQAHRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFT
SDAFIDVLKSNGIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1145 bp | 381 aa | 77 | 1221 | + | Yes |
Chemistry : DDE
ORF sequence :
MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDWKNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIDFFRRCVEETGP
LQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGIQALYCKPNLSQAN
QAHRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFTSDAFIDVLKSN
GIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA
LQPQKLIDDSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGIQALYCKPNLSQAN
QAHRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFTSDAFIDVLKSN
GIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA
Blast result :
Comments
ISAba14 is 75% aa similar to ISKpn11.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The file was corrected by Sally Partridge indication the 24/04/2012.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The file was corrected by Sally Partridge indication the 24/04/2012.
References
1] Vaida Seputiene (2011) Direct submission.
2] Kim,J., Kim,S.-H., Kim,S.-M., Park,J.-Y., Lee,H.-W. and Shin,J.-H. (2010) Direct submission GenBank.
2] Kim,J., Kim,S.-H., Kim,S.-M., Park,J.-Y., Lee,H.-W. and Shin,J.-H. (2010) Direct submission GenBank.