ISAba17
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter baumannii | Acinetobacter baumannii A91 |
DNA section
IS Length : 2594 bp
Ends
IR Length : 19/21
IRL : GTAAGCGTCCGCTGAACCCCACTTTTTCTAACTCATTAATACCGCGATAG
IRR : GTAAGCATCCGCTGAACGCCATCGCCAAGTTTTAACGGGGTTCAGGTTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAAATATCAT | TAATCATG | TATTGGTTTG | 8 |
DNA sequence
GTAAGCGTCCGCTGAACCCCACTTTTTCTAACTCATTAATACCGCGATAGTGTCCACTTAAATTTAAGTGGACACCTATTTATGGATTTAAAGACAGTTA
TAGACAGTGCACCAACGCTAAAAAAGCCCCGTCGAACCTTCACAGCTGAATTTAAACATCAACTTATTCAGCAATGCCAGCAGCCAGATACATCAGTGGC
CAAGGTAGCAATGCAACATCAGATCAATGCCAATCTGCTACATAAATGGATTCGTCAGTCCAGATCAATGCCCCCTGCATTAAAAACCCCATCCATTCCA
CAGACTGACTTTCTTCCGGTCATTCTTCACCCGACTCCAGTCAAACAAGAAGCACCTCTACCACCTGTGCCAGAGAAGAAAGCTGTCGCTCATATCAGGA
TTCCATTACATGAGGCACCAAGTTCCGCAAGGGATCAAATGATCGAGATCGACTGGCCGGTGGAGTCAGTAACTGAATTACTGTTGCTGATCCAGGGTTT
GACTCAATGATTCGTATTGATGAGATCTGGTTGTCTACCCAGCCGATGGACATGCGTGCAGGGATGGATACAGCCATGGCTCAGGTGCTGAGAGCCTTTG
GCTACATCAAACCGCATTGCGCTTACCTGTTCTGTAATAAGCGTGGCCATCGTATGAAAGTGCTGGTGCATGATGGACTGGGTATCTGGCTGTGTGCCCG
GCGGCTGGAACAGGGGAAATTCCACTGGGCTAAAGTTCACCAAGGTGAAAGTATTGCGATCAGCCCGGAGCAGTTACAGGCACTGATCCAGGGTTTGCCC
TGGCAAAGAATCGGACTGCAGCAAGTGGTAACAATGCTCTAAACCAGGCTCATCCATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCATCATGACCT
CAGGCATACTGCGGTCATGAATACGCTGCCTGATTTAAGCCAACTGACCCATGAACAACTGCTGGAATTTACCAGACAGTTGGCGATGCAGCATCAGTCT
CTGGCACAATCTAAACAACAACTGGAACAATCAAATCAGCAATTAGATGCCAGAGTTCAACATCTTGAAGTCACCAATCAGCAATTAGATTCTAAAGTTC
AACATCTTTCTATTCTCAATCAAAAATACGAGCATGAGCTTGCACTATTTAAACAGCATAAATTCGGCAGTAAAAATGAACATCTAACCGCAAAACAAAT
CCATCTCTGGGATGAAGCGGTTGAAGAAGATATTGCAGCGGTTGATCTGGAACTGGAACGACTGAATGCAGATAAAACCAATGCAGCTGCACAGAAAGCC
CCAGTCAACAAACCTAAACGTCGGCTGTTGCCAGATCATCTACACACCCTCCGTATTGAGCATGAACCTGAATCAACACAATGCAGTTGTGGCTGTACCT
TGCGTCGTATCGGTGAAGATGTCAGTGAAAAACTGAATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAAATGGGTCTGTGATCAGTG
TGACACTCTGACTCAGCAAGCGATGCCAGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAATTGCTTAGCCATGTGCTGGTGTCAAAGTATGCCGAT
CATTTGCCTTTGTACCGTCAACGTCAAATCTTTCTACGCGCAGGTGTTGATCTGTCTAGATCAACGTTATCTGACTGGATTGGCCGCTGTGGGGTGGAAC
TGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTACTGCAACAGCAGGTGATCCATGCAGATGAAACACCGGTGACGGTCATGCAGATGGGTGAAAATGA
GAAAAAGCCCAAAAAAGGTTATGTCTGGGCCTATGCCAGCACACAGTACAATCCAGTTCAGGCAGTGATCTATGACTTTCAAGATAGCCGTTCTGGCCAG
CATGCTGAAGACTTCCTGAAAGGCTGGCAGGGTCATTTGGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAGATCAGGTCAGGTCATTGAGGTGG
GCTGCATGGCACATGCACGTCGTAAATTCCATGAACTACATGTGACCAAGAAAAGTCAGGTCGCTGAACAGGCATTAGTGCTGATTCAGAAATTATATGC
GATAGAAGCAGAATTAAGAAAAAAGACGGATGGTACAGCGGAAGACCGCTGCGAATACCGACAACAGCATAGTCAACCGGTCATGCAACAACTATATGAA
TGGCTCAACCAACATCAACTGACGGTGCCATCGAGTTCTCCAACCGCCCGGGCGATCAATTACAGCCTAAAACGTTGGACTGCTTTAAGCCGCTATCTGG
ATGATGGCAATCTACCCATTGACAATAATTGGATAGAGAACCAAATGCGTCCCTGGGCCTTGGGGCGTAAGAACTGGCTATTTGCAGGTTCGCTGCGCAG
TGGTCAACGAGCTGCCAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGATGTGCTGAAAAGGTTGCCA
ACACATAAAGTGCCTCAAATAGAAGAATTACTACCTCACCGCTGGAAACCTGAACCCCGTTAAAACTTGGCGATGGCGTTCAGCGGATGCTTAC
TAGACAGTGCACCAACGCTAAAAAAGCCCCGTCGAACCTTCACAGCTGAATTTAAACATCAACTTATTCAGCAATGCCAGCAGCCAGATACATCAGTGGC
CAAGGTAGCAATGCAACATCAGATCAATGCCAATCTGCTACATAAATGGATTCGTCAGTCCAGATCAATGCCCCCTGCATTAAAAACCCCATCCATTCCA
CAGACTGACTTTCTTCCGGTCATTCTTCACCCGACTCCAGTCAAACAAGAAGCACCTCTACCACCTGTGCCAGAGAAGAAAGCTGTCGCTCATATCAGGA
TTCCATTACATGAGGCACCAAGTTCCGCAAGGGATCAAATGATCGAGATCGACTGGCCGGTGGAGTCAGTAACTGAATTACTGTTGCTGATCCAGGGTTT
GACTCAATGATTCGTATTGATGAGATCTGGTTGTCTACCCAGCCGATGGACATGCGTGCAGGGATGGATACAGCCATGGCTCAGGTGCTGAGAGCCTTTG
GCTACATCAAACCGCATTGCGCTTACCTGTTCTGTAATAAGCGTGGCCATCGTATGAAAGTGCTGGTGCATGATGGACTGGGTATCTGGCTGTGTGCCCG
GCGGCTGGAACAGGGGAAATTCCACTGGGCTAAAGTTCACCAAGGTGAAAGTATTGCGATCAGCCCGGAGCAGTTACAGGCACTGATCCAGGGTTTGCCC
TGGCAAAGAATCGGACTGCAGCAAGTGGTAACAATGCTCTAAACCAGGCTCATCCATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCATCATGACCT
CAGGCATACTGCGGTCATGAATACGCTGCCTGATTTAAGCCAACTGACCCATGAACAACTGCTGGAATTTACCAGACAGTTGGCGATGCAGCATCAGTCT
CTGGCACAATCTAAACAACAACTGGAACAATCAAATCAGCAATTAGATGCCAGAGTTCAACATCTTGAAGTCACCAATCAGCAATTAGATTCTAAAGTTC
AACATCTTTCTATTCTCAATCAAAAATACGAGCATGAGCTTGCACTATTTAAACAGCATAAATTCGGCAGTAAAAATGAACATCTAACCGCAAAACAAAT
CCATCTCTGGGATGAAGCGGTTGAAGAAGATATTGCAGCGGTTGATCTGGAACTGGAACGACTGAATGCAGATAAAACCAATGCAGCTGCACAGAAAGCC
CCAGTCAACAAACCTAAACGTCGGCTGTTGCCAGATCATCTACACACCCTCCGTATTGAGCATGAACCTGAATCAACACAATGCAGTTGTGGCTGTACCT
TGCGTCGTATCGGTGAAGATGTCAGTGAAAAACTGAATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAAATGGGTCTGTGATCAGTG
TGACACTCTGACTCAGCAAGCGATGCCAGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAATTGCTTAGCCATGTGCTGGTGTCAAAGTATGCCGAT
CATTTGCCTTTGTACCGTCAACGTCAAATCTTTCTACGCGCAGGTGTTGATCTGTCTAGATCAACGTTATCTGACTGGATTGGCCGCTGTGGGGTGGAAC
TGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTACTGCAACAGCAGGTGATCCATGCAGATGAAACACCGGTGACGGTCATGCAGATGGGTGAAAATGA
GAAAAAGCCCAAAAAAGGTTATGTCTGGGCCTATGCCAGCACACAGTACAATCCAGTTCAGGCAGTGATCTATGACTTTCAAGATAGCCGTTCTGGCCAG
CATGCTGAAGACTTCCTGAAAGGCTGGCAGGGTCATTTGGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAGATCAGGTCAGGTCATTGAGGTGG
GCTGCATGGCACATGCACGTCGTAAATTCCATGAACTACATGTGACCAAGAAAAGTCAGGTCGCTGAACAGGCATTAGTGCTGATTCAGAAATTATATGC
GATAGAAGCAGAATTAAGAAAAAAGACGGATGGTACAGCGGAAGACCGCTGCGAATACCGACAACAGCATAGTCAACCGGTCATGCAACAACTATATGAA
TGGCTCAACCAACATCAACTGACGGTGCCATCGAGTTCTCCAACCGCCCGGGCGATCAATTACAGCCTAAAACGTTGGACTGCTTTAAGCCGCTATCTGG
ATGATGGCAATCTACCCATTGACAATAATTGGATAGAGAACCAAATGCGTCCCTGGGCCTTGGGGCGTAAGAACTGGCTATTTGCAGGTTCGCTGCGCAG
TGGTCAACGAGCTGCCAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGATGTGCTGAAAAGGTTGCCA
ACACATAAAGTGCCTCAAATAGAAGAATTACTACCTCACCGCTGGAAACCTGAACCCCGTTAAAACTTGGCGATGGCGTTCAGCGGATGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 429 bp | 142 aa | 82 | 510 | + | No |
AG : IS66 TnpA
ORF sequence :
MDLKTVIDSAPTLKKPRRTFTAEFKHQLIQQCQQPDTSVAKVAMQHQINANLLHKWIRQSRSMPPALKTPSIPQTDFLPVILHPTPVKQEAPLPPVPEKK
AVAHIRIPLHEAPSSARDQMIEIDWPVESVTELLLLIQGLTQ
AVAHIRIPLHEAPSSARDQMIEIDWPVESVTELLLLIQGLTQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 336 bp | 111 aa | 507 | 842 | + | No |
AG : IS66 TnpB
ORF sequence :
MIRIDEIWLSTQPMDMRAGMDTAMAQVLRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAKVHQGESIAISPEQLQALIQGLPWQ
RIGLQQVVTML
RIGLQQVVTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1647 bp | 548 aa | 917 | 2563 | + | No |
Chemistry : DDE
ORF sequence :
MNTLPDLSQLTHEQLLEFTRQLAMQHQSLAQSKQQLEQSNQQLDARVQHLEVTNQQLDSKVQHLSILNQKYEHELALFKQHKFGSKNEHLTAKQIHLWDE
AVEEDIAAVDLELERLNADKTNAAAQKAPVNKPKRRLLPDHLHTLRIEHEPESTQCSCGCTLRRIGEDVSEKLNFRPAQFYKEQHVRGKWVCDQCDTLTQ
QAMPAYVIDKGIASPELLSHVLVSKYADHLPLYRQRQIFLRAGVDLSRSTLSDWIGRCGVELEPLANALKQVVLQQQVIHADETPVTVMQMGENEKKPKK
GYVWAYASTQYNPVQAVIYDFQDSRSGQHAEDFLKGWQGHLVCDDYSGYKARFRSGQVIEVGCMAHARRKFHELHVTKKSQVAEQALVLIQKLYAIEAEL
RKKTDGTAEDRCEYRQQHSQPVMQQLYEWLNQHQLTVPSSSPTARAINYSLKRWTALSRYLDDGNLPIDNNWIENQMRPWALGRKNWLFAGSLRSGQRAA
NIMTLIQSAKLNGLDPYAYLSDVLKRLPTHKVPQIEELLPHRWKPEPR
AVEEDIAAVDLELERLNADKTNAAAQKAPVNKPKRRLLPDHLHTLRIEHEPESTQCSCGCTLRRIGEDVSEKLNFRPAQFYKEQHVRGKWVCDQCDTLTQ
QAMPAYVIDKGIASPELLSHVLVSKYADHLPLYRQRQIFLRAGVDLSRSTLSDWIGRCGVELEPLANALKQVVLQQQVIHADETPVTVMQMGENEKKPKK
GYVWAYASTQYNPVQAVIYDFQDSRSGQHAEDFLKGWQGHLVCDDYSGYKARFRSGQVIEVGCMAHARRKFHELHVTKKSQVAEQALVLIQKLYAIEAEL
RKKTDGTAEDRCEYRQQHSQPVMQQLYEWLNQHQLTVPSSSPTARAINYSLKRWTALSRYLDDGNLPIDNNWIENQMRPWALGRKNWLFAGSLRSGQRAA
NIMTLIQSAKLNGLDPYAYLSDVLKRLPTHKVPQIEELLPHRWKPEPR
Blast result :
Comments
ISAba17 is 61% (ORFA), 78% (ORFB) and 67% (ORFC : the transposase) aa similar to ISPpu19.
References
1] Nigro, S.J. and Hall, R.M.(2011) Direct submission